Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand
<i>Bartonella quintana</i> is a zoonotic pathogen with a worldwide distribution. Humans and non-human primates are considered to be natural reservoir hosts for <i>B. quintana.</i> However, information on the molecular epidemiology of this organism is very limited in regard to...
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MDPI AG
2021-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/5/629 |
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| author | Wanat Sricharern Supakarn Kaewchot Phirabhat Saengsawang Sarawan Kaewmongkol Tawin Inpankaew |
| author_facet | Wanat Sricharern Supakarn Kaewchot Phirabhat Saengsawang Sarawan Kaewmongkol Tawin Inpankaew |
| author_sort | Wanat Sricharern |
| collection | DOAJ |
| description | <i>Bartonella quintana</i> is a zoonotic pathogen with a worldwide distribution. Humans and non-human primates are considered to be natural reservoir hosts for <i>B. quintana.</i> However, information on the molecular epidemiology of this organism is very limited in regard to long-tailed macaques (<i>Macaca fascicularis</i>) in Thailand. Therefore, this study aimed to investigate the occurrence and genetic diversity of <i>Bartonella</i> spp. among long-tailed macaques in Thailand. In total, 856 blood samples were collected from long-tailed macaques in Thailand. All specimens were screened for <i>Bartonella</i> spp. using a polymerase chain reaction (PCR) assay targeting the 16S rRNA, <i>gltA</i> and <i>ftsZ</i> genes. All positive samples were further analyzed based on nucleotide sequencing, phylogenetic analysis and multiple sequence alignment analysis. Only one macaque showed a positive result in the PCR assays based on the 16S rRNA, <i>gltA</i> and <i>ftsZ</i> genes. Nucleotide sequencing and phylogenetic analysis revealed that the obtained sequences were closely related to <i>B. quintana</i> previously detected in non-human primates. Single-nucleotide polymorphisms (SNPs) were detected in the <i>gltA</i> and <i>ftsZ</i> gene sequences. This study revealed that long-tailed macaques in Thailand carried <i>B. quintana.</i> Despite the low infection rate detected, long-tailed macaques may be a reservoir of <i>B. quintana</i>. |
| first_indexed | 2024-03-10T11:15:22Z |
| format | Article |
| id | doaj.art-9ede827a207b4bc29d3af7f674a7a47f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T11:15:22Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-9ede827a207b4bc29d3af7f674a7a47f2023-11-21T20:29:17ZengMDPI AGPathogens2076-08172021-05-0110562910.3390/pathogens10050629Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in ThailandWanat Sricharern0Supakarn Kaewchot1Phirabhat Saengsawang2Sarawan Kaewmongkol3Tawin Inpankaew4Center for Agricultural Biotechnology, Kamphaengsaen Campus, Kasetsart University, Nakhonpathom 73140, ThailandDepartment of National Parks, Wildlife and Plant Conservation, Bangkok 10900, ThailandAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80161, ThailandDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, ThailandCenter for Agricultural Biotechnology, Kamphaengsaen Campus, Kasetsart University, Nakhonpathom 73140, Thailand<i>Bartonella quintana</i> is a zoonotic pathogen with a worldwide distribution. Humans and non-human primates are considered to be natural reservoir hosts for <i>B. quintana.</i> However, information on the molecular epidemiology of this organism is very limited in regard to long-tailed macaques (<i>Macaca fascicularis</i>) in Thailand. Therefore, this study aimed to investigate the occurrence and genetic diversity of <i>Bartonella</i> spp. among long-tailed macaques in Thailand. In total, 856 blood samples were collected from long-tailed macaques in Thailand. All specimens were screened for <i>Bartonella</i> spp. using a polymerase chain reaction (PCR) assay targeting the 16S rRNA, <i>gltA</i> and <i>ftsZ</i> genes. All positive samples were further analyzed based on nucleotide sequencing, phylogenetic analysis and multiple sequence alignment analysis. Only one macaque showed a positive result in the PCR assays based on the 16S rRNA, <i>gltA</i> and <i>ftsZ</i> genes. Nucleotide sequencing and phylogenetic analysis revealed that the obtained sequences were closely related to <i>B. quintana</i> previously detected in non-human primates. Single-nucleotide polymorphisms (SNPs) were detected in the <i>gltA</i> and <i>ftsZ</i> gene sequences. This study revealed that long-tailed macaques in Thailand carried <i>B. quintana.</i> Despite the low infection rate detected, long-tailed macaques may be a reservoir of <i>B. quintana</i>.https://www.mdpi.com/2076-0817/10/5/629<i>Bartonella quintana</i>long-tailed macaque<i>Macaca fascicularis</i>Thailand |
| spellingShingle | Wanat Sricharern Supakarn Kaewchot Phirabhat Saengsawang Sarawan Kaewmongkol Tawin Inpankaew Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand Pathogens <i>Bartonella quintana</i> long-tailed macaque <i>Macaca fascicularis</i> Thailand |
| title | Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand |
| title_full | Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand |
| title_fullStr | Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand |
| title_full_unstemmed | Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand |
| title_short | Molecular Detection of <i>Bartonella quintana</i> among Long-Tailed Macaques (<i>Macaca fascicularis</i>) in Thailand |
| title_sort | molecular detection of i bartonella quintana i among long tailed macaques i macaca fascicularis i in thailand |
| topic | <i>Bartonella quintana</i> long-tailed macaque <i>Macaca fascicularis</i> Thailand |
| url | https://www.mdpi.com/2076-0817/10/5/629 |
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