Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts
<b> </b>This study<b> </b>was conducted to compare the effects of acute hyperthermia (45 °C for 4 h) on the viability, proliferation, and migratory activity through wound-healing assays of cow and sheep fibroblasts. The study examined the effects on primary cultures...
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MDPI AG
2020-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/545 |
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| author | Islam M. Saadeldin Ayman Abdel-Aziz Swelum Adel M. Zakri Hammed A. Tukur Abdullah N. Alowaimer |
| author_facet | Islam M. Saadeldin Ayman Abdel-Aziz Swelum Adel M. Zakri Hammed A. Tukur Abdullah N. Alowaimer |
| author_sort | Islam M. Saadeldin |
| collection | DOAJ |
| description | <b> </b>This study<b> </b>was conducted to compare the effects of acute hyperthermia (45 °C for 4 h) on the viability, proliferation, and migratory activity through wound-healing assays of cow and sheep fibroblasts. The study examined the effects on primary cultures and first passage skin-derived fibroblasts. Relative quantification of HSP70, HSP90, P53, BAX, BCL2, and BECN1 was investigated after normalization to housekeeping genes GAPDH and beta-actin. The results revealed that cultured cow primary fibroblasts exhibited increased viability and reinitiated cell migration to close the cell monolayer scratch earlier than sheep cells. Similar patterns were observed in the first passage fibroblasts, with severe effects on sheep cells. Both cow and sheep cells exhibited decreased cell viability and failed to regain migratory activity after re-exposure of recovered heat-shocked cells. Effects of hyperthermia on sheep cells were potentiated by cell cryopreservation. The qPCR results showed that cow cells significantly increased HSP70 and HSP90 expression, which decreased the elevation of P53, and ameliorated the effects of the increased BAX/BCL2 ratio. The results provide a paradigm to compare thermotolerance among different animal species and revealed that trypsin could be an additional stress, which potentiates the effects of heat shock in in vitro experiments. |
| first_indexed | 2024-12-11T05:53:00Z |
| format | Article |
| id | doaj.art-9eff9fe7026f4d6b8ac2d6dc9ac2cfb2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-11T05:53:00Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9eff9fe7026f4d6b8ac2d6dc9ac2cfb22022-12-22T01:18:45ZengMDPI AGAnimals2076-26152020-03-0110454510.3390/ani10040545ani10040545Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived FibroblastsIslam M. Saadeldin0Ayman Abdel-Aziz Swelum1Adel M. Zakri2Hammed A. Tukur3Abdullah N. Alowaimer4Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaPlant Protection Department, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia<b> </b>This study<b> </b>was conducted to compare the effects of acute hyperthermia (45 °C for 4 h) on the viability, proliferation, and migratory activity through wound-healing assays of cow and sheep fibroblasts. The study examined the effects on primary cultures and first passage skin-derived fibroblasts. Relative quantification of HSP70, HSP90, P53, BAX, BCL2, and BECN1 was investigated after normalization to housekeeping genes GAPDH and beta-actin. The results revealed that cultured cow primary fibroblasts exhibited increased viability and reinitiated cell migration to close the cell monolayer scratch earlier than sheep cells. Similar patterns were observed in the first passage fibroblasts, with severe effects on sheep cells. Both cow and sheep cells exhibited decreased cell viability and failed to regain migratory activity after re-exposure of recovered heat-shocked cells. Effects of hyperthermia on sheep cells were potentiated by cell cryopreservation. The qPCR results showed that cow cells significantly increased HSP70 and HSP90 expression, which decreased the elevation of P53, and ameliorated the effects of the increased BAX/BCL2 ratio. The results provide a paradigm to compare thermotolerance among different animal species and revealed that trypsin could be an additional stress, which potentiates the effects of heat shock in in vitro experiments.https://www.mdpi.com/2076-2615/10/4/545fibroblastshyperthermiathermotoleranceheat shock proteinscowssheep |
| spellingShingle | Islam M. Saadeldin Ayman Abdel-Aziz Swelum Adel M. Zakri Hammed A. Tukur Abdullah N. Alowaimer Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts Animals fibroblasts hyperthermia thermotolerance heat shock proteins cows sheep |
| title | Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts |
| title_full | Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts |
| title_fullStr | Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts |
| title_full_unstemmed | Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts |
| title_short | Effects of Acute Hyperthermia on the Thermotolerance of Cow and Sheep Skin-Derived Fibroblasts |
| title_sort | effects of acute hyperthermia on the thermotolerance of cow and sheep skin derived fibroblasts |
| topic | fibroblasts hyperthermia thermotolerance heat shock proteins cows sheep |
| url | https://www.mdpi.com/2076-2615/10/4/545 |
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