Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin
Waste from marine fish processing is an important source of valuable products. Fish collagen is considered a alternative biomaterial due to its excellent properties, and it is widely used for industrial purposes. Thus, this present study aimed to characterize acid and pepsin-soluble collagens from t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2023-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2023/7312447 |
| _version_ | 1797833702798000128 |
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| author | Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda |
| author_facet | Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda |
| author_sort | Abdul Aziz Jaziri |
| collection | DOAJ |
| description | Waste from marine fish processing is an important source of valuable products. Fish collagen is considered a alternative biomaterial due to its excellent properties, and it is widely used for industrial purposes. Thus, this present study aimed to characterize acid and pepsin-soluble collagens from the waste of parrotfish (Scarus sordidus Forsskål, 1775) scales. The yields (p > 0.05) of acid-soluble collagen (ASC-PFS) and pepsin-soluble collagen (PSC-PFS) were 1.17 g/100 g and 1.00 g/100 g, respectively. Both collagen samples were categorized as type I owing to the presence of two alpha chain subunits (α1 and α1) after being confirmed by a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Under the fourier transform infrared (FTIR) test, the triple helical structure of type I collagens from the ASC-PFS and PSC-PFS was maintained. Moreover, the study of UV visible spectra and X-ray diffraction (XRD) showed the similarity of collagens derived from different fish species, and the thermostability (Tmax) evaluation of all extracted collagens was in the range of 36.22–37.78°C, and their values were comparable to previous research on the fish scale collagens. The effect of various pH and sodium chloride (NaCl) treatments on solubility exhibited that the ASC-PFS and PSC-PFS were highly soluble in an acidic condition (pH < 5.0) and low concentration of sodium chloride (<30 g/L). Taken together, collagens extracted from parrotfish scale waste can be an alternative source for industries. |
| first_indexed | 2024-04-09T14:28:28Z |
| format | Article |
| id | doaj.art-9eff34501e66406ba5aa0035f15e0f06 |
| institution | Directory Open Access Journal |
| issn | 1687-8795 |
| language | English |
| last_indexed | 2024-04-09T14:28:28Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj.art-9eff34501e66406ba5aa0035f15e0f062023-05-04T00:00:05ZengHindawi LimitedInternational Journal of Biomaterials1687-87952023-01-01202310.1155/2023/7312447Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and PepsinAbdul Aziz Jaziri0Rossita Shapawi1Ruzaidi Azli Mohd Mokhtar2Wan Norhana Md. Noordin3Nurul Huda4Faculty of Food Science and NutritionBorneo Marine Research InstituteBiotechnology Research InstituteFisheries Research InstituteFaculty of Sustainable AgricultureWaste from marine fish processing is an important source of valuable products. Fish collagen is considered a alternative biomaterial due to its excellent properties, and it is widely used for industrial purposes. Thus, this present study aimed to characterize acid and pepsin-soluble collagens from the waste of parrotfish (Scarus sordidus Forsskål, 1775) scales. The yields (p > 0.05) of acid-soluble collagen (ASC-PFS) and pepsin-soluble collagen (PSC-PFS) were 1.17 g/100 g and 1.00 g/100 g, respectively. Both collagen samples were categorized as type I owing to the presence of two alpha chain subunits (α1 and α1) after being confirmed by a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Under the fourier transform infrared (FTIR) test, the triple helical structure of type I collagens from the ASC-PFS and PSC-PFS was maintained. Moreover, the study of UV visible spectra and X-ray diffraction (XRD) showed the similarity of collagens derived from different fish species, and the thermostability (Tmax) evaluation of all extracted collagens was in the range of 36.22–37.78°C, and their values were comparable to previous research on the fish scale collagens. The effect of various pH and sodium chloride (NaCl) treatments on solubility exhibited that the ASC-PFS and PSC-PFS were highly soluble in an acidic condition (pH < 5.0) and low concentration of sodium chloride (<30 g/L). Taken together, collagens extracted from parrotfish scale waste can be an alternative source for industries.http://dx.doi.org/10.1155/2023/7312447 |
| spellingShingle | Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin International Journal of Biomaterials |
| title | Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin |
| title_full | Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin |
| title_fullStr | Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin |
| title_full_unstemmed | Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin |
| title_short | Extraction and Characterization of Type I Collagen from Parrotfish (Scarus sordidus Forsskål, 1775) Scale solubilized with the Aid of Acetic Acid and Pepsin |
| title_sort | extraction and characterization of type i collagen from parrotfish scarus sordidus forsskal 1775 scale solubilized with the aid of acetic acid and pepsin |
| url | http://dx.doi.org/10.1155/2023/7312447 |
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