MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA
This study was aimed at isolating and identifying Salmonella sp. and then conducting an antibiotics susceptibility test in order to detect resistant genes. One hundred and five chicken cloaca swab samples were used in this study. 30 samples were taken from a layer farm in Bogor, 45 from a broiler f...
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| Format: | Article |
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Universitas Syiah Kuala
2022-04-01
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| Series: | Jurnal Kedokteran Hewan |
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| Online Access: | https://jurnal.usk.ac.id/JKH/article/view/18944 |
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| author | Aprilia Hardiati Safika Safika Fachriyan Hasmi Pasaribu I Wayan Teguh Wibawan |
| author_facet | Aprilia Hardiati Safika Safika Fachriyan Hasmi Pasaribu I Wayan Teguh Wibawan |
| author_sort | Aprilia Hardiati |
| collection | DOAJ |
| description | This study was aimed at isolating and identifying Salmonella sp. and then conducting an antibiotics susceptibility test in order to detect resistant genes. One hundred and five chicken cloaca swab samples were used in this study. 30 samples were taken from a layer farm in Bogor, 45 from a broiler farm in Sukabumi and 30 from a broiler farm in Cianjur. In order to isolate and identify the bacteria, a tetrathionate broth was used, which was then cultured in a Salmonella-Shigella agar, and finally a Gram stain and biochemical test was conducted. To confirm the presence of Salmonella sp., a pair of primers were used for the polymerase chain reaction (PCR) method to determine the presence of the invA gene.. An antibiotics susceptibility test was used with the Kirby-Bauer disk diffusion method. Nine antibiotics were used in this study. Each primer pair was used for the detection of tetA, blaTEM, aac(3)-IV, gyrA and ermB genes, and for genes encoding antibiotic resistance a PCR test was used. Eight (7.6%) Salmonella sp. were isolated in this study. All isolates showed positive results with PCR confirmation. The results of the antibiotics susceptibility test showed that Salmonella sp. isolates were resistant to tetracycline (75%), oxytetracycline (75%), amphicillin (75%), gentamycin (12.5%), nalidixic acid (100%), ciprofloxacin (12.5%), enrofloxacin (0%), erythromycin (100%), and chloramphenicol (0%). The distribution of antibiotic resistance genes in Salmonella sp. were tetA (33.3%), blaTEM (100%), aac(3)-IV (0%), gyrA (100%) and ermB (0%) positive. In conclusion, Salmonella sp. was isolated. All isolates showed positive results in the PCR confirmation. Salmonella sp. isolates were resistant to tetracycline, oxytetracycline, amphicillin, gentamycin, nalidixic acid, ciprofloxacin, and erythromycin. Only the tetA, blaTEM, and gyrA genes were detected in Salmonella sp. isolates. |
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| issn | 1978-225X 2502-5600 |
| language | English |
| last_indexed | 2025-03-22T04:17:56Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-9ec39d097fbe4342b2effbda4ec5ac0b2024-04-28T08:03:38ZengUniversitas Syiah KualaJurnal Kedokteran Hewan1978-225X2502-56002022-04-0116161110.21157/j.ked.hewan.v16i1.1894414110MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIAAprilia Hardiati0Safika Safika1Fachriyan Hasmi Pasaribu2I Wayan Teguh Wibawan3Medical Microbiology Division, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, IndonesiaMedical Microbiology Division, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, IndonesiaMedical Microbiology Division, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, IndonesiaMedical Microbiology Division, Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, IndonesiaThis study was aimed at isolating and identifying Salmonella sp. and then conducting an antibiotics susceptibility test in order to detect resistant genes. One hundred and five chicken cloaca swab samples were used in this study. 30 samples were taken from a layer farm in Bogor, 45 from a broiler farm in Sukabumi and 30 from a broiler farm in Cianjur. In order to isolate and identify the bacteria, a tetrathionate broth was used, which was then cultured in a Salmonella-Shigella agar, and finally a Gram stain and biochemical test was conducted. To confirm the presence of Salmonella sp., a pair of primers were used for the polymerase chain reaction (PCR) method to determine the presence of the invA gene.. An antibiotics susceptibility test was used with the Kirby-Bauer disk diffusion method. Nine antibiotics were used in this study. Each primer pair was used for the detection of tetA, blaTEM, aac(3)-IV, gyrA and ermB genes, and for genes encoding antibiotic resistance a PCR test was used. Eight (7.6%) Salmonella sp. were isolated in this study. All isolates showed positive results with PCR confirmation. The results of the antibiotics susceptibility test showed that Salmonella sp. isolates were resistant to tetracycline (75%), oxytetracycline (75%), amphicillin (75%), gentamycin (12.5%), nalidixic acid (100%), ciprofloxacin (12.5%), enrofloxacin (0%), erythromycin (100%), and chloramphenicol (0%). The distribution of antibiotic resistance genes in Salmonella sp. were tetA (33.3%), blaTEM (100%), aac(3)-IV (0%), gyrA (100%) and ermB (0%) positive. In conclusion, Salmonella sp. was isolated. All isolates showed positive results in the PCR confirmation. Salmonella sp. isolates were resistant to tetracycline, oxytetracycline, amphicillin, gentamycin, nalidixic acid, ciprofloxacin, and erythromycin. Only the tetA, blaTEM, and gyrA genes were detected in Salmonella sp. isolates.https://jurnal.usk.ac.id/JKH/article/view/18944antibiotics, chicken, resistance gene, salmonella sp. |
| spellingShingle | Aprilia Hardiati Safika Safika Fachriyan Hasmi Pasaribu I Wayan Teguh Wibawan MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA Jurnal Kedokteran Hewan antibiotics, chicken, resistance gene, salmonella sp. |
| title | MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA |
| title_full | MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA |
| title_fullStr | MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA |
| title_full_unstemmed | MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA |
| title_short | MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA |
| title_sort | multidrug resistant salmonella sp isolated from several chicken farms in west java indonesia |
| topic | antibiotics, chicken, resistance gene, salmonella sp. |
| url | https://jurnal.usk.ac.id/JKH/article/view/18944 |
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