Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA
Two populations of Astyanax altiparanae (Garutti et Britski, 2000) of the Água dos Patos stream/SP and lake Igapó/PR were analyzed. All individuals showed 2n = 50, however, different karyotypic formulae were observed. The population of the Água dos Patos stream showe...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2011-08-01
|
| Series: | Comparative Cytogenetics |
| Online Access: | http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1721 |
| _version_ | 1831546058187997184 |
|---|---|
| author | R. B. Pacheco Renata Rosa L. Giuliano-Caetano H. F. Júlio Jr. A. L. Dias |
| author_facet | R. B. Pacheco Renata Rosa L. Giuliano-Caetano H. F. Júlio Jr. A. L. Dias |
| author_sort | R. B. Pacheco |
| collection | DOAJ |
| description | Two populations of Astyanax altiparanae (Garutti et Britski, 2000) of the Água dos Patos stream/SP and lake Igapó/PR were analyzed. All individuals showed 2n = 50, however, different karyotypic formulae were observed. The population of the Água dos Patos stream showed 8m +24sm+6st+12a (NF=88) and the population of lake Igapó, 8m+28sm+4st+10a (NF=90). Nucleolus organizing regions (AgNORs) were observed in the terminal position on the short and long arm of different chromosomes of both populations, showing a variation from 3 to 4 chromosomes. Fluorescent in situ hybridization (FISH) using 18S rDNA probes revealed only one pair of chromosomes with fluorescent signals in the terminal site on the short arm in the Igapó lake population, while the population of Água dos Patos stream showed 4 fluorescence terminal signals, characterizing a system of simple and multiple NORs, respectively. 5S rDNA fluorescent signals were detected in the interstitial position of a pair of chromosomes in the two studied populations. Some AgNOR sites revealed to be GC-rich when stained with Chromomycin A3 (CMA3), however, AT positive regions were not observed. The data obtained show that, despite the conservation of the diploid number and location of 5S DNAr, differences in both the distribution of 18S rDNA and karyotypic formula among the populations were found, thus corroborating the existing data on chromosome variability in Astyanax altiparanae that can be significant for cytotaxonomy in this group. |
| first_indexed | 2024-12-17T01:37:55Z |
| format | Article |
| id | doaj.art-9ef43bb9e1ca4d63ad017da72a4ffc59 |
| institution | Directory Open Access Journal |
| issn | 1993-0771 1993-078X |
| language | English |
| last_indexed | 2024-12-17T01:37:55Z |
| publishDate | 2011-08-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Comparative Cytogenetics |
| spelling | doaj.art-9ef43bb9e1ca4d63ad017da72a4ffc592022-12-21T22:08:24ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2011-08-015323724610.3897/compcytogen.v5i3.12351721Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNAR. B. PachecoRenata RosaL. Giuliano-CaetanoH. F. Júlio Jr.A. L. DiasTwo populations of Astyanax altiparanae (Garutti et Britski, 2000) of the Água dos Patos stream/SP and lake Igapó/PR were analyzed. All individuals showed 2n = 50, however, different karyotypic formulae were observed. The population of the Água dos Patos stream showed 8m +24sm+6st+12a (NF=88) and the population of lake Igapó, 8m+28sm+4st+10a (NF=90). Nucleolus organizing regions (AgNORs) were observed in the terminal position on the short and long arm of different chromosomes of both populations, showing a variation from 3 to 4 chromosomes. Fluorescent in situ hybridization (FISH) using 18S rDNA probes revealed only one pair of chromosomes with fluorescent signals in the terminal site on the short arm in the Igapó lake population, while the population of Água dos Patos stream showed 4 fluorescence terminal signals, characterizing a system of simple and multiple NORs, respectively. 5S rDNA fluorescent signals were detected in the interstitial position of a pair of chromosomes in the two studied populations. Some AgNOR sites revealed to be GC-rich when stained with Chromomycin A3 (CMA3), however, AT positive regions were not observed. The data obtained show that, despite the conservation of the diploid number and location of 5S DNAr, differences in both the distribution of 18S rDNA and karyotypic formula among the populations were found, thus corroborating the existing data on chromosome variability in Astyanax altiparanae that can be significant for cytotaxonomy in this group.http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1721 |
| spellingShingle | R. B. Pacheco Renata Rosa L. Giuliano-Caetano H. F. Júlio Jr. A. L. Dias Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA Comparative Cytogenetics |
| title | Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA |
| title_full | Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA |
| title_fullStr | Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA |
| title_full_unstemmed | Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA |
| title_short | Cytogenetic comparison between two allopatric populations of Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae), with emphasis on the localization of 18S and 5S rDNA |
| title_sort | cytogenetic comparison between two allopatric populations of astyanax altiparanae garutti britski 2000 teleostei characidae with emphasis on the localization of 18s and 5s rdna |
| url | http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1721 |
| work_keys_str_mv | AT rbpacheco cytogeneticcomparisonbetweentwoallopatricpopulationsofastyanaxaltiparanaegaruttibritski2000teleosteicharacidaewithemphasisonthelocalizationof18sand5srdna AT renatarosa cytogeneticcomparisonbetweentwoallopatricpopulationsofastyanaxaltiparanaegaruttibritski2000teleosteicharacidaewithemphasisonthelocalizationof18sand5srdna AT lgiulianocaetano cytogeneticcomparisonbetweentwoallopatricpopulationsofastyanaxaltiparanaegaruttibritski2000teleosteicharacidaewithemphasisonthelocalizationof18sand5srdna AT hfjuliojr cytogeneticcomparisonbetweentwoallopatricpopulationsofastyanaxaltiparanaegaruttibritski2000teleosteicharacidaewithemphasisonthelocalizationof18sand5srdna AT aldias cytogeneticcomparisonbetweentwoallopatricpopulationsofastyanaxaltiparanaegaruttibritski2000teleosteicharacidaewithemphasisonthelocalizationof18sand5srdna |