Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle
The study examined the polymorphism of the glutathione peroxidase-1 gene (GPX-1, g. 189 T/C) effect on the GPx enzyme activity and biochemical parameters in the blood serum of Holstein cows of indigenous and foreign selection. The work was carried out in the Lenin Breeding Farm Integrated Agricultur...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/99/e3sconf_afe23_01018.pdf |
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| author | Safina Natalia Shakirov Shamil Gaynutdinova Elza Mukhanina Ekaterina Shayakhmetova Lilia Bagavieva Elmira Fattakhova Zilya Akhmetov Tahir Zagidullin Lenar Haertdinov Ravil |
| author_facet | Safina Natalia Shakirov Shamil Gaynutdinova Elza Mukhanina Ekaterina Shayakhmetova Lilia Bagavieva Elmira Fattakhova Zilya Akhmetov Tahir Zagidullin Lenar Haertdinov Ravil |
| author_sort | Safina Natalia |
| collection | DOAJ |
| description | The study examined the polymorphism of the glutathione peroxidase-1 gene (GPX-1, g. 189 T/C) effect on the GPx enzyme activity and biochemical parameters in the blood serum of Holstein cows of indigenous and foreign selection. The work was carried out in the Lenin Breeding Farm Integrated Agricultural Production Center (IAPC) and the Mukhametshin Z.Z. Peasant Farm Enterprise (PFE) of the Republic of Tatarstan. DNA genotyping by the locus of the Bos taurus GPX-1-Bsc4 I gene was performed by PCR-RFLP. As a result of genetic typing of cattle, 2 alleles (C and T) and 3 genotypes (CC, TC and TT) were identified. The classification carried out by PIC values in two populations of Holstein cattle showed that there is a moderate polymorphism of the GPX-1 gene. A negative inbreeding coefficient (F) indicates excessive outbreeding in both populations. In both populations, the numerical advantage of heterozygous genotype TC cows is 60.1 and 56.7 % of the total livestock. Indigenous breeding cows, compared with imported cattle had higher: GPx activity, AST, ALT, cholesterol level, total protein and albumin fraction. Analysis of the association between the GPX-1 gene polymorphism and the GPx enzyme level indicates that the activity of this enzyme varies depending on the genotype, and there is also a variation in the biochemical parameters of the Holstein cattle blood serum. |
| first_indexed | 2024-03-08T11:12:36Z |
| format | Article |
| id | doaj.art-9edea3191b814c648c4c85d1e91024b3 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-08T11:12:36Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-9edea3191b814c648c4c85d1e91024b32024-01-26T10:40:48ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014620101810.1051/e3sconf/202346201018e3sconf_afe23_01018Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattleSafina Natalia0Shakirov Shamil1Gaynutdinova Elza2Mukhanina Ekaterina3Shayakhmetova Lilia4Bagavieva Elmira5Fattakhova Zilya6Akhmetov Tahir7Zagidullin Lenar8Haertdinov Ravil9Tatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of SciencesTatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of SciencesTatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of SciencesTatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of SciencesTatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of SciencesTatar Scientific Research Institute of Agriculture of FRC Kazan Scientific Center of Russia Academy of SciencesN.E. Bauman Kazan State Academy of Veterinary Medicine Federal State Budgetary Institution of Higher EducationN.E. Bauman Kazan State Academy of Veterinary Medicine Federal State Budgetary Institution of Higher EducationN.E. Bauman Kazan State Academy of Veterinary Medicine Federal State Budgetary Institution of Higher EducationN.E. Bauman Kazan State Academy of Veterinary Medicine Federal State Budgetary Institution of Higher EducationThe study examined the polymorphism of the glutathione peroxidase-1 gene (GPX-1, g. 189 T/C) effect on the GPx enzyme activity and biochemical parameters in the blood serum of Holstein cows of indigenous and foreign selection. The work was carried out in the Lenin Breeding Farm Integrated Agricultural Production Center (IAPC) and the Mukhametshin Z.Z. Peasant Farm Enterprise (PFE) of the Republic of Tatarstan. DNA genotyping by the locus of the Bos taurus GPX-1-Bsc4 I gene was performed by PCR-RFLP. As a result of genetic typing of cattle, 2 alleles (C and T) and 3 genotypes (CC, TC and TT) were identified. The classification carried out by PIC values in two populations of Holstein cattle showed that there is a moderate polymorphism of the GPX-1 gene. A negative inbreeding coefficient (F) indicates excessive outbreeding in both populations. In both populations, the numerical advantage of heterozygous genotype TC cows is 60.1 and 56.7 % of the total livestock. Indigenous breeding cows, compared with imported cattle had higher: GPx activity, AST, ALT, cholesterol level, total protein and albumin fraction. Analysis of the association between the GPX-1 gene polymorphism and the GPx enzyme level indicates that the activity of this enzyme varies depending on the genotype, and there is also a variation in the biochemical parameters of the Holstein cattle blood serum.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/99/e3sconf_afe23_01018.pdf |
| spellingShingle | Safina Natalia Shakirov Shamil Gaynutdinova Elza Mukhanina Ekaterina Shayakhmetova Lilia Bagavieva Elmira Fattakhova Zilya Akhmetov Tahir Zagidullin Lenar Haertdinov Ravil Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle E3S Web of Conferences |
| title | Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle |
| title_full | Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle |
| title_fullStr | Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle |
| title_full_unstemmed | Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle |
| title_short | Polymorphism of the glutathioneperoxidase-1 gene (GPX-1 g. 189 T/C) and biochemical parameters of the blood serum of Holstein cattle |
| title_sort | polymorphism of the glutathioneperoxidase 1 gene gpx 1 g 189 t c and biochemical parameters of the blood serum of holstein cattle |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/99/e3sconf_afe23_01018.pdf |
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