Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts
This study investigated the beneficial properties of prickly pear peel (PPP) extracts from <i>Opuntia ficus-indica</i> (L.) Mill. Extracts were obtained via the Soxhlet extraction method using methanol (P1), ethanol (P2) and ethanol-water (P3) as extraction solvents. Their total phenolic...
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/7/1537 |
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| author | Margarita Dormousoglou Ioanna Efthimiou Maria Antonopoulou Stefanos Dailianis Giulia Herbst Dimitris Vlastos |
| author_facet | Margarita Dormousoglou Ioanna Efthimiou Maria Antonopoulou Stefanos Dailianis Giulia Herbst Dimitris Vlastos |
| author_sort | Margarita Dormousoglou |
| collection | DOAJ |
| description | This study investigated the beneficial properties of prickly pear peel (PPP) extracts from <i>Opuntia ficus-indica</i> (L.) Mill. Extracts were obtained via the Soxhlet extraction method using methanol (P1), ethanol (P2) and ethanol-water (P3) as extraction solvents. Their total phenolic and flavonoid content (TPC and TFC, respectively) and their antioxidant activity (AA) were determined. The PPP extracts were characterized in detail using mass spectrometry techniques. Their cyto-genotoxic effect and antigenotoxic potential against mitomycin C were evaluated via the cytokinesis block micronucleus (CBMN) assay on human lymphocytes. Enhanced TPC, TFC and AA values were recorded for all the extracts. Moreover, P1 and P2 were cytotoxic only at the highest concentrations, whereas P3 was found to be cytotoxic in all cases. No significant micronucleus induction was observed in the tested extracts. The PPP extracts contain bioactive compounds such as flavonoids, carboxylic acids, alkaloids, fatty acids and minerals (mainly K, Si, Mg, Ca, P and Zn). The results showed that all three extracts exerted high antigenotoxic activity. Our findings confirm the beneficial and genoprotective properties of PPP extracts and further studies on the bioactive compounds of <i>Opuntia ficus-indica</i> (L.) Mill. are recommended, as it constitutes a promising plant in pharmaceutical applications. |
| first_indexed | 2024-03-11T05:27:59Z |
| format | Article |
| id | doaj.art-9ee677e14680402d82f026636fa18e91 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T05:27:59Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9ee677e14680402d82f026636fa18e912023-11-17T17:24:00ZengMDPI AGPlants2223-77472023-04-01127153710.3390/plants12071537Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel ExtractsMargarita Dormousoglou0Ioanna Efthimiou1Maria Antonopoulou2Stefanos Dailianis3Giulia Herbst4Dimitris Vlastos5Department of Sustainable Agriculture (Former Department of Environmental Engineering), University of Patras, Seferi 2, GR-30100 Agrinio, GreeceDepartment of Sustainable Agriculture (Former Department of Environmental Engineering), University of Patras, Seferi 2, GR-30100 Agrinio, GreeceDepartment of Sustainable Agriculture (Former Department of Environmental Engineering), University of Patras, Seferi 2, GR-30100 Agrinio, GreeceDepartment of Biology, University of Patras, GR-26500 Patras, GreeceDepartment of Chemical Engineering, Federal University of Paraná, Curitiba 81531-990, PR, BrazilDepartment of Biology, University of Patras, GR-26500 Patras, GreeceThis study investigated the beneficial properties of prickly pear peel (PPP) extracts from <i>Opuntia ficus-indica</i> (L.) Mill. Extracts were obtained via the Soxhlet extraction method using methanol (P1), ethanol (P2) and ethanol-water (P3) as extraction solvents. Their total phenolic and flavonoid content (TPC and TFC, respectively) and their antioxidant activity (AA) were determined. The PPP extracts were characterized in detail using mass spectrometry techniques. Their cyto-genotoxic effect and antigenotoxic potential against mitomycin C were evaluated via the cytokinesis block micronucleus (CBMN) assay on human lymphocytes. Enhanced TPC, TFC and AA values were recorded for all the extracts. Moreover, P1 and P2 were cytotoxic only at the highest concentrations, whereas P3 was found to be cytotoxic in all cases. No significant micronucleus induction was observed in the tested extracts. The PPP extracts contain bioactive compounds such as flavonoids, carboxylic acids, alkaloids, fatty acids and minerals (mainly K, Si, Mg, Ca, P and Zn). The results showed that all three extracts exerted high antigenotoxic activity. Our findings confirm the beneficial and genoprotective properties of PPP extracts and further studies on the bioactive compounds of <i>Opuntia ficus-indica</i> (L.) Mill. are recommended, as it constitutes a promising plant in pharmaceutical applications.https://www.mdpi.com/2223-7747/12/7/1537prickly pear peel<i>Opuntia ficus-indica</i>plant extractsCBMN assaygenotoxicityantigenotoxicity |
| spellingShingle | Margarita Dormousoglou Ioanna Efthimiou Maria Antonopoulou Stefanos Dailianis Giulia Herbst Dimitris Vlastos Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts Plants prickly pear peel <i>Opuntia ficus-indica</i> plant extracts CBMN assay genotoxicity antigenotoxicity |
| title | Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts |
| title_full | Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts |
| title_fullStr | Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts |
| title_full_unstemmed | Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts |
| title_short | Phytochemical Analysis and Genotoxicological Evaluation of Prickly Pear Peel Extracts |
| title_sort | phytochemical analysis and genotoxicological evaluation of prickly pear peel extracts |
| topic | prickly pear peel <i>Opuntia ficus-indica</i> plant extracts CBMN assay genotoxicity antigenotoxicity |
| url | https://www.mdpi.com/2223-7747/12/7/1537 |
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