Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR
Toxoplasma gondii is a unique intracellular parasite, which infect a large proportion of the world, population, but clinically un commonly causes significant disease. The present study was performed for the first time in Thi-Qar province to estimate the prevalence of toxoplasmosis among university...
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| Format: | Article |
| Language: | English |
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University of Thi-Qar
2019-04-01
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| Series: | مجلة علوم ذي قار |
| Subjects: | |
| Online Access: | http://jsci.utq.edu.iq/index.php/main/article/view/106 |
| _version_ | 1797404192456835072 |
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| author | Rabab Al-Mosawi |
| author_facet | Rabab Al-Mosawi |
| author_sort | Rabab Al-Mosawi |
| collection | DOAJ |
| description |
Toxoplasma gondii is a unique intracellular parasite, which infect a large proportion of the world, population, but
clinically un commonly causes significant disease. The present study was performed for the first time in Thi-Qar
province to estimate the prevalence of toxoplasmosis among university students . Venous blood samples were collected
from 319 (111 males and 208 females) apparently healthy students ,they have ages between (18-42) years attended from
different colleges of Thi-Qar university-Iraq, during the period from October 2013 to April 2014. Enzyme linked
Immunosorbant Assay (ELISA), was used to evaluate the presence of anti-Toxoplasma IgM and IgG antibodies and
detection of B1 gene of T.gondii DNA by Real-Time Polymerase Chain Reaction(RT-PCR). The results indicated that
70 (21.94%) of students were exposed positive for anti-Toxoplasma antibodies,17 (5.33%) of them had IgM,38
(11.91%) had IgG, and 15 (4.70%) had both IgM and IgG. Statistically, significant difference between them. The
results showed significant difference between males and females in rate of positive for anti- Toxoplasma antibodies,
which recorded 24 (7.52%) and 46 (14.42%) respectively . Besides the serological diagnosis of T.gondii Real-Time PCR
(RT-PCR) technique was used to confirm the infection with T.gondii by detection B1 gene of T.gondii DNA in the
blood of students. out of 319 students only 6.26% showed positive toxoplasmosis among those 8(2.50%)were males
and 12(3.76%) were females. The positive result in RT-PCR analysis were distributed on the patterns of the antiToxoplasma
antibodies by ELISA test , it was no found any positive blood samples with IgM,IgG and both IgM and
IgG respectively whereas 20 positive cases of no anti –Toxoplasma antibodies. Real-Time PCR test in blood of students
has advantages in detection of recent or active toxoplasmosis.
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| first_indexed | 2024-03-09T02:51:22Z |
| format | Article |
| id | doaj.art-9f05a505ba374f61af5df004e203a894 |
| institution | Directory Open Access Journal |
| issn | 1991-8690 2709-0256 |
| language | English |
| last_indexed | 2024-03-09T02:51:22Z |
| publishDate | 2019-04-01 |
| publisher | University of Thi-Qar |
| record_format | Article |
| series | مجلة علوم ذي قار |
| spelling | doaj.art-9f05a505ba374f61af5df004e203a8942023-12-05T09:58:11ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-04-0152Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCRRabab Al-Mosawi Toxoplasma gondii is a unique intracellular parasite, which infect a large proportion of the world, population, but clinically un commonly causes significant disease. The present study was performed for the first time in Thi-Qar province to estimate the prevalence of toxoplasmosis among university students . Venous blood samples were collected from 319 (111 males and 208 females) apparently healthy students ,they have ages between (18-42) years attended from different colleges of Thi-Qar university-Iraq, during the period from October 2013 to April 2014. Enzyme linked Immunosorbant Assay (ELISA), was used to evaluate the presence of anti-Toxoplasma IgM and IgG antibodies and detection of B1 gene of T.gondii DNA by Real-Time Polymerase Chain Reaction(RT-PCR). The results indicated that 70 (21.94%) of students were exposed positive for anti-Toxoplasma antibodies,17 (5.33%) of them had IgM,38 (11.91%) had IgG, and 15 (4.70%) had both IgM and IgG. Statistically, significant difference between them. The results showed significant difference between males and females in rate of positive for anti- Toxoplasma antibodies, which recorded 24 (7.52%) and 46 (14.42%) respectively . Besides the serological diagnosis of T.gondii Real-Time PCR (RT-PCR) technique was used to confirm the infection with T.gondii by detection B1 gene of T.gondii DNA in the blood of students. out of 319 students only 6.26% showed positive toxoplasmosis among those 8(2.50%)were males and 12(3.76%) were females. The positive result in RT-PCR analysis were distributed on the patterns of the antiToxoplasma antibodies by ELISA test , it was no found any positive blood samples with IgM,IgG and both IgM and IgG respectively whereas 20 positive cases of no anti –Toxoplasma antibodies. Real-Time PCR test in blood of students has advantages in detection of recent or active toxoplasmosis. http://jsci.utq.edu.iq/index.php/main/article/view/106Toxoplasma gondiiReal-Time PCRB1 gene |
| spellingShingle | Rabab Al-Mosawi Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR مجلة علوم ذي قار Toxoplasma gondii Real-Time PCR B1 gene |
| title | Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR |
| title_full | Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR |
| title_fullStr | Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR |
| title_full_unstemmed | Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR |
| title_short | Diagnostic study of Toxoplasma gondii in students of Thi-Qar university-Iraq by Real- Time PCR |
| title_sort | diagnostic study of toxoplasma gondii in students of thi qar university iraq by real time pcr |
| topic | Toxoplasma gondii Real-Time PCR B1 gene |
| url | http://jsci.utq.edu.iq/index.php/main/article/view/106 |
| work_keys_str_mv | AT rababalmosawi diagnosticstudyoftoxoplasmagondiiinstudentsofthiqaruniversityiraqbyrealtimepcr |