Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt
Noura E Esmaeel, 1 Marian A Gerges, 1 Thoraya A Hosny, 2 Ahmed R Ali, 3 Manar G Gebriel 1 1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 3Urology Dep...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-02-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/detection-of-chromosomal-and-plasmid-mediated-quinolone-resistance-amo-peer-reviewed-article-IDR |
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| author | Esmaeel NE Gerges MA Hosny TA Ali AR Gebriel MG |
| author_facet | Esmaeel NE Gerges MA Hosny TA Ali AR Gebriel MG |
| author_sort | Esmaeel NE |
| collection | DOAJ |
| description | Noura E Esmaeel, 1 Marian A Gerges, 1 Thoraya A Hosny, 2 Ahmed R Ali, 3 Manar G Gebriel 1 1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 3Urology Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptCorrespondence: Marian A GergesMedical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, 44519, EgyptTel +20 1003819530Email maromicro2006@yahoo.comIntroduction: Resistance to fluoroquinolones (FQ) in uropathogenic Escherichia coli (UPEC) has emerged as a growing problem. Chromosomal mutations and plasmid-mediated quinolone resistance (PMQR) determinants have been implicated. Data concerning the prevalence of these determinants in UPEC in our hospital are quite limited.Purpose: To investigate the occurrence and genetic determinants of FQ resistance in UPEC isolated from urinary tract infection (UTI) cases in Zagazig University Hospitals.Patients and Methods: Following their isolation, the identification and susceptibility of UPEC isolates were performed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS). FQ resistance was detected by the disc diffusion method. Ciprofloxacin minimal inhibitory concentration (MIC) was determined using E-test. Chromosomal mutations in the gyrA gene were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and for detection of PMQR, a couple of multiplex PCR reactions were used.Results: Among a total of 192 UPEC isolates, 46.9% (n=90) were FQ resistant. More than half of the isolates (57.8%) exhibited high-level ciprofloxacin resistance (MIC > 32 μg/mL). Mutations in gyrA were detected in 76.7% of isolates, with 34.4% having mutations at more than one site. PMQR determinants were detected in 80.1% of UPEC isolates, with aac(6ʹ)-Ib-cr gene being the most frequent found in 61.1% of isolates.Conclusion: There is a high prevalence of both gyrA mutations and PMQR determinants among UPEC isolates in our hospital which contribute to high-level ciprofloxacin resistance, a finding that may require the revision of the antibiotics used for empirical treatment of UTI.Keywords: gyrA mutations, qnr determinants, uropathogenic E. coli, ciprofloxacin resistance |
| first_indexed | 2024-12-22T00:34:22Z |
| format | Article |
| id | doaj.art-9f03734ada48480ab54025254ec001e2 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-22T00:34:22Z |
| publishDate | 2020-02-01 |
| publisher | Dove Medical Press |
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| series | Infection and Drug Resistance |
| spelling | doaj.art-9f03734ada48480ab54025254ec001e22022-12-21T18:44:52ZengDove Medical PressInfection and Drug Resistance1178-69732020-02-01Volume 1341342151723Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, EgyptEsmaeel NEGerges MAHosny TAAli ARGebriel MGNoura E Esmaeel, 1 Marian A Gerges, 1 Thoraya A Hosny, 2 Ahmed R Ali, 3 Manar G Gebriel 1 1Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 3Urology Department, Faculty of Medicine, Zagazig University, Zagazig, EgyptCorrespondence: Marian A GergesMedical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, 44519, EgyptTel +20 1003819530Email maromicro2006@yahoo.comIntroduction: Resistance to fluoroquinolones (FQ) in uropathogenic Escherichia coli (UPEC) has emerged as a growing problem. Chromosomal mutations and plasmid-mediated quinolone resistance (PMQR) determinants have been implicated. Data concerning the prevalence of these determinants in UPEC in our hospital are quite limited.Purpose: To investigate the occurrence and genetic determinants of FQ resistance in UPEC isolated from urinary tract infection (UTI) cases in Zagazig University Hospitals.Patients and Methods: Following their isolation, the identification and susceptibility of UPEC isolates were performed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS). FQ resistance was detected by the disc diffusion method. Ciprofloxacin minimal inhibitory concentration (MIC) was determined using E-test. Chromosomal mutations in the gyrA gene were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and for detection of PMQR, a couple of multiplex PCR reactions were used.Results: Among a total of 192 UPEC isolates, 46.9% (n=90) were FQ resistant. More than half of the isolates (57.8%) exhibited high-level ciprofloxacin resistance (MIC > 32 μg/mL). Mutations in gyrA were detected in 76.7% of isolates, with 34.4% having mutations at more than one site. PMQR determinants were detected in 80.1% of UPEC isolates, with aac(6ʹ)-Ib-cr gene being the most frequent found in 61.1% of isolates.Conclusion: There is a high prevalence of both gyrA mutations and PMQR determinants among UPEC isolates in our hospital which contribute to high-level ciprofloxacin resistance, a finding that may require the revision of the antibiotics used for empirical treatment of UTI.Keywords: gyrA mutations, qnr determinants, uropathogenic E. coli, ciprofloxacin resistancehttps://www.dovepress.com/detection-of-chromosomal-and-plasmid-mediated-quinolone-resistance-amo-peer-reviewed-article-IDRgyra mutationsqnr determinantsuropathogenic e. coliciprofloxacin resistance |
| spellingShingle | Esmaeel NE Gerges MA Hosny TA Ali AR Gebriel MG Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt Infection and Drug Resistance gyra mutations qnr determinants uropathogenic e. coli ciprofloxacin resistance |
| title | Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt |
| title_full | Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt |
| title_fullStr | Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt |
| title_full_unstemmed | Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt |
| title_short | Detection of Chromosomal and Plasmid-Mediated Quinolone Resistance Among Escherichia coli Isolated from Urinary Tract Infection Cases; Zagazig University Hospitals, Egypt |
| title_sort | detection of chromosomal and plasmid mediated quinolone resistance among escherichia coli isolated from urinary tract infection cases zagazig university hospitals egypt |
| topic | gyra mutations qnr determinants uropathogenic e. coli ciprofloxacin resistance |
| url | https://www.dovepress.com/detection-of-chromosomal-and-plasmid-mediated-quinolone-resistance-amo-peer-reviewed-article-IDR |
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