Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels
Mitochondrial pyruvate carrier 1 (MPC1) is a component of the MPC1/MPC2 heterodimer that facilitates the transport of pyruvate into mitochondria. Pyruvate plays a central role in carbohydrate, fatty, and amino acid catabolism. The present study examined epididymal white adipose tissue (eWAT) and int...
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| Format: | Article |
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PeerJ Inc.
2018-10-01
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| Online Access: | https://peerj.com/articles/5799.pdf |
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| author | Shiying Zou Liye Zhu Kunlun Huang Haoshu Luo Wentao Xu Xiaoyun He |
| author_facet | Shiying Zou Liye Zhu Kunlun Huang Haoshu Luo Wentao Xu Xiaoyun He |
| author_sort | Shiying Zou |
| collection | DOAJ |
| description | Mitochondrial pyruvate carrier 1 (MPC1) is a component of the MPC1/MPC2 heterodimer that facilitates the transport of pyruvate into mitochondria. Pyruvate plays a central role in carbohydrate, fatty, and amino acid catabolism. The present study examined epididymal white adipose tissue (eWAT) and intrascapular brown adipose tissue (iBAT) from MPC1± mice following 24 weeks of feeding, which indicated low energy accumulation as evidenced by low body and eWAT weight and adipocyte volume. To characterize molecular changes in energy metabolism, we analyzed the transcriptomes of the adipose tissues using RNA-Sequencing (RNA-Seq). The results showed that the fatty acid oxidation pathway was activated and several genes involved in this pathway were upregulated. Furthermore, qPCR and western blotting indicated that numerous genes and proteins that participate in lipolysis were also upregulated. Based on these findings, we propose that the energy deficiency caused by reduced MPC1 activity can be alleviated by activating the lipolytic pathway. |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:36:22Z |
| publishDate | 2018-10-01 |
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| spelling | doaj.art-9efd9a32013d4277a98a291dcbe9b7c12023-12-03T10:58:18ZengPeerJ Inc.PeerJ2167-83592018-10-016e579910.7717/peerj.5799Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levelsShiying Zou0Liye Zhu1Kunlun Huang2Haoshu Luo3Wentao Xu4Xiaoyun He5China Agricultural University, Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, Beijing, ChinaChina Agricultural University, Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, Beijing, ChinaChina Agricultural University, Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, Beijing, ChinaChina Agricultural University, College of Biological Sciences, Beijing, ChinaChina Agricultural University, Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, Beijing, ChinaChina Agricultural University, Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, Beijing, ChinaMitochondrial pyruvate carrier 1 (MPC1) is a component of the MPC1/MPC2 heterodimer that facilitates the transport of pyruvate into mitochondria. Pyruvate plays a central role in carbohydrate, fatty, and amino acid catabolism. The present study examined epididymal white adipose tissue (eWAT) and intrascapular brown adipose tissue (iBAT) from MPC1± mice following 24 weeks of feeding, which indicated low energy accumulation as evidenced by low body and eWAT weight and adipocyte volume. To characterize molecular changes in energy metabolism, we analyzed the transcriptomes of the adipose tissues using RNA-Sequencing (RNA-Seq). The results showed that the fatty acid oxidation pathway was activated and several genes involved in this pathway were upregulated. Furthermore, qPCR and western blotting indicated that numerous genes and proteins that participate in lipolysis were also upregulated. Based on these findings, we propose that the energy deficiency caused by reduced MPC1 activity can be alleviated by activating the lipolytic pathway.https://peerj.com/articles/5799.pdfMitochondrial pyruvate carrier 1RNA-SequencingWhite adipose tissuePyruvateEnergy metabolism |
| spellingShingle | Shiying Zou Liye Zhu Kunlun Huang Haoshu Luo Wentao Xu Xiaoyun He Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels PeerJ Mitochondrial pyruvate carrier 1 RNA-Sequencing White adipose tissue Pyruvate Energy metabolism |
| title | Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels |
| title_full | Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels |
| title_fullStr | Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels |
| title_full_unstemmed | Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels |
| title_short | Adipose tissues of MPC1± mice display altered lipid metabolism-related enzyme expression levels |
| title_sort | adipose tissues of mpc1 mice display altered lipid metabolism related enzyme expression levels |
| topic | Mitochondrial pyruvate carrier 1 RNA-Sequencing White adipose tissue Pyruvate Energy metabolism |
| url | https://peerj.com/articles/5799.pdf |
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