Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae)
The Neotropical genus Harttia comprises species with extensive chromosomal remodeling and distinct sex chromosome systems (SCSs). So far, three different SCSs with male heterogamety have been characterized in the group. In some species, the presence of the XX/XY1Y2 SCS is associated with a decrease...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.877522/full |
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| author | Geize Aparecida Deon Geize Aparecida Deon Larissa Glugoski Larissa Glugoski Francisco de Menezes Cavalcante Sassi Terumi Hatanaka Viviane Nogaroto Luiz Antônio Carlos Bertollo Thomas Liehr Ahmed Al-Rikabi Orlando Moreira-Filho Marcelo de Bello Cioffi Marcelo Ricardo Vicari |
| author_facet | Geize Aparecida Deon Geize Aparecida Deon Larissa Glugoski Larissa Glugoski Francisco de Menezes Cavalcante Sassi Terumi Hatanaka Viviane Nogaroto Luiz Antônio Carlos Bertollo Thomas Liehr Ahmed Al-Rikabi Orlando Moreira-Filho Marcelo de Bello Cioffi Marcelo Ricardo Vicari |
| author_sort | Geize Aparecida Deon |
| collection | DOAJ |
| description | The Neotropical genus Harttia comprises species with extensive chromosomal remodeling and distinct sex chromosome systems (SCSs). So far, three different SCSs with male heterogamety have been characterized in the group. In some species, the presence of the XX/XY1Y2 SCS is associated with a decrease in diploid numbers and several chromosomal rearrangements, although a direct relation to sex chromosome differentiation has not been shown yet. Here, we aimed to investigate the differentiation processes that have led to the establishment of the rare XX/XY1Y2 SCS and track its evolutionary history among other Harttia species. For that, four whole chromosome painting probes derived from chromosome 1 of H. torrenticola (HTO-1), chromosomes 9 and X of H. carvalhoi (HCA-9 and HCA-X), and chromosome X from H. intermontana (HIN-X) were applied in nine Harttia species. Homeologous chromosome blocks were located in Harttia species and demonstrated that Robertsonian (Rb) fusions originated HTO-1, HCA-9, and HCA-X chromosomes, while Rb fissions explain Y1 and Y2 sex chromosomes. Specifically, in H. intermontana, HCA-X, HCA-9, and the NOR-bearing chromosome demonstrated that homeologous blocks were used in the HIN-X and metacentric pair 2 origins. Consequently, diploid numbers changed between the studied species. Overall, the data also reinforce the existence of unstable genomic sites promoting chromosomal differentiation and remodeling within the genus Harttia. |
| first_indexed | 2024-12-13T20:25:30Z |
| format | Article |
| id | doaj.art-9ec1477c691a438ea4fd1fd1c0c4ba24 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T20:25:30Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-9ec1477c691a438ea4fd1fd1c0c4ba242022-12-21T23:32:35ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-03-011310.3389/fgene.2022.877522877522Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae)Geize Aparecida Deon0Geize Aparecida Deon1Larissa Glugoski2Larissa Glugoski3Francisco de Menezes Cavalcante Sassi4Terumi Hatanaka5Viviane Nogaroto6Luiz Antônio Carlos Bertollo7Thomas Liehr8Ahmed Al-Rikabi9Orlando Moreira-Filho10Marcelo de Bello Cioffi11Marcelo Ricardo Vicari12Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilDepartamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Paraná, BrazilDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilDepartamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Paraná, BrazilDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilDepartamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Paraná, BrazilDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilInstitute of Human Genetics, University Hospital Jena, Jena, GermanyInstitute of Human Genetics, University Hospital Jena, Jena, GermanyDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilDepartamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, BrazilDepartamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Paraná, BrazilThe Neotropical genus Harttia comprises species with extensive chromosomal remodeling and distinct sex chromosome systems (SCSs). So far, three different SCSs with male heterogamety have been characterized in the group. In some species, the presence of the XX/XY1Y2 SCS is associated with a decrease in diploid numbers and several chromosomal rearrangements, although a direct relation to sex chromosome differentiation has not been shown yet. Here, we aimed to investigate the differentiation processes that have led to the establishment of the rare XX/XY1Y2 SCS and track its evolutionary history among other Harttia species. For that, four whole chromosome painting probes derived from chromosome 1 of H. torrenticola (HTO-1), chromosomes 9 and X of H. carvalhoi (HCA-9 and HCA-X), and chromosome X from H. intermontana (HIN-X) were applied in nine Harttia species. Homeologous chromosome blocks were located in Harttia species and demonstrated that Robertsonian (Rb) fusions originated HTO-1, HCA-9, and HCA-X chromosomes, while Rb fissions explain Y1 and Y2 sex chromosomes. Specifically, in H. intermontana, HCA-X, HCA-9, and the NOR-bearing chromosome demonstrated that homeologous blocks were used in the HIN-X and metacentric pair 2 origins. Consequently, diploid numbers changed between the studied species. Overall, the data also reinforce the existence of unstable genomic sites promoting chromosomal differentiation and remodeling within the genus Harttia.https://www.frontiersin.org/articles/10.3389/fgene.2022.877522/fullfishkaryotype evolutionmolecular cytogeneticsunstable genomic siteswhole chromosome painting |
| spellingShingle | Geize Aparecida Deon Geize Aparecida Deon Larissa Glugoski Larissa Glugoski Francisco de Menezes Cavalcante Sassi Terumi Hatanaka Viviane Nogaroto Luiz Antônio Carlos Bertollo Thomas Liehr Ahmed Al-Rikabi Orlando Moreira-Filho Marcelo de Bello Cioffi Marcelo Ricardo Vicari Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae) Frontiers in Genetics fish karyotype evolution molecular cytogenetics unstable genomic sites whole chromosome painting |
| title | Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae) |
| title_full | Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae) |
| title_fullStr | Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae) |
| title_full_unstemmed | Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae) |
| title_short | Chromosomal Rearrangements and Origin of the Multiple XX/XY1Y2 Sex Chromosome System in Harttia Species (Siluriformes: Loricariidae) |
| title_sort | chromosomal rearrangements and origin of the multiple xx xy1y2 sex chromosome system in harttia species siluriformes loricariidae |
| topic | fish karyotype evolution molecular cytogenetics unstable genomic sites whole chromosome painting |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.877522/full |
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