Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure

<i>Background and Objectives:</i> Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prost...

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Main Authors: Darijus Skaudickas, Gintautas Vaitiekaitis, Julius Liobikas, Aldona Gružienė, Marcel Abras, Gita Gersone, Aleksandras Vitkus, Sigita Kerzienė, Greta Undžytė, Vincentas Veikutis, Artūras Kašauskas, Armuntas Baginskas, Algis Noreika
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Medicina
Subjects:
Online Access:https://www.mdpi.com/1648-9144/59/4/702
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author Darijus Skaudickas
Gintautas Vaitiekaitis
Julius Liobikas
Aldona Gružienė
Marcel Abras
Gita Gersone
Aleksandras Vitkus
Sigita Kerzienė
Greta Undžytė
Vincentas Veikutis
Artūras Kašauskas
Armuntas Baginskas
Algis Noreika
author_facet Darijus Skaudickas
Gintautas Vaitiekaitis
Julius Liobikas
Aldona Gružienė
Marcel Abras
Gita Gersone
Aleksandras Vitkus
Sigita Kerzienė
Greta Undžytė
Vincentas Veikutis
Artūras Kašauskas
Armuntas Baginskas
Algis Noreika
author_sort Darijus Skaudickas
collection DOAJ
description <i>Background and Objectives:</i> Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prostate tissues. <i>Materials and Methods:</i> A standard prostate RFA procedure was performed on 13 non-purebred dogs in three sessions: no cooling (NC), cooling with a 0.1% NaCl solution (C.01), and cooling using a 0.9% NaCl solution (C.09). Microtome-cut 2–3 µm sections of prostate samples were stained with hematoxylin and eosin and further examined. <i>Results:</i> A histopathologic evaluation identified four zones of exposure: direct, application, necrosis, and transitional, as the damage on tissues decreased going further from the ablation site. The areas and perimeters of these zones were calculated, and geometric shapes of ablative lesions were evaluated using the quotient formula. Areas and perimeters of prostate tissue lesions in the NC and C.09 sessions were of similar size, whereas those found in C.01 were statistically significantly smaller. Lesions observed in session C.01 were of the most regular geometric shape, while the most irregular ones were found in session C.09. The shapes of lesions closest to the ablation electrode were the most irregular, becoming more regular the further away from the electrode they were. <i>Conclusions:</i> Prostate RFA leads to tissue damage with distinct morphological zones. Notably, the prostate lesions were the smallest and the most regular in shape after RFA procedures using the 0.1% NaCl cooling solution. It can be argued that smaller ablation sites may result in smaller scars, thus allowing for faster tissue healing if the blood flow and innervation at the ablation site are not compromised.
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spelling doaj.art-9ee84ff021134e8cb224ecef486100f72023-11-17T20:20:46ZengMDPI AGMedicina1010-660X1648-91442023-04-0159470210.3390/medicina59040702Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation ProcedureDarijus Skaudickas0Gintautas Vaitiekaitis1Julius Liobikas2Aldona Gružienė3Marcel Abras4Gita Gersone5Aleksandras Vitkus6Sigita Kerzienė7Greta Undžytė8Vincentas Veikutis9Artūras Kašauskas10Armuntas Baginskas11Algis Noreika12Medical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaDepartment of Internal Medicine, Nicolae Testemitanu State University of Medicine and Pharmacy, MD2004 Chisinau, MoldovaDepartment of Human Physiology and Biochemistry, Rīga Stradiņš University, LV-1007 Riga, LatviaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaMedical Academy, Lithuanian University of Health Sciences, LT50161 Kaunas, LithuaniaVeterinary Academy, Lithuanian University of Health Sciences, LT47181 Kaunas, Lithuania<i>Background and Objectives:</i> Prostate cancer is on the rise in the European Union, and radiofrequency ablation (RFA) is one of the minimally invasive treatment options used for its treatment. Therefore, the aim of this study was to investigate and analyze the effects of RFA on prostate tissues. <i>Materials and Methods:</i> A standard prostate RFA procedure was performed on 13 non-purebred dogs in three sessions: no cooling (NC), cooling with a 0.1% NaCl solution (C.01), and cooling using a 0.9% NaCl solution (C.09). Microtome-cut 2–3 µm sections of prostate samples were stained with hematoxylin and eosin and further examined. <i>Results:</i> A histopathologic evaluation identified four zones of exposure: direct, application, necrosis, and transitional, as the damage on tissues decreased going further from the ablation site. The areas and perimeters of these zones were calculated, and geometric shapes of ablative lesions were evaluated using the quotient formula. Areas and perimeters of prostate tissue lesions in the NC and C.09 sessions were of similar size, whereas those found in C.01 were statistically significantly smaller. Lesions observed in session C.01 were of the most regular geometric shape, while the most irregular ones were found in session C.09. The shapes of lesions closest to the ablation electrode were the most irregular, becoming more regular the further away from the electrode they were. <i>Conclusions:</i> Prostate RFA leads to tissue damage with distinct morphological zones. Notably, the prostate lesions were the smallest and the most regular in shape after RFA procedures using the 0.1% NaCl cooling solution. It can be argued that smaller ablation sites may result in smaller scars, thus allowing for faster tissue healing if the blood flow and innervation at the ablation site are not compromised.https://www.mdpi.com/1648-9144/59/4/702radiofrequency ablationprostatehistology of prostate zonesmorphologyelectrode
spellingShingle Darijus Skaudickas
Gintautas Vaitiekaitis
Julius Liobikas
Aldona Gružienė
Marcel Abras
Gita Gersone
Aleksandras Vitkus
Sigita Kerzienė
Greta Undžytė
Vincentas Veikutis
Artūras Kašauskas
Armuntas Baginskas
Algis Noreika
Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
Medicina
radiofrequency ablation
prostate
histology of prostate zones
morphology
electrode
title Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_full Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_fullStr Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_full_unstemmed Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_short Analysis of Morphological and Morphometric Changes in a Parenchymal Tissue after the Radiofrequency Ablation Procedure
title_sort analysis of morphological and morphometric changes in a parenchymal tissue after the radiofrequency ablation procedure
topic radiofrequency ablation
prostate
histology of prostate zones
morphology
electrode
url https://www.mdpi.com/1648-9144/59/4/702
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