Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems
Upland forest ecosystems are recognized as net sinks for atmospheric methane (CH<sub>4</sub>), one of the most impactful greenhouse gases. Biological methane uptake in these ecosystems occurs due to the activity of aerobic methanotrophic bacteria. Russia hosts one-fifth of the global for...
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MDPI AG
2021-12-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/12/1738 |
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| author | Aleksandr F. Sabrekov Olga V. Danilova Irina E. Terentieva Anastasia A. Ivanova Svetlana E. Belova Yuri V. Litti Mikhail V. Glagolev Svetlana N. Dedysh |
| author_facet | Aleksandr F. Sabrekov Olga V. Danilova Irina E. Terentieva Anastasia A. Ivanova Svetlana E. Belova Yuri V. Litti Mikhail V. Glagolev Svetlana N. Dedysh |
| author_sort | Aleksandr F. Sabrekov |
| collection | DOAJ |
| description | Upland forest ecosystems are recognized as net sinks for atmospheric methane (CH<sub>4</sub>), one of the most impactful greenhouse gases. Biological methane uptake in these ecosystems occurs due to the activity of aerobic methanotrophic bacteria. Russia hosts one-fifth of the global forest area, with the most extensive forest landscapes located in West Siberia. Here, we report seasonal CH<sub>4</sub> flux measurements conducted in 2018 in three types of stands in West Siberian middle taiga–Siberian pine, Aspen, and mixed forests. High rates of methane uptake of up to −0.184 mg CH<sub>4</sub> m<sup>−2</sup> h<sup>−1</sup> were measured by a static chamber method, with an estimated total growing season consumption of 4.5 ± 0.5 kg CH<sub>4</sub> ha<sup>−1</sup>. Forest type had little to no effect on methane fluxes within each season. Soil methane oxidation rate ranged from 0 to 8.1 ng CH<sub>4</sub> g<sub>DW</sub><sup>−1</sup> h<sup>−1</sup> and was negatively related to water-filled pore space. The microbial soil communities were dominated by the <i>Alpha</i>- and <i>Gammaproteobacteria</i>, <i>Acidobacteriota</i> and <i>Actinobacteriota</i>. The major group of 16S rRNA gene reads from methanotrophs belonged to uncultivated <i>Beijerinckiaceae</i> bacteria. Molecular identification of methanotrophs based on retrieval of the <i>pmoA</i> gene confirmed that Upland Soil Cluster Alpha was the major bacterial group responsible for CH<sub>4</sub> oxidation. |
| first_indexed | 2024-03-10T04:06:26Z |
| format | Article |
| id | doaj.art-9ed175f331554a2fbefc0d3871492d02 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T04:06:26Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Forests |
| spelling | doaj.art-9ed175f331554a2fbefc0d3871492d022023-11-23T08:21:43ZengMDPI AGForests1999-49072021-12-011212173810.3390/f12121738Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest EcosystemsAleksandr F. Sabrekov0Olga V. Danilova1Irina E. Terentieva2Anastasia A. Ivanova3Svetlana E. Belova4Yuri V. Litti5Mikhail V. Glagolev6Svetlana N. Dedysh7A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, RussiaA.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaUpland forest ecosystems are recognized as net sinks for atmospheric methane (CH<sub>4</sub>), one of the most impactful greenhouse gases. Biological methane uptake in these ecosystems occurs due to the activity of aerobic methanotrophic bacteria. Russia hosts one-fifth of the global forest area, with the most extensive forest landscapes located in West Siberia. Here, we report seasonal CH<sub>4</sub> flux measurements conducted in 2018 in three types of stands in West Siberian middle taiga–Siberian pine, Aspen, and mixed forests. High rates of methane uptake of up to −0.184 mg CH<sub>4</sub> m<sup>−2</sup> h<sup>−1</sup> were measured by a static chamber method, with an estimated total growing season consumption of 4.5 ± 0.5 kg CH<sub>4</sub> ha<sup>−1</sup>. Forest type had little to no effect on methane fluxes within each season. Soil methane oxidation rate ranged from 0 to 8.1 ng CH<sub>4</sub> g<sub>DW</sub><sup>−1</sup> h<sup>−1</sup> and was negatively related to water-filled pore space. The microbial soil communities were dominated by the <i>Alpha</i>- and <i>Gammaproteobacteria</i>, <i>Acidobacteriota</i> and <i>Actinobacteriota</i>. The major group of 16S rRNA gene reads from methanotrophs belonged to uncultivated <i>Beijerinckiaceae</i> bacteria. Molecular identification of methanotrophs based on retrieval of the <i>pmoA</i> gene confirmed that Upland Soil Cluster Alpha was the major bacterial group responsible for CH<sub>4</sub> oxidation.https://www.mdpi.com/1999-4907/12/12/1738atmospheric methane oxidationmethane fluxesboreal forestsupland soilsbacterial diversitymethanotrophic bacteria |
| spellingShingle | Aleksandr F. Sabrekov Olga V. Danilova Irina E. Terentieva Anastasia A. Ivanova Svetlana E. Belova Yuri V. Litti Mikhail V. Glagolev Svetlana N. Dedysh Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems Forests atmospheric methane oxidation methane fluxes boreal forests upland soils bacterial diversity methanotrophic bacteria |
| title | Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems |
| title_full | Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems |
| title_fullStr | Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems |
| title_full_unstemmed | Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems |
| title_short | Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems |
| title_sort | atmospheric methane consumption and methanotroph communities in west siberian boreal upland forest ecosystems |
| topic | atmospheric methane oxidation methane fluxes boreal forests upland soils bacterial diversity methanotrophic bacteria |
| url | https://www.mdpi.com/1999-4907/12/12/1738 |
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