THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION
Palmvitee may act as a beneficial supplement to cartilage tissue engineering which is currently still has certain limitation on chondrocyte expansion and dedifferentiation. Our objectives in thisstudy were to evaluate the effects of Palmvitee on the growth kinetic and phenotype gene expression of hu...
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| Format: | Article |
| Language: | English |
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Universiti Brunei Darussalam
2017-11-01
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| Series: | ASEAN Journal on Science and Technology for Development |
| Online Access: | http://www.ajstd.org/index.php/ajstd/article/view/159 |
| _version_ | 1827362004581482496 |
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| author | B.H.I. Ruszymah B.S. Aminuddin A. Gapor H. Fuzina |
| author_facet | B.H.I. Ruszymah B.S. Aminuddin A. Gapor H. Fuzina |
| author_sort | B.H.I. Ruszymah |
| collection | DOAJ |
| description | Palmvitee may act as a beneficial supplement to cartilage tissue engineering which is currently still has certain limitation on chondrocyte expansion and dedifferentiation. Our objectives in thisstudy were to evaluate the effects of Palmvitee on the growth kinetic and phenotype gene expression of human nasal septal chondrocytesin monolayer expansion as well as cartilage reconstruction via tissue engineering technology. Human chondrocytes were cultured inmediums containing various Palmvitee concentrations. Among the different test groups, the medium containing Palmvitee at 3 μg/ml supported the highest chondrocyte growth rate. The gene expression in monolayer chondrocytes culture supplemented with 3 μg/ml Palmviteedemonstrated similar results as in control. Cultured chondrocytes from the medium added with 3 μg/ml Palmvitee were then mixed with Pluronic F127 for cartilage reconstruction in nude mice model. Palmvitee supplementation supported the engineered cartilage development as shown in the histological and gene expression analysis on engineered tissue after 8 weeks of implantation. Therefore, Palmvitee at 3 μg/ml is beneficial for the human nasal septal chondrocytes monolayer expansion and cartilage engineering. |
| first_indexed | 2024-03-08T07:17:40Z |
| format | Article |
| id | doaj.art-9ebbd9b3c1e342cfa735b8637be1ce2d |
| institution | Directory Open Access Journal |
| issn | 0217-5460 2224-9028 |
| language | English |
| last_indexed | 2024-03-08T07:17:40Z |
| publishDate | 2017-11-01 |
| publisher | Universiti Brunei Darussalam |
| record_format | Article |
| series | ASEAN Journal on Science and Technology for Development |
| spelling | doaj.art-9ebbd9b3c1e342cfa735b8637be1ce2d2024-02-03T00:28:10ZengUniversiti Brunei DarussalamASEAN Journal on Science and Technology for Development0217-54602224-90282017-11-0122321122210.29037/ajstd.159154THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTIONB.H.I. Ruszymah0B.S. Aminuddin1A. Gapor2H. Fuzina3Department of Physiology, Medical Faculty of Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Az iz, 50300 Kuala LumpurTissue Engineering Laboratory, Hospital Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras, Kuala LumpurMalaysia Palm Oil Board, No 6, Persiaran Institusi, Bandar Baru Bangi 43000 Kajang SelangorAnimal Unit, Institute for Medical Re search, Jalan Pahang, 50588 Kuala LumpurPalmvitee may act as a beneficial supplement to cartilage tissue engineering which is currently still has certain limitation on chondrocyte expansion and dedifferentiation. Our objectives in thisstudy were to evaluate the effects of Palmvitee on the growth kinetic and phenotype gene expression of human nasal septal chondrocytesin monolayer expansion as well as cartilage reconstruction via tissue engineering technology. Human chondrocytes were cultured inmediums containing various Palmvitee concentrations. Among the different test groups, the medium containing Palmvitee at 3 μg/ml supported the highest chondrocyte growth rate. The gene expression in monolayer chondrocytes culture supplemented with 3 μg/ml Palmviteedemonstrated similar results as in control. Cultured chondrocytes from the medium added with 3 μg/ml Palmvitee were then mixed with Pluronic F127 for cartilage reconstruction in nude mice model. Palmvitee supplementation supported the engineered cartilage development as shown in the histological and gene expression analysis on engineered tissue after 8 weeks of implantation. Therefore, Palmvitee at 3 μg/ml is beneficial for the human nasal septal chondrocytes monolayer expansion and cartilage engineering.http://www.ajstd.org/index.php/ajstd/article/view/159 |
| spellingShingle | B.H.I. Ruszymah B.S. Aminuddin A. Gapor H. Fuzina THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION ASEAN Journal on Science and Technology for Development |
| title | THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION |
| title_full | THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION |
| title_fullStr | THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION |
| title_full_unstemmed | THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION |
| title_short | THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION |
| title_sort | effects of palmvitee on human nasal septal chondrocytes culture expansion and cartilage reconstruction |
| url | http://www.ajstd.org/index.php/ajstd/article/view/159 |
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