Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold
The main aim of this paper is to achieve the suitable SA-GEL (sodium alginate and gelatin) porous cartilage scaffold by 3D printing technology with optimal prediction parameters. Firstly, the characteristics of SA-GEL were analyzed, the influence of calcium chloride on the gel was explored, and the...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/3/768 |
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| author | Youping Gong Fei Wang M. S. H. Al-Furjan Lijun Shan Jingyang He Xiangjuan Bian Zhikai Bi Haiqiang Liu Wenxin Li Huifeng Shao Guojin Chen Abu Bakar Sulong |
| author_facet | Youping Gong Fei Wang M. S. H. Al-Furjan Lijun Shan Jingyang He Xiangjuan Bian Zhikai Bi Haiqiang Liu Wenxin Li Huifeng Shao Guojin Chen Abu Bakar Sulong |
| author_sort | Youping Gong |
| collection | DOAJ |
| description | The main aim of this paper is to achieve the suitable SA-GEL (sodium alginate and gelatin) porous cartilage scaffold by 3D printing technology with optimal prediction parameters. Firstly, the characteristics of SA-GEL were analyzed, the influence of calcium chloride on the gel was explored, and the optimal cross-linking concentration and gelation temperature were determined. Secondly, a prediction model of the extrusion line width of SA-GEL was established, in which the printing pressure, the moving speed of the needle and the fiber interval were the important parameters affecting the printing performance of the SA-GEL composite material. Thirdly, the SA-GEL composite scaffolds were printed on the Bio-plotter platform, the C5.18 chondrocytes cells were cultured in the SA-GEL biomaterial scaffold, and the results show that the cells could survive well. These results show that, under the control of the printing parameters pressure 1.8 bar, moving speed 10.7 mm/s and the internal structure parameters of the scaffold is 0/45-1.2 (Printing interval: 1.2 mm, angle value: 45 degree), SA-GEL scaffold printing results can be obtained which have good mechanical properties and biocompatibility. |
| first_indexed | 2024-12-19T09:26:16Z |
| format | Article |
| id | doaj.art-9ee661ffb3734a48a5b4dcaefb4672e4 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-19T09:26:16Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9ee661ffb3734a48a5b4dcaefb4672e42022-12-21T20:27:48ZengMDPI AGApplied Sciences2076-34172020-01-0110376810.3390/app10030768app10030768Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage ScaffoldYouping Gong0Fei Wang1M. S. H. Al-Furjan2Lijun Shan3Jingyang He4Xiangjuan Bian5Zhikai Bi6Haiqiang Liu7Wenxin Li8Huifeng Shao9Guojin Chen10Abu Bakar Sulong11School of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaDepartment of Chemical & Process Engineering, Faculty of Engineering & Built Environment, University Kebangsaan Malaysia, Bangi 43600, MalaysiaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaFaculty of Science and Technology, Zhejiang International Studies University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaSchool of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, ChinaDepartment of Mechanical and Materials Engineering, Faculty of Engineering & Built Environment, University Kebangsaan Malaysia, Bangi 43600, MalaysiaThe main aim of this paper is to achieve the suitable SA-GEL (sodium alginate and gelatin) porous cartilage scaffold by 3D printing technology with optimal prediction parameters. Firstly, the characteristics of SA-GEL were analyzed, the influence of calcium chloride on the gel was explored, and the optimal cross-linking concentration and gelation temperature were determined. Secondly, a prediction model of the extrusion line width of SA-GEL was established, in which the printing pressure, the moving speed of the needle and the fiber interval were the important parameters affecting the printing performance of the SA-GEL composite material. Thirdly, the SA-GEL composite scaffolds were printed on the Bio-plotter platform, the C5.18 chondrocytes cells were cultured in the SA-GEL biomaterial scaffold, and the results show that the cells could survive well. These results show that, under the control of the printing parameters pressure 1.8 bar, moving speed 10.7 mm/s and the internal structure parameters of the scaffold is 0/45-1.2 (Printing interval: 1.2 mm, angle value: 45 degree), SA-GEL scaffold printing results can be obtained which have good mechanical properties and biocompatibility.https://www.mdpi.com/2076-3417/10/3/768biological 3d printingsodium alginategelatincartilage scaffold |
| spellingShingle | Youping Gong Fei Wang M. S. H. Al-Furjan Lijun Shan Jingyang He Xiangjuan Bian Zhikai Bi Haiqiang Liu Wenxin Li Huifeng Shao Guojin Chen Abu Bakar Sulong Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold Applied Sciences biological 3d printing sodium alginate gelatin cartilage scaffold |
| title | Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold |
| title_full | Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold |
| title_fullStr | Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold |
| title_full_unstemmed | Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold |
| title_short | Experimental Investigation and Optimal 3D Bioprinting Parameters of SA-Gel Porous Cartilage Scaffold |
| title_sort | experimental investigation and optimal 3d bioprinting parameters of sa gel porous cartilage scaffold |
| topic | biological 3d printing sodium alginate gelatin cartilage scaffold |
| url | https://www.mdpi.com/2076-3417/10/3/768 |
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