Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea
Background: Minor saponins or human intestinal bacterial metabolites, such as ginsenosides Rg3, F2, Rh2, and compound K, are more pharmacologically active than major saponins, such as ginsenosides Rb1, Rb2, and Rc. In this work, enzymatic hydrolysis of ginsenoside Rb1 was studied using enzyme prepar...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Journal of Ginseng Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315000500 |
| _version_ | 1811313427628425216 |
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| author | Jitendra Upadhyaya Min-Ji Kim Young-Hoi Kim Sung-Ryong Ko Hee-Won Park Myung-Kon Kim |
| author_facet | Jitendra Upadhyaya Min-Ji Kim Young-Hoi Kim Sung-Ryong Ko Hee-Won Park Myung-Kon Kim |
| author_sort | Jitendra Upadhyaya |
| collection | DOAJ |
| description | Background: Minor saponins or human intestinal bacterial metabolites, such as ginsenosides Rg3, F2, Rh2, and compound K, are more pharmacologically active than major saponins, such as ginsenosides Rb1, Rb2, and Rc. In this work, enzymatic hydrolysis of ginsenoside Rb1 was studied using enzyme preparations from cultured mycelia of mushrooms.
Methods: Mycelia of Armillaria mellea, Ganoderma lucidum, Phellinus linteus, Elfvingia applanata, and Pleurotus ostreatus were cultivated in liquid media at 25°C for 2 wk. Enzyme preparations from cultured mycelia of five mushrooms were obtained by mycelia separation from cultured broth, enzyme extraction, ammonium sulfate (30–80%) precipitation, dialysis, and freeze drying, respectively. The enzyme preparations were used for enzymatic hydrolysis of ginsenoside Rb1.
Results: Among the mushrooms used in this study, the enzyme preparation from cultured mycelia of A. mellea (AMMEP) was found to convert ginsenoside Rb1 into compound K with a high yield, while those from G. lucidum, P. linteus, E. applanata, and P. ostreatus produced remarkable amounts of ginsenoside Rd from ginsenoside Rb1. The enzymatic hydrolysis pathway of ginsenoside Rb1 by AMMEP was Rb1 → Rd → F2 → compound K. The optimum reaction conditions for compound K formation from ginsenoside Rb1 were as follows: reaction time 72–96 h, pH 4.0–4.5, and temperature 45–55°C.
Conclusion: AMMEP can be used to produce the human intestinal bacterial metabolite, compound K, from ginsenoside Rb1 with a high yield and without food safety issues. |
| first_indexed | 2024-04-13T10:54:49Z |
| format | Article |
| id | doaj.art-9eb4c017482844c88d71f90d33bbf2d5 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-13T10:54:49Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-9eb4c017482844c88d71f90d33bbf2d52022-12-22T02:49:34ZengElsevierJournal of Ginseng Research1226-84532016-04-0140210511210.1016/j.jgr.2015.05.007Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria melleaJitendra Upadhyaya0Min-Ji Kim1Young-Hoi Kim2Sung-Ryong Ko3Hee-Won Park4Myung-Kon Kim5Department of Food Science and Technology, Chonbuk National University, Iksan, KoreaDepartment of Food Science and Technology, Chonbuk National University, Iksan, KoreaDepartment of Food Science and Technology, Chonbuk National University, Iksan, KoreaBureau of General Affairs, The Korean Society of Ginseng, Seoul, KoreaKorea Ginseng Corporation Research Institute, Korea Ginseng Corporation, Daejeon, KoreaDepartment of Food Science and Technology, Chonbuk National University, Iksan, KoreaBackground: Minor saponins or human intestinal bacterial metabolites, such as ginsenosides Rg3, F2, Rh2, and compound K, are more pharmacologically active than major saponins, such as ginsenosides Rb1, Rb2, and Rc. In this work, enzymatic hydrolysis of ginsenoside Rb1 was studied using enzyme preparations from cultured mycelia of mushrooms. Methods: Mycelia of Armillaria mellea, Ganoderma lucidum, Phellinus linteus, Elfvingia applanata, and Pleurotus ostreatus were cultivated in liquid media at 25°C for 2 wk. Enzyme preparations from cultured mycelia of five mushrooms were obtained by mycelia separation from cultured broth, enzyme extraction, ammonium sulfate (30–80%) precipitation, dialysis, and freeze drying, respectively. The enzyme preparations were used for enzymatic hydrolysis of ginsenoside Rb1. Results: Among the mushrooms used in this study, the enzyme preparation from cultured mycelia of A. mellea (AMMEP) was found to convert ginsenoside Rb1 into compound K with a high yield, while those from G. lucidum, P. linteus, E. applanata, and P. ostreatus produced remarkable amounts of ginsenoside Rd from ginsenoside Rb1. The enzymatic hydrolysis pathway of ginsenoside Rb1 by AMMEP was Rb1 → Rd → F2 → compound K. The optimum reaction conditions for compound K formation from ginsenoside Rb1 were as follows: reaction time 72–96 h, pH 4.0–4.5, and temperature 45–55°C. Conclusion: AMMEP can be used to produce the human intestinal bacterial metabolite, compound K, from ginsenoside Rb1 with a high yield and without food safety issues.http://www.sciencedirect.com/science/article/pii/S1226845315000500Armillaria melleacompound Kenzymatic hydrolysisginsenoside Rb1 |
| spellingShingle | Jitendra Upadhyaya Min-Ji Kim Young-Hoi Kim Sung-Ryong Ko Hee-Won Park Myung-Kon Kim Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea Journal of Ginseng Research Armillaria mellea compound K enzymatic hydrolysis ginsenoside Rb1 |
| title | Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea |
| title_full | Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea |
| title_fullStr | Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea |
| title_full_unstemmed | Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea |
| title_short | Enzymatic formation of compound-K from ginsenoside Rb1 by enzyme preparation from cultured mycelia of Armillaria mellea |
| title_sort | enzymatic formation of compound k from ginsenoside rb1 by enzyme preparation from cultured mycelia of armillaria mellea |
| topic | Armillaria mellea compound K enzymatic hydrolysis ginsenoside Rb1 |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315000500 |
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