Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites
BackgroundDietary polyphenols, polypeptides, and oligosaccharides modulate inflammation and immunity by altering the composition of gut microbiota. The polyphenols and polypeptides in Chinese rice wine have protective effects against cardiovascular disease. In this study, we hypothesized that the po...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.940663/full |
| _version_ | 1828231073737211904 |
|---|---|
| author | Jinjin Yang Jiaoying Song Jiedong Zhou Hui Lin Zhuonan Wu Nan Liu Wenqing Xie Hangyuan Guo Jufang Chi |
| author_facet | Jinjin Yang Jiaoying Song Jiedong Zhou Hui Lin Zhuonan Wu Nan Liu Wenqing Xie Hangyuan Guo Jufang Chi |
| author_sort | Jinjin Yang |
| collection | DOAJ |
| description | BackgroundDietary polyphenols, polypeptides, and oligosaccharides modulate inflammation and immunity by altering the composition of gut microbiota. The polyphenols and polypeptides in Chinese rice wine have protective effects against cardiovascular disease. In this study, we hypothesized that the polyphenols, polypeptides, and oligosaccharides in Chinese rice wine can ameliorate diabetic cardiomyopathy (DCM) by altering gut microbiota and metabolites.MethodsMice with DCM and high glucose cells were treated with rice wine polyphenols (RWPH), rice wine polypeptides (RWPE), and rice wine oligosaccharides. Cardiac function was evaluated by echocardiography and detection of myocardial injury markers. We observed the pathological structures using hematoxylin and eosin staining, Masson's trichrome staining, and transmission electron microscopy. The expression levels of autophagy-related proteins and stubRFP-sensGFP-LC3 fluorescence were measured to evaluate autophagy. We performed TUNEL staining and measured the levels of Bax, Bcl-2, and p53 to assess apoptosis. To analyze the effects of the rice wine functional components on the gut microbiota and metabolites of DCM mice, we performed fecal 16S-rDNA gene sequencing and serum untargeted metabolomics.ResultsOur results showed an increase in cardiac and mitochondrial function, promotion of autophagy, and inhibition of cardiomyocyte apoptosis, which indicates that RWPH and RWPE can ameliorate DCM. The abundance of Akkermansia and Desulfovibrio were reduced by the presence of RWPH and RWPE. The growth of the Lachnospiraceae_NK4A136_group and Clostridiales-unclassified were promoted by the presence of RWPH. Tryptophan metabolism-associated metabolites were increased and phenylalanine levels were reduced by the presence of RWPH and RWPE. The biosynthesis of primary bile acids was enhanced by the presence of RWPH.ConclusionBoth RWPH and RWPE provided a protective effect against DCM by promoting autophagy, inhibiting apoptosis, and reversing both gut microbiota dysbiosis and metabolic dysregulation. |
| first_indexed | 2024-04-12T18:57:57Z |
| format | Article |
| id | doaj.art-9eebbda8a62d4091a6ff4c7ccf206eb8 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-12T18:57:57Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-9eebbda8a62d4091a6ff4c7ccf206eb82022-12-22T03:20:15ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-09-01910.3389/fcvm.2022.940663940663Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolitesJinjin Yang0Jiaoying Song1Jiedong Zhou2Hui Lin3Zhuonan Wu4Nan Liu5Wenqing Xie6Hangyuan Guo7Jufang Chi8Department of Cardiology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, ChinaMedical College of Shaoxing University, Shaoxing, ChinaMedical College of Shaoxing University, Shaoxing, ChinaDepartment of Cardiology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, ChinaMedical College of Shaoxing University, Shaoxing, ChinaSecond Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaSecond Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, ChinaMedical College of Shaoxing University, Shaoxing, ChinaDepartment of Cardiology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, ChinaBackgroundDietary polyphenols, polypeptides, and oligosaccharides modulate inflammation and immunity by altering the composition of gut microbiota. The polyphenols and polypeptides in Chinese rice wine have protective effects against cardiovascular disease. In this study, we hypothesized that the polyphenols, polypeptides, and oligosaccharides in Chinese rice wine can ameliorate diabetic cardiomyopathy (DCM) by altering gut microbiota and metabolites.MethodsMice with DCM and high glucose cells were treated with rice wine polyphenols (RWPH), rice wine polypeptides (RWPE), and rice wine oligosaccharides. Cardiac function was evaluated by echocardiography and detection of myocardial injury markers. We observed the pathological structures using hematoxylin and eosin staining, Masson's trichrome staining, and transmission electron microscopy. The expression levels of autophagy-related proteins and stubRFP-sensGFP-LC3 fluorescence were measured to evaluate autophagy. We performed TUNEL staining and measured the levels of Bax, Bcl-2, and p53 to assess apoptosis. To analyze the effects of the rice wine functional components on the gut microbiota and metabolites of DCM mice, we performed fecal 16S-rDNA gene sequencing and serum untargeted metabolomics.ResultsOur results showed an increase in cardiac and mitochondrial function, promotion of autophagy, and inhibition of cardiomyocyte apoptosis, which indicates that RWPH and RWPE can ameliorate DCM. The abundance of Akkermansia and Desulfovibrio were reduced by the presence of RWPH and RWPE. The growth of the Lachnospiraceae_NK4A136_group and Clostridiales-unclassified were promoted by the presence of RWPH. Tryptophan metabolism-associated metabolites were increased and phenylalanine levels were reduced by the presence of RWPH and RWPE. The biosynthesis of primary bile acids was enhanced by the presence of RWPH.ConclusionBoth RWPH and RWPE provided a protective effect against DCM by promoting autophagy, inhibiting apoptosis, and reversing both gut microbiota dysbiosis and metabolic dysregulation.https://www.frontiersin.org/articles/10.3389/fcvm.2022.940663/fulldiabetic cardiomyopathyautophagyapoptosisgut microbiomesmetabolomics |
| spellingShingle | Jinjin Yang Jiaoying Song Jiedong Zhou Hui Lin Zhuonan Wu Nan Liu Wenqing Xie Hangyuan Guo Jufang Chi Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites Frontiers in Cardiovascular Medicine diabetic cardiomyopathy autophagy apoptosis gut microbiomes metabolomics |
| title | Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites |
| title_full | Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites |
| title_fullStr | Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites |
| title_full_unstemmed | Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites |
| title_short | Functional components of Chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy, apoptosis, gut microbiota, and metabolites |
| title_sort | functional components of chinese rice wine can ameliorate diabetic cardiomyopathy through the modulation of autophagy apoptosis gut microbiota and metabolites |
| topic | diabetic cardiomyopathy autophagy apoptosis gut microbiomes metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.940663/full |
| work_keys_str_mv | AT jinjinyang functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT jiaoyingsong functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT jiedongzhou functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT huilin functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT zhuonanwu functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT nanliu functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT wenqingxie functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT hangyuanguo functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites AT jufangchi functionalcomponentsofchinesericewinecanamelioratediabeticcardiomyopathythroughthemodulationofautophagyapoptosisgutmicrobiotaandmetabolites |