High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations
Abstract Background Leaf size and shape, which affect light capture, and chlorophyll content are important factors affecting photosynthetic efficiency. Genetic variation of these components significantly affects yield potential and seed quality. Identification of the genetic basis for these traits a...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02684-x |
| _version_ | 1819194848755843072 |
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| author | Kaiye Yu Jinshe Wang Chongyuan Sun Xiaoqian Liu Huanqing Xu Yuming Yang Lidong Dong Dan Zhang |
| author_facet | Kaiye Yu Jinshe Wang Chongyuan Sun Xiaoqian Liu Huanqing Xu Yuming Yang Lidong Dong Dan Zhang |
| author_sort | Kaiye Yu |
| collection | DOAJ |
| description | Abstract Background Leaf size and shape, which affect light capture, and chlorophyll content are important factors affecting photosynthetic efficiency. Genetic variation of these components significantly affects yield potential and seed quality. Identification of the genetic basis for these traits and the relationship between them is of great practical significance for achieving ideal plant architecture and high photosynthetic efficiency for improved yield. Results Here, we undertook a large-scale linkage mapping study using three mapping populations to determine the genetic interplay between soybean leaf-related traits and chlorophyll content across two environments. Correlation analysis revealed a significant negative correlation between leaf size and shape, while both traits were positively correlated with chlorophyll content. This phenotypic relationship was verified across the three mapping populations as determined by principal component analysis, suggesting that these traits are under the control of complex and interrelated genetic components. The QTLs for leaf-related traits and chlorophyll are partly shared, which further supports the close genetic relationship between the two traits. The largest-effect major loci, q20, was stably identified across all population and environments and harbored the narrow leaflet gene Gm-JAG1 (Ln/ln), which is a key regulator of leaflet shape in soybean. Conclusion Our results uncover several major QTLs (q4–1, q4–2, q11, q13, q18 and q20) and its candidate genes specific or common to leaf-related traits and chlorophyll, and also show a complex epistatic interaction between the two traits. The SNP markers closely linked to these valuable QTLs could be used for molecular design breeding with improved plant architecture, photosynthetic capacity and even yield. |
| first_indexed | 2024-12-23T02:03:23Z |
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| id | doaj.art-9ec19ff544c74b1c8c6967d28fe19760 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-23T02:03:23Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-9ec19ff544c74b1c8c6967d28fe197602022-12-21T18:03:56ZengBMCBMC Plant Biology1471-22292020-10-0120111410.1186/s12870-020-02684-xHigh-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populationsKaiye Yu0Jinshe Wang1Chongyuan Sun2Xiaoqian Liu3Huanqing Xu4Yuming Yang5Lidong Dong6Dan Zhang7National Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural UniversityZhengzhou National Subcenter for Soybean Improvement, Henan Academy of Agricultural SciencesNational Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural UniversityNational Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural UniversitySchool of Life Sciences, Guangzhou UniversityNational Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural UniversityAbstract Background Leaf size and shape, which affect light capture, and chlorophyll content are important factors affecting photosynthetic efficiency. Genetic variation of these components significantly affects yield potential and seed quality. Identification of the genetic basis for these traits and the relationship between them is of great practical significance for achieving ideal plant architecture and high photosynthetic efficiency for improved yield. Results Here, we undertook a large-scale linkage mapping study using three mapping populations to determine the genetic interplay between soybean leaf-related traits and chlorophyll content across two environments. Correlation analysis revealed a significant negative correlation between leaf size and shape, while both traits were positively correlated with chlorophyll content. This phenotypic relationship was verified across the three mapping populations as determined by principal component analysis, suggesting that these traits are under the control of complex and interrelated genetic components. The QTLs for leaf-related traits and chlorophyll are partly shared, which further supports the close genetic relationship between the two traits. The largest-effect major loci, q20, was stably identified across all population and environments and harbored the narrow leaflet gene Gm-JAG1 (Ln/ln), which is a key regulator of leaflet shape in soybean. Conclusion Our results uncover several major QTLs (q4–1, q4–2, q11, q13, q18 and q20) and its candidate genes specific or common to leaf-related traits and chlorophyll, and also show a complex epistatic interaction between the two traits. The SNP markers closely linked to these valuable QTLs could be used for molecular design breeding with improved plant architecture, photosynthetic capacity and even yield.http://link.springer.com/article/10.1186/s12870-020-02684-xSoybeanLeaves related traitsChlorophyll contentQuantitative trait lociGenetic relationship |
| spellingShingle | Kaiye Yu Jinshe Wang Chongyuan Sun Xiaoqian Liu Huanqing Xu Yuming Yang Lidong Dong Dan Zhang High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations BMC Plant Biology Soybean Leaves related traits Chlorophyll content Quantitative trait loci Genetic relationship |
| title | High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations |
| title_full | High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations |
| title_fullStr | High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations |
| title_full_unstemmed | High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations |
| title_short | High-density QTL mapping of leaf-related traits and chlorophyll content in three soybean RIL populations |
| title_sort | high density qtl mapping of leaf related traits and chlorophyll content in three soybean ril populations |
| topic | Soybean Leaves related traits Chlorophyll content Quantitative trait loci Genetic relationship |
| url | http://link.springer.com/article/10.1186/s12870-020-02684-x |
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