A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome
<p>Abstract</p> <p>Background</p> <p><it>Arachis hypogaea </it>(peanut) is an important crop worldwide, being mostly used for edible oil production, direct consumption and animal feed. Cultivated peanut is an allotetraploid species with two different genome...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-04-01
|
| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/9/40 |
| _version_ | 1811315052994625536 |
|---|---|
| author | Valls José FM Cavallari Marcelo M Lopes Catalina R Pereira Rinaldo W Guimarães Patrícia M Leal-Bertioli Soraya CM Teixeira Cristiane Alves-Freitas Dione MT Barbosa Andrea VG Moretzsohn Márcio C Bertioli David J Gimenes Marcos A |
| author_facet | Valls José FM Cavallari Marcelo M Lopes Catalina R Pereira Rinaldo W Guimarães Patrícia M Leal-Bertioli Soraya CM Teixeira Cristiane Alves-Freitas Dione MT Barbosa Andrea VG Moretzsohn Márcio C Bertioli David J Gimenes Marcos A |
| author_sort | Valls José FM |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Arachis hypogaea </it>(peanut) is an important crop worldwide, being mostly used for edible oil production, direct consumption and animal feed. Cultivated peanut is an allotetraploid species with two different genome components, A and B. Genetic linkage maps can greatly assist molecular breeding and genomic studies. However, the development of linkage maps for <it>A. hypogaea </it>is difficult because it has very low levels of polymorphism. This can be overcome by the utilization of wild species of <it>Arachis</it>, which present the A- and B-genomes in the diploid state, and show high levels of genetic variability.</p> <p>Results</p> <p>In this work, we constructed a B-genome linkage map, which will complement the previously published map for the A-genome of <it>Arachis</it>, and produced an entire framework for the tetraploid genome. This map is based on an F<sub>2 </sub>population of 93 individuals obtained from the cross between the diploid <it>A. ipaënsis </it>(K30076) and the closely related <it>A. magna </it>(K30097), the former species being the most probable B genome donor to cultivated peanut. In spite of being classified as different species, the parents showed high crossability and relatively low polymorphism (22.3%), compared to other interspecific crosses. The map has 10 linkage groups, with 149 loci spanning a total map distance of 1,294 cM. The microsatellite markers utilized, developed for other <it>Arachis </it>species, showed high transferability (81.7%). Segregation distortion was 21.5%. This B-genome map was compared to the A-genome map using 51 common markers, revealing a high degree of synteny between both genomes.</p> <p>Conclusion</p> <p>The development of genetic maps for <it>Arachis </it>diploid wild species with A- and B-genomes effectively provides a genetic map for the tetraploid cultivated peanut in two separate diploid components and is a significant advance towards the construction of a transferable reference map for <it>Arachis</it>. Additionally, we were able to identify affinities of some <it>Arachis </it>linkage groups with <it>Medicago truncatula</it>, which will allow the transfer of information from the nearly-complete genome sequences of this model legume to the peanut crop.</p> |
| first_indexed | 2024-04-13T11:23:23Z |
| format | Article |
| id | doaj.art-9edba97bc2424287b016a0a84e3c98db |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T11:23:23Z |
| publishDate | 2009-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-9edba97bc2424287b016a0a84e3c98db2022-12-22T02:48:46ZengBMCBMC Plant Biology1471-22292009-04-01914010.1186/1471-2229-9-40A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genomeValls José FMCavallari Marcelo MLopes Catalina RPereira Rinaldo WGuimarães Patrícia MLeal-Bertioli Soraya CMTeixeira CristianeAlves-Freitas Dione MTBarbosa Andrea VGMoretzsohn Márcio CBertioli David JGimenes Marcos A<p>Abstract</p> <p>Background</p> <p><it>Arachis hypogaea </it>(peanut) is an important crop worldwide, being mostly used for edible oil production, direct consumption and animal feed. Cultivated peanut is an allotetraploid species with two different genome components, A and B. Genetic linkage maps can greatly assist molecular breeding and genomic studies. However, the development of linkage maps for <it>A. hypogaea </it>is difficult because it has very low levels of polymorphism. This can be overcome by the utilization of wild species of <it>Arachis</it>, which present the A- and B-genomes in the diploid state, and show high levels of genetic variability.</p> <p>Results</p> <p>In this work, we constructed a B-genome linkage map, which will complement the previously published map for the A-genome of <it>Arachis</it>, and produced an entire framework for the tetraploid genome. This map is based on an F<sub>2 </sub>population of 93 individuals obtained from the cross between the diploid <it>A. ipaënsis </it>(K30076) and the closely related <it>A. magna </it>(K30097), the former species being the most probable B genome donor to cultivated peanut. In spite of being classified as different species, the parents showed high crossability and relatively low polymorphism (22.3%), compared to other interspecific crosses. The map has 10 linkage groups, with 149 loci spanning a total map distance of 1,294 cM. The microsatellite markers utilized, developed for other <it>Arachis </it>species, showed high transferability (81.7%). Segregation distortion was 21.5%. This B-genome map was compared to the A-genome map using 51 common markers, revealing a high degree of synteny between both genomes.</p> <p>Conclusion</p> <p>The development of genetic maps for <it>Arachis </it>diploid wild species with A- and B-genomes effectively provides a genetic map for the tetraploid cultivated peanut in two separate diploid components and is a significant advance towards the construction of a transferable reference map for <it>Arachis</it>. Additionally, we were able to identify affinities of some <it>Arachis </it>linkage groups with <it>Medicago truncatula</it>, which will allow the transfer of information from the nearly-complete genome sequences of this model legume to the peanut crop.</p>http://www.biomedcentral.com/1471-2229/9/40 |
| spellingShingle | Valls José FM Cavallari Marcelo M Lopes Catalina R Pereira Rinaldo W Guimarães Patrícia M Leal-Bertioli Soraya CM Teixeira Cristiane Alves-Freitas Dione MT Barbosa Andrea VG Moretzsohn Márcio C Bertioli David J Gimenes Marcos A A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome BMC Plant Biology |
| title | A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome |
| title_full | A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome |
| title_fullStr | A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome |
| title_full_unstemmed | A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome |
| title_short | A linkage map for the B-genome of <it>Arachis </it>(Fabaceae) and its synteny to the A-genome |
| title_sort | linkage map for the b genome of it arachis it fabaceae and its synteny to the a genome |
| url | http://www.biomedcentral.com/1471-2229/9/40 |
| work_keys_str_mv | AT vallsjosefm alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT cavallarimarcelom alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT lopescatalinar alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT pereirarinaldow alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT guimaraespatriciam alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT lealbertiolisorayacm alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT teixeiracristiane alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT alvesfreitasdionemt alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT barbosaandreavg alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT moretzsohnmarcioc alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT bertiolidavidj alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT gimenesmarcosa alinkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT vallsjosefm linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT cavallarimarcelom linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT lopescatalinar linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT pereirarinaldow linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT guimaraespatriciam linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT lealbertiolisorayacm linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT teixeiracristiane linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT alvesfreitasdionemt linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT barbosaandreavg linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT moretzsohnmarcioc linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT bertiolidavidj linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome AT gimenesmarcosa linkagemapforthebgenomeofitarachisitfabaceaeanditssyntenytotheagenome |