Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets
One way to improve the growth of low-birth-weight (LBW) piglets can be stimulation of the cellular development of muscle by optimized amino acid supply. In the current study, it was investigated how glutamine (Gln) supplementation affects muscle tissue of LBW and normal-birth-weight (NBW) piglets. L...
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MDPI AG
2023-02-01
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| Series: | Cells |
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| author | Elke Albrecht Yaolu Zhao Quentin L. Sciascia Cornelia C. Metges Steffen Maak |
| author_facet | Elke Albrecht Yaolu Zhao Quentin L. Sciascia Cornelia C. Metges Steffen Maak |
| author_sort | Elke Albrecht |
| collection | DOAJ |
| description | One way to improve the growth of low-birth-weight (LBW) piglets can be stimulation of the cellular development of muscle by optimized amino acid supply. In the current study, it was investigated how glutamine (Gln) supplementation affects muscle tissue of LBW and normal-birth-weight (NBW) piglets. Longissimus and semitendinosus muscles of 96 male piglets, which were supplemented with 1 g Gln/kg body weight or alanine, were collected at slaughter on day 5 or 26 post natum (dpn), one hour after injection with Bromodeoxyuridine (BrdU, 12 mg/kg). Immunohistochemistry was applied to detect proliferating, BrdU-positive cells in muscle cross-sections. Serial stainings with cell type specific antibodies enabled detection and subsequent quantification of proliferating satellite cells and identification of further proliferating cell types, e.g., preadipocytes and immune cells. The results indicated that satellite cells and macrophages comprise the largest fractions of proliferating cells in skeletal muscle of piglets early after birth. The Gln supplementation somewhat stimulated satellite cells. We observed differences between the two muscles, but no influence of the piglets’ birth weight was observed. Thus, Gln supplements may not be considered as effective treatment in piglets with low birth weight for improvement of muscle growth. |
| first_indexed | 2024-03-11T09:01:07Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T09:01:07Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj.art-9f023fade68246d0a3d869afb15b43042023-11-16T19:44:19ZengMDPI AGCells2073-44092023-02-0112458010.3390/cells12040580Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight PigletsElke Albrecht0Yaolu Zhao1Quentin L. Sciascia2Cornelia C. Metges3Steffen Maak4Research Institute for Farm Animal Biology (FBN), Institute of Muscle Biology and Growth, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Muscle Biology and Growth, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology “Oskar Kellner”, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology “Oskar Kellner”, 18196 Dummerstorf, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Muscle Biology and Growth, 18196 Dummerstorf, GermanyOne way to improve the growth of low-birth-weight (LBW) piglets can be stimulation of the cellular development of muscle by optimized amino acid supply. In the current study, it was investigated how glutamine (Gln) supplementation affects muscle tissue of LBW and normal-birth-weight (NBW) piglets. Longissimus and semitendinosus muscles of 96 male piglets, which were supplemented with 1 g Gln/kg body weight or alanine, were collected at slaughter on day 5 or 26 post natum (dpn), one hour after injection with Bromodeoxyuridine (BrdU, 12 mg/kg). Immunohistochemistry was applied to detect proliferating, BrdU-positive cells in muscle cross-sections. Serial stainings with cell type specific antibodies enabled detection and subsequent quantification of proliferating satellite cells and identification of further proliferating cell types, e.g., preadipocytes and immune cells. The results indicated that satellite cells and macrophages comprise the largest fractions of proliferating cells in skeletal muscle of piglets early after birth. The Gln supplementation somewhat stimulated satellite cells. We observed differences between the two muscles, but no influence of the piglets’ birth weight was observed. Thus, Gln supplements may not be considered as effective treatment in piglets with low birth weight for improvement of muscle growth.https://www.mdpi.com/2073-4409/12/4/580muscle satellite cellBrdUPAX7preadipocyteDLK1immunohistochemistry |
| spellingShingle | Elke Albrecht Yaolu Zhao Quentin L. Sciascia Cornelia C. Metges Steffen Maak Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets Cells muscle satellite cell BrdU PAX7 preadipocyte DLK1 immunohistochemistry |
| title | Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets |
| title_full | Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets |
| title_fullStr | Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets |
| title_full_unstemmed | Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets |
| title_short | Identification and Quantification of Proliferating Cells in Skeletal Muscle of Glutamine Supplemented Low- and Normal-Birth-Weight Piglets |
| title_sort | identification and quantification of proliferating cells in skeletal muscle of glutamine supplemented low and normal birth weight piglets |
| topic | muscle satellite cell BrdU PAX7 preadipocyte DLK1 immunohistochemistry |
| url | https://www.mdpi.com/2073-4409/12/4/580 |
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