Genetic identification for Staphylococcus aureus by using nuc gene
To examine the recovered strains phenotypically by conventional methods and genotypically by using synthetic oligonucleotides of 21 and 24 bases in polymerase chain reaction to amplify a sequence of nuc gene , which encoded thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Anbar
2015-12-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_127590_35bd7a0cb21cf6c6d487d6deca1a2944.pdf |
| _version_ | 1797373216637845504 |
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| author | Mushtaq T. Salih Ahmed M. Turkey Mayada A. Shihan |
| author_facet | Mushtaq T. Salih Ahmed M. Turkey Mayada A. Shihan |
| author_sort | Mushtaq T. Salih |
| collection | DOAJ |
| description | To examine the recovered strains phenotypically by conventional methods and genotypically by using synthetic oligonucleotides of 21 and 24 bases in polymerase chain reaction to amplify a sequence of nuc gene , which encoded thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from isolated DNA, PCR products was detected by agarose gel electrophoresis. Our results showed that 100% of Staphylococcus aureus were positive to nuc gene .The PCR for amplification of nuc gene has potential for rapid diagnosis of S. aureus infections by direct testing of clinical specimens . |
| first_indexed | 2024-03-08T18:47:10Z |
| format | Article |
| id | doaj.art-9ee89070ebed438ea79ea7882f2a6ecc |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:47:10Z |
| publishDate | 2015-12-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-9ee89070ebed438ea79ea7882f2a6ecc2023-12-28T21:58:13ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032015-12-0193667110.37652/juaps.2015.127590127590Genetic identification for Staphylococcus aureus by using nuc geneMushtaq T. Salih0Ahmed M. Turkey1Mayada A. Shihan2University of Anbar - College of PharmacyUniversity of Anbar - College of ScienceUniversity of Anbar - College of ScienceTo examine the recovered strains phenotypically by conventional methods and genotypically by using synthetic oligonucleotides of 21 and 24 bases in polymerase chain reaction to amplify a sequence of nuc gene , which encoded thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from isolated DNA, PCR products was detected by agarose gel electrophoresis. Our results showed that 100% of Staphylococcus aureus were positive to nuc gene .The PCR for amplification of nuc gene has potential for rapid diagnosis of S. aureus infections by direct testing of clinical specimens .https://juaps.uoanbar.edu.iq/article_127590_35bd7a0cb21cf6c6d487d6deca1a2944.pdfnuc gene |
| spellingShingle | Mushtaq T. Salih Ahmed M. Turkey Mayada A. Shihan Genetic identification for Staphylococcus aureus by using nuc gene مجلة جامعة الانبار للعلوم الصرفة nuc gene |
| title | Genetic identification for Staphylococcus aureus by using nuc gene |
| title_full | Genetic identification for Staphylococcus aureus by using nuc gene |
| title_fullStr | Genetic identification for Staphylococcus aureus by using nuc gene |
| title_full_unstemmed | Genetic identification for Staphylococcus aureus by using nuc gene |
| title_short | Genetic identification for Staphylococcus aureus by using nuc gene |
| title_sort | genetic identification for staphylococcus aureus by using nuc gene |
| topic | nuc gene |
| url | https://juaps.uoanbar.edu.iq/article_127590_35bd7a0cb21cf6c6d487d6deca1a2944.pdf |
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