Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible
Abstract In vitro studies of implant-tissue attachment are primarily based on two-dimensional cell culture models, which fail to replicate the three-dimensional native human oral mucosal tissue completely. Thus, the present study aimed to describe a novel tissue culture model using pig mandibular bl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2021-10-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-021-06608-5 |
| _version_ | 1818730094974205952 |
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| author | Nagat Areid Jaana Willberg Ilkka Kangasniemi Timo O. Närhi |
| author_facet | Nagat Areid Jaana Willberg Ilkka Kangasniemi Timo O. Närhi |
| author_sort | Nagat Areid |
| collection | DOAJ |
| description | Abstract In vitro studies of implant-tissue attachment are primarily based on two-dimensional cell culture models, which fail to replicate the three-dimensional native human oral mucosal tissue completely. Thus, the present study aimed to describe a novel tissue culture model using pig mandibular block including alveolar bone and gingival soft tissues to evaluate the tissue attachment to titanium implant provided with hydrothermally induced TiO2 coating. Tissue attachment on TiO2 coated and non-coated implants were compared. Ti-6Al-4V alloy posts were used to function as implants that were inserted in five pig mandibles. Implants were delivered with two different surface treatments, non-coated (NC) titanium and hydrothermal induced TiO2 coated surfaces (HT). The tissue-implant specimens were cultured at an air/liquid interface for 7 and 14 days. The tissue-implant interface was analyzed by histological and immunohistochemical stainings. The microscopic evaluation suggests that pig tissue explants established soft and hard tissue attachment to both implant surfaces. The epithelial cells appeared to attach to the coated implant. The epithelium adjacent to the implant abutment starts to change its phenotype during the early days of the healing process. New bone formation was seen within small pieces of bone in close contact with the coated implant. In conclusion, this in vitro model maintains the viability of pig tissue and allows histologically and immunohistochemically evaluate the tissue-implant interface. HT-induced TiO2 coating seems to have a favorable tissue response. Moreover, this organotypic tissue culture model is applicable for further studies with quantitative parameters to evaluate adhesion molecules present at the implant-tissue interface. |
| first_indexed | 2024-12-17T22:56:20Z |
| format | Article |
| id | doaj.art-9ed623ee9cf2457885bd285f8bf13ad6 |
| institution | Directory Open Access Journal |
| issn | 0957-4530 1573-4838 |
| language | English |
| last_indexed | 2024-12-17T22:56:20Z |
| publishDate | 2021-10-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj.art-9ed623ee9cf2457885bd285f8bf13ad62022-12-21T21:29:33ZengSpringerJournal of Materials Science: Materials in Medicine0957-45301573-48382021-10-01321111010.1007/s10856-021-06608-5Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandibleNagat Areid0Jaana Willberg1Ilkka Kangasniemi2Timo O. Närhi3Department of Prosthetic Dentistry and Stomatognathic Physiology, Institute of Dentistry, University of TurkuDepartment of Oral Pathology and Radiology, Institute of Dentistry, University of TurkuTurku Clinical Biomaterials Centre (TCBC), University of TurkuDepartment of Prosthetic Dentistry and Stomatognathic Physiology, Institute of Dentistry, University of TurkuAbstract In vitro studies of implant-tissue attachment are primarily based on two-dimensional cell culture models, which fail to replicate the three-dimensional native human oral mucosal tissue completely. Thus, the present study aimed to describe a novel tissue culture model using pig mandibular block including alveolar bone and gingival soft tissues to evaluate the tissue attachment to titanium implant provided with hydrothermally induced TiO2 coating. Tissue attachment on TiO2 coated and non-coated implants were compared. Ti-6Al-4V alloy posts were used to function as implants that were inserted in five pig mandibles. Implants were delivered with two different surface treatments, non-coated (NC) titanium and hydrothermal induced TiO2 coated surfaces (HT). The tissue-implant specimens were cultured at an air/liquid interface for 7 and 14 days. The tissue-implant interface was analyzed by histological and immunohistochemical stainings. The microscopic evaluation suggests that pig tissue explants established soft and hard tissue attachment to both implant surfaces. The epithelial cells appeared to attach to the coated implant. The epithelium adjacent to the implant abutment starts to change its phenotype during the early days of the healing process. New bone formation was seen within small pieces of bone in close contact with the coated implant. In conclusion, this in vitro model maintains the viability of pig tissue and allows histologically and immunohistochemically evaluate the tissue-implant interface. HT-induced TiO2 coating seems to have a favorable tissue response. Moreover, this organotypic tissue culture model is applicable for further studies with quantitative parameters to evaluate adhesion molecules present at the implant-tissue interface.https://doi.org/10.1007/s10856-021-06608-5 |
| spellingShingle | Nagat Areid Jaana Willberg Ilkka Kangasniemi Timo O. Närhi Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible Journal of Materials Science: Materials in Medicine |
| title | Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible |
| title_full | Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible |
| title_fullStr | Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible |
| title_full_unstemmed | Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible |
| title_short | Organotypic in vitro block culture model to investigate tissue-implant interface. An experimental study on pig mandible |
| title_sort | organotypic in vitro block culture model to investigate tissue implant interface an experimental study on pig mandible |
| url | https://doi.org/10.1007/s10856-021-06608-5 |
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