Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury
Exposure to noise or ototoxic agents can result in degeneration of cells in the sensory epithelium and auditory nerve, as well as non-sensory cells of the cochlear lateral wall. However, the molecular mechanisms underlying this pathology remain unclear. The purpose of this study was to localize and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnmol.2018.00243/full |
| _version_ | 1819020403611271168 |
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| author | Kenyaria V. Noble Michelle L. Reyzer Jeremy L. Barth Hayes McDonald Michael Tuck Kevin L. Schey Edward L. Krug Hainan Lang |
| author_facet | Kenyaria V. Noble Michelle L. Reyzer Jeremy L. Barth Hayes McDonald Michael Tuck Kevin L. Schey Edward L. Krug Hainan Lang |
| author_sort | Kenyaria V. Noble |
| collection | DOAJ |
| description | Exposure to noise or ototoxic agents can result in degeneration of cells in the sensory epithelium and auditory nerve, as well as non-sensory cells of the cochlear lateral wall. However, the molecular mechanisms underlying this pathology remain unclear. The purpose of this study was to localize and identify proteins in the cochlea that are responsive to noise or ototoxic exposure using a complementary proteo-transcriptomic approach. MALDI imaging of cochlear sections revealed numerous protein signals with distinct cochlear localization patterns in both cochlear injury models, of which six were chosen for further investigation. A query of proteomic databases identified 709 candidates corresponding to m/z values for the six proteins. An evaluation of mRNA expression data from our previous studies of these injured models indicated that 208 of the candidates were affected in both injury models. Downstream validation analyses yielded proteins with confirmatory distributions and responses to injury. The combined analysis of MALDI imaging with gene expression data provides a new strategy to identify molecular regulators responsive to cochlear injury. This study demonstrates the applicability of MALDI imaging for investigating protein localization and abundance in frozen sections from animals modeling cochlear pathology. |
| first_indexed | 2024-12-21T03:50:39Z |
| format | Article |
| id | doaj.art-9eb865eabfad4e778317c0d8e780712c |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-21T03:50:39Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-9eb865eabfad4e778317c0d8e780712c2022-12-21T19:16:59ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992018-07-011110.3389/fnmol.2018.00243375462Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear InjuryKenyaria V. Noble0Michelle L. Reyzer1Jeremy L. Barth2Hayes McDonald3Michael Tuck4Kevin L. Schey5Edward L. Krug6Hainan Lang7Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, United StatesMass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United StatesDepartment of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, United StatesMass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United StatesMass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United StatesMass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United StatesDepartment of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, United StatesDepartment of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, United StatesExposure to noise or ototoxic agents can result in degeneration of cells in the sensory epithelium and auditory nerve, as well as non-sensory cells of the cochlear lateral wall. However, the molecular mechanisms underlying this pathology remain unclear. The purpose of this study was to localize and identify proteins in the cochlea that are responsive to noise or ototoxic exposure using a complementary proteo-transcriptomic approach. MALDI imaging of cochlear sections revealed numerous protein signals with distinct cochlear localization patterns in both cochlear injury models, of which six were chosen for further investigation. A query of proteomic databases identified 709 candidates corresponding to m/z values for the six proteins. An evaluation of mRNA expression data from our previous studies of these injured models indicated that 208 of the candidates were affected in both injury models. Downstream validation analyses yielded proteins with confirmatory distributions and responses to injury. The combined analysis of MALDI imaging with gene expression data provides a new strategy to identify molecular regulators responsive to cochlear injury. This study demonstrates the applicability of MALDI imaging for investigating protein localization and abundance in frozen sections from animals modeling cochlear pathology.https://www.frontiersin.org/article/10.3389/fnmol.2018.00243/fullcochleahearing lossMALDI-TOF IMSmicroarraynoiseouabain |
| spellingShingle | Kenyaria V. Noble Michelle L. Reyzer Jeremy L. Barth Hayes McDonald Michael Tuck Kevin L. Schey Edward L. Krug Hainan Lang Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury Frontiers in Molecular Neuroscience cochlea hearing loss MALDI-TOF IMS microarray noise ouabain |
| title | Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury |
| title_full | Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury |
| title_fullStr | Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury |
| title_full_unstemmed | Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury |
| title_short | Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury |
| title_sort | use of proteomic imaging coupled with transcriptomic analysis to identify biomolecules responsive to cochlear injury |
| topic | cochlea hearing loss MALDI-TOF IMS microarray noise ouabain |
| url | https://www.frontiersin.org/article/10.3389/fnmol.2018.00243/full |
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