Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats
Background and purpose: Pulmonary fibrosis is a chronic disease of the lungs caused by inflammation, species of reactive oxygen, and immune defects. Antioxidant properties of Nasturtium officinale has been reported in some studies. Therefore, the objective of the current study was to evaluate the ef...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Research in Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2021;volume=16;issue=1;spage=94;epage=102;aulast=Ramezani |
| _version_ | 1818355076805165056 |
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| author | Sanaz Ramezani Iraj Javadi Esmaeel Panahi Kokhdan Navid Omidifar Jafar Nikbakht Heibatollah Sadeghi Amir Hossein Doustimotlagh Nazanin Danaei Reza Abbasi Hossein Sadeghi |
| author_facet | Sanaz Ramezani Iraj Javadi Esmaeel Panahi Kokhdan Navid Omidifar Jafar Nikbakht Heibatollah Sadeghi Amir Hossein Doustimotlagh Nazanin Danaei Reza Abbasi Hossein Sadeghi |
| author_sort | Sanaz Ramezani |
| collection | DOAJ |
| description | Background and purpose: Pulmonary fibrosis is a chronic disease of the lungs caused by inflammation, species of reactive oxygen, and immune defects. Antioxidant properties of Nasturtium officinale has been reported in some studies. Therefore, the objective of the current study was to evaluate the effect of ethanolic extract of Nasturtium officinale (EENO) on bleomycin (BLM)-induced lung fibrosis in rats.
Experimental approach: Forty adult male Wistar rats (180-220 g) were randomly divided into 5 experimental groups. Normal control, BLM control received a single dose of BLM (6 IU/kg) intratracheally only on the first day, EENO + BLM group received EENO (500 mg/kg) one week before intratracheal BLM instillation and two weeks afterward, BLM + EENO group and BML + vitamin E group received EENO (500 mg/kg) and vitamin E (500 mg/kg) half-hour after BLM installation, respectively. The animals were sacrificed on day 22. Change in body weight, lung index, serum level of malondialdehyde (MDA) and nitric oxide (NO) metabolite, lung tissue hydroxyproline content and lung pathology were assessed.
Findings/Results: Pre- or post-treatment with EENO attenuated pulmonary fibrosis as evidenced by normalized lung index, improved histological changes and inhibited collagen deposition (hydroxyproline) in the animal lung. EENO also decreased MDA and NO metabolite release in comparison to the BLM control. vitamin E (500 mg/ kg) also significantly inhibited the BLM-induced lung toxicity.
Conclusions and implications: EENO can prevent BLM-induced lung fibrosis in rats via antioxidant activities. However, more studies are needed to elicit the exact mechanism of this effect. |
| first_indexed | 2024-12-13T19:35:34Z |
| format | Article |
| id | doaj.art-9eeae6b7d22c4bfaaec13d6f2cc6d919 |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-13T19:35:34Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-9eeae6b7d22c4bfaaec13d6f2cc6d9192022-12-21T23:33:50ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142021-01-011619410210.4103/1735-5362.305192Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in ratsSanaz RamezaniIraj JavadiEsmaeel Panahi KokhdanNavid OmidifarJafar NikbakhtHeibatollah SadeghiAmir Hossein DoustimotlaghNazanin DanaeiReza AbbasiHossein SadeghiBackground and purpose: Pulmonary fibrosis is a chronic disease of the lungs caused by inflammation, species of reactive oxygen, and immune defects. Antioxidant properties of Nasturtium officinale has been reported in some studies. Therefore, the objective of the current study was to evaluate the effect of ethanolic extract of Nasturtium officinale (EENO) on bleomycin (BLM)-induced lung fibrosis in rats. Experimental approach: Forty adult male Wistar rats (180-220 g) were randomly divided into 5 experimental groups. Normal control, BLM control received a single dose of BLM (6 IU/kg) intratracheally only on the first day, EENO + BLM group received EENO (500 mg/kg) one week before intratracheal BLM instillation and two weeks afterward, BLM + EENO group and BML + vitamin E group received EENO (500 mg/kg) and vitamin E (500 mg/kg) half-hour after BLM installation, respectively. The animals were sacrificed on day 22. Change in body weight, lung index, serum level of malondialdehyde (MDA) and nitric oxide (NO) metabolite, lung tissue hydroxyproline content and lung pathology were assessed. Findings/Results: Pre- or post-treatment with EENO attenuated pulmonary fibrosis as evidenced by normalized lung index, improved histological changes and inhibited collagen deposition (hydroxyproline) in the animal lung. EENO also decreased MDA and NO metabolite release in comparison to the BLM control. vitamin E (500 mg/ kg) also significantly inhibited the BLM-induced lung toxicity. Conclusions and implications: EENO can prevent BLM-induced lung fibrosis in rats via antioxidant activities. However, more studies are needed to elicit the exact mechanism of this effect.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2021;volume=16;issue=1;spage=94;epage=102;aulast=Ramezanibleomycin; fibrosis; nasturtium officinale; vitamin e |
| spellingShingle | Sanaz Ramezani Iraj Javadi Esmaeel Panahi Kokhdan Navid Omidifar Jafar Nikbakht Heibatollah Sadeghi Amir Hossein Doustimotlagh Nazanin Danaei Reza Abbasi Hossein Sadeghi Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats Research in Pharmaceutical Sciences bleomycin; fibrosis; nasturtium officinale; vitamin e |
| title | Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats |
| title_full | Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats |
| title_fullStr | Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats |
| title_full_unstemmed | Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats |
| title_short | Protective and therapeutic effects of ethanolic extract of Nasturtium officinale (watercress) and vitamin E against bleomycin-induced pulmonary fibrosis in rats |
| title_sort | protective and therapeutic effects of ethanolic extract of nasturtium officinale watercress and vitamin e against bleomycin induced pulmonary fibrosis in rats |
| topic | bleomycin; fibrosis; nasturtium officinale; vitamin e |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2021;volume=16;issue=1;spage=94;epage=102;aulast=Ramezani |
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