Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal
Pulpitis refers to the inflammation of dental pulp tissues caused by infection with dental caries. We aimed to evaluate the effects of micro ribonucleic acid (miR)-155 on inflammatory response and autophagy upon pulpitis via the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2023-09-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3021 |
| _version_ | 1797403854754545664 |
|---|---|
| author | Xin ZHANG Yidi JIANG Shiliang GUO |
| author_facet | Xin ZHANG Yidi JIANG Shiliang GUO |
| author_sort | Xin ZHANG |
| collection | DOAJ |
| description | Pulpitis refers to the inflammation of dental pulp tissues caused by infection with
dental caries. We aimed to evaluate the effects of micro ribonucleic acid (miR)-155
on inflammatory response and autophagy upon pulpitis via the nucleotide-binding
oligomerization domain-like receptor protein 3 (NLRP3) signal. Forty rats were
randomly assigned to negative control (NC), pulpitis model (PM), anti-miR-155, and
anti-miR-155+diethyldithiocarbamate (DDC) groups (n=10). Primary human dental
pulp cells were divided into NC, lipopolysaccharide (LPS), anti-miR-155, and DDC
groups. Compared with the PM group, the IL-1β, TNF-α, and MDA levels and pulp
necrosis rate decreased, while the SOD activity was enhanced in the anti-miR-155 group
(P<0.05). Compared to the NC group, the positive expressions of LC3B and Beclin1
and the protein expressions of NLRP3 and Caspase-1 significantly rose in the PM
group (P<0.05). Compared with the PM group, the protein expressions of NLRP3 and
Caspase-1 significantly decreased, and the positive expressions of LC3B and Beclin1
increased in the anti-miR-155 group (P<0.05). Compared with the anti-miR-155 group,
the DDC group had significantly enhanced activity of dental pulp cells, up-regulated
mRNA levels of IL-1β, TNF-α, NLRP3, and Caspase-1, and decreased mRNA levels of
LC3B and Beclin1 (P<0.05). Suppressing miR-155 expression can relieve inflammatory
response and promote autophagy. |
| first_indexed | 2024-03-09T02:44:30Z |
| format | Article |
| id | doaj.art-9ebef722bd2e472b8c8e3350636b9ca4 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-09T02:44:30Z |
| publishDate | 2023-09-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-9ebef722bd2e472b8c8e3350636b9ca42023-12-05T21:27:25ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-09-0129545546110.9775/kvfd.2023.293973021Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signalXin ZHANG0Yidi JIANG1Shiliang GUO2Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210018, Jiangsu Province, ChinaGuiyang Stomatological Hospital, Guiyang 550000, Guizhou Province, CHINANanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210018, Jiangsu Province, ChinaPulpitis refers to the inflammation of dental pulp tissues caused by infection with dental caries. We aimed to evaluate the effects of micro ribonucleic acid (miR)-155 on inflammatory response and autophagy upon pulpitis via the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) signal. Forty rats were randomly assigned to negative control (NC), pulpitis model (PM), anti-miR-155, and anti-miR-155+diethyldithiocarbamate (DDC) groups (n=10). Primary human dental pulp cells were divided into NC, lipopolysaccharide (LPS), anti-miR-155, and DDC groups. Compared with the PM group, the IL-1β, TNF-α, and MDA levels and pulp necrosis rate decreased, while the SOD activity was enhanced in the anti-miR-155 group (P<0.05). Compared to the NC group, the positive expressions of LC3B and Beclin1 and the protein expressions of NLRP3 and Caspase-1 significantly rose in the PM group (P<0.05). Compared with the PM group, the protein expressions of NLRP3 and Caspase-1 significantly decreased, and the positive expressions of LC3B and Beclin1 increased in the anti-miR-155 group (P<0.05). Compared with the anti-miR-155 group, the DDC group had significantly enhanced activity of dental pulp cells, up-regulated mRNA levels of IL-1β, TNF-α, NLRP3, and Caspase-1, and decreased mRNA levels of LC3B and Beclin1 (P<0.05). Suppressing miR-155 expression can relieve inflammatory response and promote autophagy.https://vetdergikafkas.org/pdf.php?id=3021autophagyinflammatory responsemicrornaspulpitis |
| spellingShingle | Xin ZHANG Yidi JIANG Shiliang GUO Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal Kafkas Universitesi Veteriner Fakültesi Dergisi autophagy inflammatory response micrornas pulpitis |
| title | Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal |
| title_full | Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal |
| title_fullStr | Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal |
| title_full_unstemmed | Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal |
| title_short | Effects of mirna-155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal |
| title_sort | effects of mirna 155 on inflammatory response and autophagy upon pulpitis through the nlrp3 signal |
| topic | autophagy inflammatory response micrornas pulpitis |
| url | https://vetdergikafkas.org/pdf.php?id=3021 |
| work_keys_str_mv | AT xinzhang effectsofmirna155oninflammatoryresponseandautophagyuponpulpitisthroughthenlrp3signal AT yidijiang effectsofmirna155oninflammatoryresponseandautophagyuponpulpitisthroughthenlrp3signal AT shiliangguo effectsofmirna155oninflammatoryresponseandautophagyuponpulpitisthroughthenlrp3signal |