| Summary: | Pulpitis refers to the inflammation of dental pulp tissues caused by infection with
dental caries. We aimed to evaluate the effects of micro ribonucleic acid (miR)-155
on inflammatory response and autophagy upon pulpitis via the nucleotide-binding
oligomerization domain-like receptor protein 3 (NLRP3) signal. Forty rats were
randomly assigned to negative control (NC), pulpitis model (PM), anti-miR-155, and
anti-miR-155+diethyldithiocarbamate (DDC) groups (n=10). Primary human dental
pulp cells were divided into NC, lipopolysaccharide (LPS), anti-miR-155, and DDC
groups. Compared with the PM group, the IL-1β, TNF-α, and MDA levels and pulp
necrosis rate decreased, while the SOD activity was enhanced in the anti-miR-155 group
(P<0.05). Compared to the NC group, the positive expressions of LC3B and Beclin1
and the protein expressions of NLRP3 and Caspase-1 significantly rose in the PM
group (P<0.05). Compared with the PM group, the protein expressions of NLRP3 and
Caspase-1 significantly decreased, and the positive expressions of LC3B and Beclin1
increased in the anti-miR-155 group (P<0.05). Compared with the anti-miR-155 group,
the DDC group had significantly enhanced activity of dental pulp cells, up-regulated
mRNA levels of IL-1β, TNF-α, NLRP3, and Caspase-1, and decreased mRNA levels of
LC3B and Beclin1 (P<0.05). Suppressing miR-155 expression can relieve inflammatory
response and promote autophagy.
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