The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio
Pseudomonas aeruginosa is a common gram-negative pathogen belonging to the Aeromonas genus. In the present study, dominant bacteria were isolated from diseased crucian carp (Carassius auratus gibelio). The isolate was identified as P. aeruginosa based on biochemical identification and 16S rDNA seque...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422001223 |
| _version_ | 1818534594224324608 |
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| author | Tongwei Ji Yangyang Cao Qingsheng Cao Yingying Zhang Hui Yang |
| author_facet | Tongwei Ji Yangyang Cao Qingsheng Cao Yingying Zhang Hui Yang |
| author_sort | Tongwei Ji |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a common gram-negative pathogen belonging to the Aeromonas genus. In the present study, dominant bacteria were isolated from diseased crucian carp (Carassius auratus gibelio). The isolate was identified as P. aeruginosa based on biochemical identification and 16S rDNA sequencing. In the histological observation of diseased C. auratus, a large number of vacuoles and inflammatory infiltration appeared in the liver, the structure of intestinal villi was loose, and a large number of inflammatory cells and vacuoles were found in gill. Five virulence genes exoU, exoS, pcrV, exoT and exoY were identified in this P. aeruginosa isolate. Drug sensitivity testing showed that the isolate was susceptible to norfloxacin, ofloxacin and ciprofloxacin. The supernatant of Bacillus coagulans NJ3 exhibited a significant bactericidal effect on isolated P. aeruginosa based on the inhibition zone experiment. The accumulative mortality of C. auratus caused by P. aeruginosa infection could be significantly reduced by adding B. coagulans NJ3 into the aquaculture water. These results indicated that B. coagulans NJ3 could be used as probiotics to prevent the infections of P. aeruginosa in C. auratus. The present study provided a scientific basis for the identification of P. aeruginosa, and treatment for this pathogen infections in fish by using B. coagulans NJ3. |
| first_indexed | 2024-12-11T18:13:36Z |
| format | Article |
| id | doaj.art-9edb348f019341a7b0b25985dbaa8e1b |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-11T18:13:36Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj.art-9edb348f019341a7b0b25985dbaa8e1b2022-12-22T00:55:29ZengElsevierAquaculture Reports2352-51342022-06-0124101126The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelioTongwei Ji0Yangyang Cao1Qingsheng Cao2Yingying Zhang3Hui Yang4College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCorrespondence to: College of Animal Science and Technology, Yangzhou University, 48 Wenhui Road, Yangzhou, Jiangsu 225009 China.; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaPseudomonas aeruginosa is a common gram-negative pathogen belonging to the Aeromonas genus. In the present study, dominant bacteria were isolated from diseased crucian carp (Carassius auratus gibelio). The isolate was identified as P. aeruginosa based on biochemical identification and 16S rDNA sequencing. In the histological observation of diseased C. auratus, a large number of vacuoles and inflammatory infiltration appeared in the liver, the structure of intestinal villi was loose, and a large number of inflammatory cells and vacuoles were found in gill. Five virulence genes exoU, exoS, pcrV, exoT and exoY were identified in this P. aeruginosa isolate. Drug sensitivity testing showed that the isolate was susceptible to norfloxacin, ofloxacin and ciprofloxacin. The supernatant of Bacillus coagulans NJ3 exhibited a significant bactericidal effect on isolated P. aeruginosa based on the inhibition zone experiment. The accumulative mortality of C. auratus caused by P. aeruginosa infection could be significantly reduced by adding B. coagulans NJ3 into the aquaculture water. These results indicated that B. coagulans NJ3 could be used as probiotics to prevent the infections of P. aeruginosa in C. auratus. The present study provided a scientific basis for the identification of P. aeruginosa, and treatment for this pathogen infections in fish by using B. coagulans NJ3.http://www.sciencedirect.com/science/article/pii/S2352513422001223Pseudomonas aeruginosaBacillus coagulansAntagonistic effectAccumulative mortalityVirulence genes |
| spellingShingle | Tongwei Ji Yangyang Cao Qingsheng Cao Yingying Zhang Hui Yang The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio Aquaculture Reports Pseudomonas aeruginosa Bacillus coagulans Antagonistic effect Accumulative mortality Virulence genes |
| title | The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio |
| title_full | The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio |
| title_fullStr | The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio |
| title_full_unstemmed | The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio |
| title_short | The antagonistic effect and protective efficacy of gram-positive probiotics Bacillus coagulans to newly identified pathogens Pseudomonas aeruginosa in crucian carp Carassius auratus gibelio |
| title_sort | antagonistic effect and protective efficacy of gram positive probiotics bacillus coagulans to newly identified pathogens pseudomonas aeruginosa in crucian carp carassius auratus gibelio |
| topic | Pseudomonas aeruginosa Bacillus coagulans Antagonistic effect Accumulative mortality Virulence genes |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422001223 |
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