Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario
ABSTRACT Introduction: cholelithiasis is a highly prevalent disease of the digestive system in the world. In Brazil, it is a routine condition, whose studies suggest a prevalence of around 10% of adults. Colonization of bile and gallstone pathogens can occur when there is bacterial stasis and prol...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Cirurgiões
2023-04-01
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| Series: | Revista do Colégio Brasileiro de Cirurgiões |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912023000100216&tlng=pt |
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| author | BRUNO HENRIQUE NUNES HIRATA SUZETHE SASAGAWA ALESSANDRA NAVARINI HENRIQUE CUNHA MATEUS ADHEMAR MONTEIRO PACHECO JUNIOR MAURO JOSÉ COSTA SALLES |
| author_facet | BRUNO HENRIQUE NUNES HIRATA SUZETHE SASAGAWA ALESSANDRA NAVARINI HENRIQUE CUNHA MATEUS ADHEMAR MONTEIRO PACHECO JUNIOR MAURO JOSÉ COSTA SALLES |
| author_sort | BRUNO HENRIQUE NUNES HIRATA |
| collection | DOAJ |
| description | ABSTRACT Introduction: cholelithiasis is a highly prevalent disease of the digestive system in the world. In Brazil, it is a routine condition, whose studies suggest a prevalence of around 10% of adults. Colonization of bile and gallstone pathogens can occur when there is bacterial stasis and proliferation. This proliferation is facilitated by the adhesion and biofilm formation capacity of some bacteria. There are also lithogenic processes that involve bacterial participation. Studies have shown changes in the microbiota of the gallbladder of patients undergoing cholecystectomy, which may impact empirical treatment with antibiotics. Methodology: microbiological analyzes of the sonication fluid of the gallstones and of two samples with bile were performed. Identification and antimicrobial susceptibility testing were performed according to a standard routine. Results: of the 34 patients, 76.4% were female. The age group was 48 years +/- 16.61. Acute cholecystitis occurred in 50% of cases. Bactobilia was evidenced in 32.1% of the cases. Klebisiella pneumoniae was noted as the most prevalent pathogen in acute cholecystitis; and Enterobacter sp, in cases of uncomplicated cholelithiasis. Greater sensitivity was obtained in the search for microorganisms in the sonication fluid samples of the stones in relation to the bile samples (p=0.0058). Conclusion: there was a higher prevalence of bactobilia in patients with acute cholecystitis compared to those with uncomplicated cholelithiasis. The use of sonication in bacterial investigation proved to be superior to the conventional method and can be considered. |
| first_indexed | 2024-04-09T13:42:54Z |
| format | Article |
| id | doaj.art-9efe559518d54d0b86e682652779fe2f |
| institution | Directory Open Access Journal |
| issn | 1809-4546 |
| language | English |
| last_indexed | 2024-04-09T13:42:54Z |
| publishDate | 2023-04-01 |
| publisher | Colégio Brasileiro de Cirurgiões |
| record_format | Article |
| series | Revista do Colégio Brasileiro de Cirurgiões |
| spelling | doaj.art-9efe559518d54d0b86e682652779fe2f2023-05-09T07:31:30ZengColégio Brasileiro de CirurgiõesRevista do Colégio Brasileiro de Cirurgiões1809-45462023-04-015010.1590/0100-6991e-20233474-enComparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenarioBRUNO HENRIQUE NUNES HIRATAhttps://orcid.org/0000-0003-4552-4927SUZETHE SASAGAWAhttps://orcid.org/0000-0002-5191-1061ALESSANDRA NAVARINIhttps://orcid.org/0000-0002-8656-8291HENRIQUE CUNHA MATEUShttps://orcid.org/0000-0001-7040-3022ADHEMAR MONTEIRO PACHECO JUNIORhttps://orcid.org/0000-0001-8773-8785MAURO JOSÉ COSTA SALLEShttps://orcid.org/0000-0002-5443-6024ABSTRACT Introduction: cholelithiasis is a highly prevalent disease of the digestive system in the world. In Brazil, it is a routine condition, whose studies suggest a prevalence of around 10% of adults. Colonization of bile and gallstone pathogens can occur when there is bacterial stasis and proliferation. This proliferation is facilitated by the adhesion and biofilm formation capacity of some bacteria. There are also lithogenic processes that involve bacterial participation. Studies have shown changes in the microbiota of the gallbladder of patients undergoing cholecystectomy, which may impact empirical treatment with antibiotics. Methodology: microbiological analyzes of the sonication fluid of the gallstones and of two samples with bile were performed. Identification and antimicrobial susceptibility testing were performed according to a standard routine. Results: of the 34 patients, 76.4% were female. The age group was 48 years +/- 16.61. Acute cholecystitis occurred in 50% of cases. Bactobilia was evidenced in 32.1% of the cases. Klebisiella pneumoniae was noted as the most prevalent pathogen in acute cholecystitis; and Enterobacter sp, in cases of uncomplicated cholelithiasis. Greater sensitivity was obtained in the search for microorganisms in the sonication fluid samples of the stones in relation to the bile samples (p=0.0058). Conclusion: there was a higher prevalence of bactobilia in patients with acute cholecystitis compared to those with uncomplicated cholelithiasis. The use of sonication in bacterial investigation proved to be superior to the conventional method and can be considered.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912023000100216&tlng=ptMicrobiologyCholecystectomyCholecystitis, AcuteCholelithiasis |
| spellingShingle | BRUNO HENRIQUE NUNES HIRATA SUZETHE SASAGAWA ALESSANDRA NAVARINI HENRIQUE CUNHA MATEUS ADHEMAR MONTEIRO PACHECO JUNIOR MAURO JOSÉ COSTA SALLES Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario Revista do Colégio Brasileiro de Cirurgiões Microbiology Cholecystectomy Cholecystitis, Acute Cholelithiasis |
| title | Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario |
| title_full | Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario |
| title_fullStr | Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario |
| title_full_unstemmed | Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario |
| title_short | Comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis: changes in the epidemiological scenario |
| title_sort | comparison of bacterial profile of gallbladder with gallstones from patients undergoing cholecystectomy due to complicated and uncomplicated cholelithiasis changes in the epidemiological scenario |
| topic | Microbiology Cholecystectomy Cholecystitis, Acute Cholelithiasis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69912023000100216&tlng=pt |
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