Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil
Campylobacter jejuni is the most frequent cause of bacterial gastroenteritis; therefore, the characteristics of its epidemiology must be continuously investigated to support possible mitigating measures. This is particularly important when evaluating representative strains from the world's lead...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1220579/full |
| _version_ | 1797754533546295296 |
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| author | Phelipe Augusto Borba Martins Peres Roberta Torres de Melo Paulo Marcel Armendaris Fabiano Barreto Tiago Follmann Perin Ana Laura Grazziotin Guilherme Paz Monteiro Ana Beatriz Garcez Buiatte Eliane Pereira Mendonça Eduarda Cristina Alves Lourenzatto Artur Slompo Muniz Bicalho Marcelo de Vito Filho Daise Aparecida Rossi |
| author_facet | Phelipe Augusto Borba Martins Peres Roberta Torres de Melo Paulo Marcel Armendaris Fabiano Barreto Tiago Follmann Perin Ana Laura Grazziotin Guilherme Paz Monteiro Ana Beatriz Garcez Buiatte Eliane Pereira Mendonça Eduarda Cristina Alves Lourenzatto Artur Slompo Muniz Bicalho Marcelo de Vito Filho Daise Aparecida Rossi |
| author_sort | Phelipe Augusto Borba Martins Peres |
| collection | DOAJ |
| description | Campylobacter jejuni is the most frequent cause of bacterial gastroenteritis; therefore, the characteristics of its epidemiology must be continuously investigated to support possible mitigating measures. This is particularly important when evaluating representative strains from the world's leading chicken meat exporter, Brazil. We evaluated a panel of 14 virulence genes in 359 strains of C. jejuni isolated from chilled broiler carcasses in Brazil. The genes were classified into five virulence categories (B: biofilm/motility; SS: secretion/cytotoxicity system; CI: invasion/colonization; GB: Guillain-Barré; and AE: adaptation to stress). The percentage of strains with stress adaptation genes (86.07%) indicates the ability to survive in unfavorable environments; in addition, the strains showed a risk of causing infections in humans due to the frequency of the hcp gene (97.77%). Genes related to Guillain-Barre syndrome (GBS) in 77.44% of strains are an additional concern, which must be monitored. The gene panel showed the presence of 124 virulence profiles. Individual analyses by carcass, slaughter establishment, and municipalities in which they were located showed high index variabilities (I.Var.) of 0.82, 0.87, and 0.78, respectively. Georeferencing indicated the state of Paraná as a hotspot for virulent strains. Higher levels of isolation and multi-virulence were identified in the summer, which is hot and humid in Brazil. Together, our results showed that the studied strains are a potential danger to public health and that there is an urgent need for their surveillance and the adoption of control measures, especially in the state of Paraná. |
| first_indexed | 2024-03-12T17:34:47Z |
| format | Article |
| id | doaj.art-9eefa299dd4f413da9dcf752c8153e77 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T17:34:47Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9eefa299dd4f413da9dcf752c8153e772023-08-04T12:16:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12205791220579Multi-virulence of Campylobacter jejuni carried by chicken meat in BrazilPhelipe Augusto Borba Martins Peres0Roberta Torres de Melo1Paulo Marcel Armendaris2Fabiano Barreto3Tiago Follmann Perin4Ana Laura Grazziotin5Guilherme Paz Monteiro6Ana Beatriz Garcez Buiatte7Eliane Pereira Mendonça8Eduarda Cristina Alves Lourenzatto9Artur Slompo Muniz Bicalho10Marcelo de Vito Filho11Daise Aparecida Rossi12Laboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório Federal de Defesa Agropecuária/RS - LFDA/RS, Porto Alegre, Rio Grande do Sul, BrazilLaboratório Federal de Defesa Agropecuária/RS - LFDA/RS, Porto Alegre, Rio Grande do Sul, BrazilLaboratório Federal de Defesa Agropecuária/RS - LFDA/RS, Porto Alegre, Rio Grande do Sul, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilLaboratório de Epidemiologia Molecular - LEPIMOL/UFU, Uberlândia, Minas Gerais, BrazilCampylobacter jejuni is the most frequent cause of bacterial gastroenteritis; therefore, the characteristics of its epidemiology must be continuously investigated to support possible mitigating measures. This is particularly important when evaluating representative strains from the world's leading chicken meat exporter, Brazil. We evaluated a panel of 14 virulence genes in 359 strains of C. jejuni isolated from chilled broiler carcasses in Brazil. The genes were classified into five virulence categories (B: biofilm/motility; SS: secretion/cytotoxicity system; CI: invasion/colonization; GB: Guillain-Barré; and AE: adaptation to stress). The percentage of strains with stress adaptation genes (86.07%) indicates the ability to survive in unfavorable environments; in addition, the strains showed a risk of causing infections in humans due to the frequency of the hcp gene (97.77%). Genes related to Guillain-Barre syndrome (GBS) in 77.44% of strains are an additional concern, which must be monitored. The gene panel showed the presence of 124 virulence profiles. Individual analyses by carcass, slaughter establishment, and municipalities in which they were located showed high index variabilities (I.Var.) of 0.82, 0.87, and 0.78, respectively. Georeferencing indicated the state of Paraná as a hotspot for virulent strains. Higher levels of isolation and multi-virulence were identified in the summer, which is hot and humid in Brazil. Together, our results showed that the studied strains are a potential danger to public health and that there is an urgent need for their surveillance and the adoption of control measures, especially in the state of Paraná.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1220579/fullpoultry-farmpublic healthenvironmental adaptionmulti-virulenceMALDI-TOF |
| spellingShingle | Phelipe Augusto Borba Martins Peres Roberta Torres de Melo Paulo Marcel Armendaris Fabiano Barreto Tiago Follmann Perin Ana Laura Grazziotin Guilherme Paz Monteiro Ana Beatriz Garcez Buiatte Eliane Pereira Mendonça Eduarda Cristina Alves Lourenzatto Artur Slompo Muniz Bicalho Marcelo de Vito Filho Daise Aparecida Rossi Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil Frontiers in Microbiology poultry-farm public health environmental adaption multi-virulence MALDI-TOF |
| title | Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil |
| title_full | Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil |
| title_fullStr | Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil |
| title_full_unstemmed | Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil |
| title_short | Multi-virulence of Campylobacter jejuni carried by chicken meat in Brazil |
| title_sort | multi virulence of campylobacter jejuni carried by chicken meat in brazil |
| topic | poultry-farm public health environmental adaption multi-virulence MALDI-TOF |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1220579/full |
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