Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus
Infection leads to heightened activation of natural killer (NK) cells, a process that likely involves direct cell-to-cell contact, but how this occurs in vivo is poorly understood. We have used two-photon laser-scanning microscopy in conjunction with Toxoplasma gondii mouse infection models to addre...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712001611 |
| _version_ | 1811247319264264192 |
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| author | Janine L. Coombes Seong-Ji Han Nico van Rooijen David H. Raulet Ellen A. Robey |
| author_facet | Janine L. Coombes Seong-Ji Han Nico van Rooijen David H. Raulet Ellen A. Robey |
| author_sort | Janine L. Coombes |
| collection | DOAJ |
| description | Infection leads to heightened activation of natural killer (NK) cells, a process that likely involves direct cell-to-cell contact, but how this occurs in vivo is poorly understood. We have used two-photon laser-scanning microscopy in conjunction with Toxoplasma gondii mouse infection models to address this question. We found that after infection, NK cells accumulated in the subcapsular region of the lymph node, where they formed low-motility contacts with collagen fibers and CD169+ macrophages. We provide evidence that interactions with collagen regulate NK cell migration, whereas CD169+ macrophages increase the activation state of NK cells. Interestingly, a subset of CD169+ macrophages that coexpress the inflammatory monocyte marker Ly6C had the most potent ability to activate NK cells. Our data reveal pathways through which NK cell migration and function are regulated after infection and identify an important accessory cell population for activation of NK cell responses in lymph nodes. |
| first_indexed | 2024-04-12T15:06:50Z |
| format | Article |
| id | doaj.art-9ef28039681f4f828437b5996f73dc13 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-12T15:06:50Z |
| publishDate | 2012-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-9ef28039681f4f828437b5996f73dc132022-12-22T03:27:54ZengElsevierCell Reports2211-12472012-07-012112413510.1016/j.celrep.2012.06.001Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular SinusJanine L. Coombes0Seong-Ji Han1Nico van Rooijen2David H. Raulet3Ellen A. Robey4Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USADepartment of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USADepartment of Molecular Cell Biology, Vrije Universiteit, Vrije Universiteit Medical Center, Amsterdam 1081 BT, The NetherlandsDepartment of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USADepartment of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USAInfection leads to heightened activation of natural killer (NK) cells, a process that likely involves direct cell-to-cell contact, but how this occurs in vivo is poorly understood. We have used two-photon laser-scanning microscopy in conjunction with Toxoplasma gondii mouse infection models to address this question. We found that after infection, NK cells accumulated in the subcapsular region of the lymph node, where they formed low-motility contacts with collagen fibers and CD169+ macrophages. We provide evidence that interactions with collagen regulate NK cell migration, whereas CD169+ macrophages increase the activation state of NK cells. Interestingly, a subset of CD169+ macrophages that coexpress the inflammatory monocyte marker Ly6C had the most potent ability to activate NK cells. Our data reveal pathways through which NK cell migration and function are regulated after infection and identify an important accessory cell population for activation of NK cell responses in lymph nodes.http://www.sciencedirect.com/science/article/pii/S2211124712001611 |
| spellingShingle | Janine L. Coombes Seong-Ji Han Nico van Rooijen David H. Raulet Ellen A. Robey Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus Cell Reports |
| title | Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus |
| title_full | Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus |
| title_fullStr | Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus |
| title_full_unstemmed | Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus |
| title_short | Infection-Induced Regulation of Natural Killer Cells by Macrophages and Collagen at the Lymph Node Subcapsular Sinus |
| title_sort | infection induced regulation of natural killer cells by macrophages and collagen at the lymph node subcapsular sinus |
| url | http://www.sciencedirect.com/science/article/pii/S2211124712001611 |
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