Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal
The <i>Mycobacterium abscessus</i> complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15402 |
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| author | Sofia Carneiro Miguel Pinto Sónia Silva Andrea Santos Irene Rodrigues Daniela Santos Sílvia Duarte Luís Vieira João Paulo Gomes Rita Macedo |
| author_facet | Sofia Carneiro Miguel Pinto Sónia Silva Andrea Santos Irene Rodrigues Daniela Santos Sílvia Duarte Luís Vieira João Paulo Gomes Rita Macedo |
| author_sort | Sofia Carneiro |
| collection | DOAJ |
| description | The <i>Mycobacterium abscessus</i> complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have been genetically identified as groups of strains associated with higher prevalence, higher levels of antimicrobial resistance, and worse clinical outcomes. To date, little is known about the genomic characteristics of MABC species circulating in Portugal. Here, we examined the genetic diversity and antimicrobial resistance profiles of 30 MABC strains isolated between 2014 and 2022 in Portugal. The genetic diversity of circulating MABC strains was assessed through a gene-by-gene approach (wgMLST), allowing their subspecies differentiation and the classification of isolates into DCCs. Antimicrobial resistance profiles were defined using phenotypic, molecular, and genomic approaches. The majority of isolates were resistant to at least two antimicrobials, although a poor correlation between phenotype and genotype data was observed. Portuguese genomes were highly diverse, and data suggest the existence of MABC lineages with potential international circulation or cross-border transmission. This study highlights the genetic diversity and antimicrobial resistance profile of circulating MABC isolates in Portugal while representing the first step towards the implementation of a genomic-based surveillance system for MABC at the Portuguese NIH. |
| first_indexed | 2024-03-10T21:11:57Z |
| format | Article |
| id | doaj.art-9ee19a99875147e5ae4d1efdb1f95df4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:11:57Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9ee19a99875147e5ae4d1efdb1f95df42023-11-19T16:46:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201540210.3390/ijms242015402Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from PortugalSofia Carneiro0Miguel Pinto1Sónia Silva2Andrea Santos3Irene Rodrigues4Daniela Santos5Sílvia Duarte6Luís Vieira7João Paulo Gomes8Rita Macedo9National Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalGenomics and Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalNational Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalNational Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalNational Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalTechnology and Innovation Unit, Department of Human Genetics, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalTechnology and Innovation Unit, Department of Human Genetics, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalTechnology and Innovation Unit, Department of Human Genetics, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalGenomics and Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalNational Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, PortugalThe <i>Mycobacterium abscessus</i> complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have been genetically identified as groups of strains associated with higher prevalence, higher levels of antimicrobial resistance, and worse clinical outcomes. To date, little is known about the genomic characteristics of MABC species circulating in Portugal. Here, we examined the genetic diversity and antimicrobial resistance profiles of 30 MABC strains isolated between 2014 and 2022 in Portugal. The genetic diversity of circulating MABC strains was assessed through a gene-by-gene approach (wgMLST), allowing their subspecies differentiation and the classification of isolates into DCCs. Antimicrobial resistance profiles were defined using phenotypic, molecular, and genomic approaches. The majority of isolates were resistant to at least two antimicrobials, although a poor correlation between phenotype and genotype data was observed. Portuguese genomes were highly diverse, and data suggest the existence of MABC lineages with potential international circulation or cross-border transmission. This study highlights the genetic diversity and antimicrobial resistance profile of circulating MABC isolates in Portugal while representing the first step towards the implementation of a genomic-based surveillance system for MABC at the Portuguese NIH.https://www.mdpi.com/1422-0067/24/20/15402<i>Mycobacterium abscessus</i>MABCnontuberculous mycobacteriawhole-genome sequencingwgMLSTantimicrobial resistance |
| spellingShingle | Sofia Carneiro Miguel Pinto Sónia Silva Andrea Santos Irene Rodrigues Daniela Santos Sílvia Duarte Luís Vieira João Paulo Gomes Rita Macedo Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal International Journal of Molecular Sciences <i>Mycobacterium abscessus</i> MABC nontuberculous mycobacteria whole-genome sequencing wgMLST antimicrobial resistance |
| title | Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal |
| title_full | Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal |
| title_fullStr | Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal |
| title_full_unstemmed | Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal |
| title_short | Genome-Scale Characterization of <i>Mycobacterium abscessus</i> Complex Isolates from Portugal |
| title_sort | genome scale characterization of i mycobacterium abscessus i complex isolates from portugal |
| topic | <i>Mycobacterium abscessus</i> MABC nontuberculous mycobacteria whole-genome sequencing wgMLST antimicrobial resistance |
| url | https://www.mdpi.com/1422-0067/24/20/15402 |
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