Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze
Tea (<i>Camellia sinensis</i>) is one of the most important cash crops in the world. Theanine, as an important amino acid component in tea, is a key quality index for excellent tea quality and high economic value. People increase theanine accumulation in tea mainly through the applicatio...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3290 |
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| author | Wei Li Fen Xiang Yi Su Zhoufei Luo Weigui Luo Lingyun Zhou Hongyan Liu Langtao Xiao |
| author_facet | Wei Li Fen Xiang Yi Su Zhoufei Luo Weigui Luo Lingyun Zhou Hongyan Liu Langtao Xiao |
| author_sort | Wei Li |
| collection | DOAJ |
| description | Tea (<i>Camellia sinensis</i>) is one of the most important cash crops in the world. Theanine, as an important amino acid component in tea, is a key quality index for excellent tea quality and high economic value. People increase theanine accumulation in tea mainly through the application of nitrogen fertilizer, shading and pruning. However, these methods are not effective. In this study, we treated tea buds with a 100 μM solution of GA<sub>3</sub> containing 1‰ tween-20, investigated the effects of GA<sub>3</sub> on theanine accumulation, bud yield, chlorophyll fluorescence parameters and expression level of theanine biosynthesis pathway genes in tea plant by qPCR, LC-MS/MS etc. Results showed that change trends of theanine and GA<sub>3</sub> was extremely positively correlated with each other. Exogenous GA<sub>3</sub> upregulated the expression level of theanine biosynthesis pathway genes, caused an increase of theanine content (mg·g<sup>-1</sup>) by 27% in tea leaves compared with Mock, and accelerated the germination of buds and elongation of shoots, which lead to a significant increase of tea yield by 56% (<i>w</i>/<i>w</i>). Moreover, the decrease of chlorophyll contents, photochemical quenching coefficient (<i>qP</i>) and relative electron transport rate (<i>rETR</i>) under GA<sub>3</sub> treatment suggested that GA<sub>3</sub> reduced photosynthesis in the tender tea leaves, indicating that the decline of carbon assimilation in tea plants was conducive to the nitrogen metabolism, and it was beneficial to the accumulation of theanine. This study provided a new technical and theoretical support for the precise control of tea quality components and phenophase. |
| first_indexed | 2024-03-10T10:53:51Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:53:51Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-9ece5cbdb764410bb8dd388b6415d4202023-11-21T22:01:39ZengMDPI AGMolecules1420-30492021-05-012611329010.3390/molecules26113290Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) KuntzeWei Li0Fen Xiang1Yi Su2Zhoufei Luo3Weigui Luo4Lingyun Zhou5Hongyan Liu6Langtao Xiao7Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410125, ChinaTea Research Institute, Hunan Academy of Agricultural Science, Changsha 410125, ChinaHunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410125, ChinaHunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410125, ChinaHunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410125, ChinaTea Research Institute, Hunan Academy of Agricultural Science, Changsha 410125, ChinaTea Research Institute, Hunan Academy of Agricultural Science, Changsha 410125, ChinaHunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410125, ChinaTea (<i>Camellia sinensis</i>) is one of the most important cash crops in the world. Theanine, as an important amino acid component in tea, is a key quality index for excellent tea quality and high economic value. People increase theanine accumulation in tea mainly through the application of nitrogen fertilizer, shading and pruning. However, these methods are not effective. In this study, we treated tea buds with a 100 μM solution of GA<sub>3</sub> containing 1‰ tween-20, investigated the effects of GA<sub>3</sub> on theanine accumulation, bud yield, chlorophyll fluorescence parameters and expression level of theanine biosynthesis pathway genes in tea plant by qPCR, LC-MS/MS etc. Results showed that change trends of theanine and GA<sub>3</sub> was extremely positively correlated with each other. Exogenous GA<sub>3</sub> upregulated the expression level of theanine biosynthesis pathway genes, caused an increase of theanine content (mg·g<sup>-1</sup>) by 27% in tea leaves compared with Mock, and accelerated the germination of buds and elongation of shoots, which lead to a significant increase of tea yield by 56% (<i>w</i>/<i>w</i>). Moreover, the decrease of chlorophyll contents, photochemical quenching coefficient (<i>qP</i>) and relative electron transport rate (<i>rETR</i>) under GA<sub>3</sub> treatment suggested that GA<sub>3</sub> reduced photosynthesis in the tender tea leaves, indicating that the decline of carbon assimilation in tea plants was conducive to the nitrogen metabolism, and it was beneficial to the accumulation of theanine. This study provided a new technical and theoretical support for the precise control of tea quality components and phenophase.https://www.mdpi.com/1420-3049/26/11/3290<i>Camellia sinensis</i>theaninegibberellinchlorophyll fluorescence |
| spellingShingle | Wei Li Fen Xiang Yi Su Zhoufei Luo Weigui Luo Lingyun Zhou Hongyan Liu Langtao Xiao Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze Molecules <i>Camellia sinensis</i> theanine gibberellin chlorophyll fluorescence |
| title | Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze |
| title_full | Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze |
| title_fullStr | Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze |
| title_full_unstemmed | Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze |
| title_short | Gibberellin Increases the Bud Yield and Theanine Accumulation in <i>Camellia sinensis</i> (L.) Kuntze |
| title_sort | gibberellin increases the bud yield and theanine accumulation in i camellia sinensis i l kuntze |
| topic | <i>Camellia sinensis</i> theanine gibberellin chlorophyll fluorescence |
| url | https://www.mdpi.com/1420-3049/26/11/3290 |
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