Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus
The aim of this study was to examine the absorption of fucoidan through the intestinal tract. Fucoidan (0.1, 0.5, 1.0, 1.5 and 2.0 mg/mL) was added to Transwell inserts containing Caco-2 cells. The transport of fucoidan across Caco-2 cells increased in a dose-dependent manner up to 1.0 mg/mL. It rea...
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MDPI AG
2014-12-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/1/48 |
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| author | Takeaki Nagamine Kyoumi Nakazato Satoru Tomioka Masahiko Iha Katsuyuki Nakajima |
| author_facet | Takeaki Nagamine Kyoumi Nakazato Satoru Tomioka Masahiko Iha Katsuyuki Nakajima |
| author_sort | Takeaki Nagamine |
| collection | DOAJ |
| description | The aim of this study was to examine the absorption of fucoidan through the intestinal tract. Fucoidan (0.1, 0.5, 1.0, 1.5 and 2.0 mg/mL) was added to Transwell inserts containing Caco-2 cells. The transport of fucoidan across Caco-2 cells increased in a dose-dependent manner up to 1.0 mg/mL. It reached a maximum after 1 h and then rapidly decreased. In another experiment, rats were fed standard chow containing 2% fucoidan for one or two weeks. Immunohistochemical staining revealed that fucoidan accumulated in jejunal epithelial cells, mononuclear cells in the jejunal lamina propria and sinusoidal non-parenchymal cells in the liver. Since we previously speculated that nitrosamine may enhance the intestinal absorption of fucoidan, its absorption was estimated in rats administered N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in their drinking water. Rats were fed 0.2% fucoidan chow (BBN + 0.2% fucoidan rats), 2% fucoidan chow (BBN + 2% fucoidan rats) and standard chow for eight weeks. The uptake of fucoidan through the intestinal tract seemed to be low, but was measurable by our ELISA method. Fucoidan-positive cells were abundant in the small intestinal mucosa of BBN + 2% fucoidan rats. Most fucoidan-positive cells also stained positive for ED1, suggesting that fucoidan was incorporated into intestinal macrophages. The uptake of fucoidan by Kupffer cells was observed in the livers of BBN + 2% fucoidan rats. In conclusion, the absorption of fucoidan through the small intestine was demonstrated both in vivo and in vitro. |
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| language | English |
| last_indexed | 2024-04-11T12:18:26Z |
| publishDate | 2014-12-01 |
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| series | Marine Drugs |
| spelling | doaj.art-9eff08e80e614e53b3018e5e5d8e2bb72022-12-22T04:24:11ZengMDPI AGMarine Drugs1660-33972014-12-01131486410.3390/md13010048md13010048Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranusTakeaki Nagamine0Kyoumi Nakazato1Satoru Tomioka2Masahiko Iha3Katsuyuki Nakajima4Graduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, JapanGraduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, JapanGraduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, JapanSouth Product, Co., Ltd., Uruma, Okinawa 904-1103, JapanGraduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, JapanThe aim of this study was to examine the absorption of fucoidan through the intestinal tract. Fucoidan (0.1, 0.5, 1.0, 1.5 and 2.0 mg/mL) was added to Transwell inserts containing Caco-2 cells. The transport of fucoidan across Caco-2 cells increased in a dose-dependent manner up to 1.0 mg/mL. It reached a maximum after 1 h and then rapidly decreased. In another experiment, rats were fed standard chow containing 2% fucoidan for one or two weeks. Immunohistochemical staining revealed that fucoidan accumulated in jejunal epithelial cells, mononuclear cells in the jejunal lamina propria and sinusoidal non-parenchymal cells in the liver. Since we previously speculated that nitrosamine may enhance the intestinal absorption of fucoidan, its absorption was estimated in rats administered N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in their drinking water. Rats were fed 0.2% fucoidan chow (BBN + 0.2% fucoidan rats), 2% fucoidan chow (BBN + 2% fucoidan rats) and standard chow for eight weeks. The uptake of fucoidan through the intestinal tract seemed to be low, but was measurable by our ELISA method. Fucoidan-positive cells were abundant in the small intestinal mucosa of BBN + 2% fucoidan rats. Most fucoidan-positive cells also stained positive for ED1, suggesting that fucoidan was incorporated into intestinal macrophages. The uptake of fucoidan by Kupffer cells was observed in the livers of BBN + 2% fucoidan rats. In conclusion, the absorption of fucoidan through the small intestine was demonstrated both in vivo and in vitro.http://www.mdpi.com/1660-3397/13/1/48fucoidanintestinal absorptionmacrophageKupffer cellsCaco-2 cells |
| spellingShingle | Takeaki Nagamine Kyoumi Nakazato Satoru Tomioka Masahiko Iha Katsuyuki Nakajima Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus Marine Drugs fucoidan intestinal absorption macrophage Kupffer cells Caco-2 cells |
| title | Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus |
| title_full | Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus |
| title_fullStr | Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus |
| title_full_unstemmed | Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus |
| title_short | Intestinal Absorption of Fucoidan Extracted from the Brown Seaweed, Cladosiphon okamuranus |
| title_sort | intestinal absorption of fucoidan extracted from the brown seaweed cladosiphon okamuranus |
| topic | fucoidan intestinal absorption macrophage Kupffer cells Caco-2 cells |
| url | http://www.mdpi.com/1660-3397/13/1/48 |
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