Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas
<p>Nitrous oxide (N<span class="inline-formula"><sub>2</sub></span>O) is an important ozone-depleting greenhouse gas produced and consumed by microbially mediated nitrification and denitrification pathways. Estuaries are intensive N<span class="inline-...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2022-08-01
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| Series: | Biogeosciences |
| Online Access: | https://bg.copernicus.org/articles/19/3757/2022/bg-19-3757-2022.pdf |
| _version_ | 1817998696469495808 |
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| author | X. Dai M. Chen X. Wan E. Tan J. Zeng N. Chen N. Chen S.-J. Kao S.-J. Kao Y. Zhang |
| author_facet | X. Dai M. Chen X. Wan E. Tan J. Zeng N. Chen N. Chen S.-J. Kao S.-J. Kao Y. Zhang |
| author_sort | X. Dai |
| collection | DOAJ |
| description | <p>Nitrous oxide (N<span class="inline-formula"><sub>2</sub></span>O) is an important ozone-depleting
greenhouse gas produced and consumed by microbially mediated nitrification
and denitrification pathways. Estuaries are intensive N<span class="inline-formula"><sub>2</sub></span>O emission
regions in marine ecosystems. However, the potential contributions of
nitrifiers and denitrifiers to N<span class="inline-formula"><sub>2</sub></span>O sources and sinks in China's
estuarine and coastal areas are poorly understood. The abundance and
transcription of six key microbial functional genes involved in
nitrification and denitrification, as well as the clade II-type <i>nosZ</i>
gene-bearing community composition of N<span class="inline-formula"><sub>2</sub></span>O reducers, were investigated
in four estuaries spanning the Chinese coastline. The results showed that
the ammonia-oxidizing archaeal <i>amoA</i> genes and transcripts were more dominant in
the northern Bohai Sea (BS) and Yangtze River estuaries, which had low
nitrogen concentrations, while the denitrifier <i>nirS</i> genes and transcripts were
more dominant in the southern Jiulong River (JRE) and Pearl River estuaries,
which had high levels of terrestrial nitrogen input. Notably, the <i>nosZ</i> clade II
gene was more abundant than the clade I-type throughout the estuaries except
for in the JRE and a few sites of the BS, while the opposite transcript
distribution pattern was observed in these two estuaries. The gene and
transcript distributions were significantly constrained by nitrogen and
oxygen concentrations as well as by salinity, temperature, and pH. The <i>nosZ</i> clade
II gene-bearing community composition along China's coastline had a high level of
diversity and was distinctly different from that in the soil and in marine
oxygen-minimum-zone waters. By comparing the gene distribution patterns
across the estuaries with the distribution patterns of the N<span class="inline-formula"><sub>2</sub></span>O
concentration and flux, we found that denitrification may principally
control the N<span class="inline-formula"><sub>2</sub></span>O emissions pattern.</p> |
| first_indexed | 2024-04-14T02:57:09Z |
| format | Article |
| id | doaj.art-9efcf4edeca9407590d11c4e8a21e3b3 |
| institution | Directory Open Access Journal |
| issn | 1726-4170 1726-4189 |
| language | English |
| last_indexed | 2024-04-14T02:57:09Z |
| publishDate | 2022-08-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Biogeosciences |
| spelling | doaj.art-9efcf4edeca9407590d11c4e8a21e3b32022-12-22T02:16:03ZengCopernicus PublicationsBiogeosciences1726-41701726-41892022-08-01193757377310.5194/bg-19-3757-2022Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areasX. Dai0M. Chen1X. Wan2E. Tan3J. Zeng4N. Chen5N. Chen6S.-J. Kao7S.-J. Kao8Y. Zhang9State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, ChinaDepartment of Geosciences, Princeton University, Princeton, New Jersey 08544, USAState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan, 570228, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, ChinaFujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, College of the Environment and Ecology, Xiamen University, Xiamen 361005, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan, 570228, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China<p>Nitrous oxide (N<span class="inline-formula"><sub>2</sub></span>O) is an important ozone-depleting greenhouse gas produced and consumed by microbially mediated nitrification and denitrification pathways. Estuaries are intensive N<span class="inline-formula"><sub>2</sub></span>O emission regions in marine ecosystems. However, the potential contributions of nitrifiers and denitrifiers to N<span class="inline-formula"><sub>2</sub></span>O sources and sinks in China's estuarine and coastal areas are poorly understood. The abundance and transcription of six key microbial functional genes involved in nitrification and denitrification, as well as the clade II-type <i>nosZ</i> gene-bearing community composition of N<span class="inline-formula"><sub>2</sub></span>O reducers, were investigated in four estuaries spanning the Chinese coastline. The results showed that the ammonia-oxidizing archaeal <i>amoA</i> genes and transcripts were more dominant in the northern Bohai Sea (BS) and Yangtze River estuaries, which had low nitrogen concentrations, while the denitrifier <i>nirS</i> genes and transcripts were more dominant in the southern Jiulong River (JRE) and Pearl River estuaries, which had high levels of terrestrial nitrogen input. Notably, the <i>nosZ</i> clade II gene was more abundant than the clade I-type throughout the estuaries except for in the JRE and a few sites of the BS, while the opposite transcript distribution pattern was observed in these two estuaries. The gene and transcript distributions were significantly constrained by nitrogen and oxygen concentrations as well as by salinity, temperature, and pH. The <i>nosZ</i> clade II gene-bearing community composition along China's coastline had a high level of diversity and was distinctly different from that in the soil and in marine oxygen-minimum-zone waters. By comparing the gene distribution patterns across the estuaries with the distribution patterns of the N<span class="inline-formula"><sub>2</sub></span>O concentration and flux, we found that denitrification may principally control the N<span class="inline-formula"><sub>2</sub></span>O emissions pattern.</p>https://bg.copernicus.org/articles/19/3757/2022/bg-19-3757-2022.pdf |
| spellingShingle | X. Dai M. Chen X. Wan E. Tan J. Zeng N. Chen N. Chen S.-J. Kao S.-J. Kao Y. Zhang Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas Biogeosciences |
| title | Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas |
| title_full | Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas |
| title_fullStr | Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas |
| title_full_unstemmed | Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas |
| title_short | Potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in China's estuarine and coastal areas |
| title_sort | potential contributions of nitrifiers and denitrifiers to nitrous oxide sources and sinks in china s estuarine and coastal areas |
| url | https://bg.copernicus.org/articles/19/3757/2022/bg-19-3757-2022.pdf |
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