Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency
Abstract Nutrient deficiency alters growth and the production of high-quality nutritious food. In Citrus crops, rootstock technologies have become a key tool for enhancing tolerance to abiotic stress. The use of doubled diploid rootstocks can improve adaptation to lower nutrient inputs. This study i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-88383-5 |
| _version_ | 1830144032846643200 |
|---|---|
| author | Julie Oustric Stéphane Herbette Yann Quilichini Raphaël Morillon Jean Giannettini Liliane Berti Jérémie Santini |
| author_facet | Julie Oustric Stéphane Herbette Yann Quilichini Raphaël Morillon Jean Giannettini Liliane Berti Jérémie Santini |
| author_sort | Julie Oustric |
| collection | DOAJ |
| description | Abstract Nutrient deficiency alters growth and the production of high-quality nutritious food. In Citrus crops, rootstock technologies have become a key tool for enhancing tolerance to abiotic stress. The use of doubled diploid rootstocks can improve adaptation to lower nutrient inputs. This study investigated leaf structure and ultrastructure and physiological and biochemical parameters of diploid common clementine scions (C) grafted on diploid (2x) and doubled diploid (4x) Carrizo citrange (C/CC2x and C/CC4x) and Citrumelo 4475 (C/CM2x and C/CM4x) rootstocks under optimal fertigation and after 7 months of nutrient deficiency. Rootstock ploidy level had no impact on structure but induced changes in the number and/or size of cells and some cell components of 2x common clementine leaves under optimal nutrition. Rootstock ploidy level did not modify gas exchanges in Carrizo citrange but induced a reduction in the leaf net photosynthetic rate in Citrumelo 4475. By assessing foliar damage, changes in photosynthetic processes and malondialdehyde accumulation, we found that C/CM4x were less affected by nutrient deficiency than the other scion/rootstock combinations. Their greater tolerance to nutrient deficiency was probably due to the better performance of the enzyme-based antioxidant system. Nutrient deficiency had similar impacts on C/CC2x and C/CC4x. Tolerance to nutrient deficiency can therefore be improved by rootstock polyploidy but remains dependent on the rootstock genotype. |
| first_indexed | 2024-12-17T09:59:08Z |
| format | Article |
| id | doaj.art-9f06b28212834fb59edccd20079589c7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T09:59:08Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9f06b28212834fb59edccd20079589c72022-12-21T21:53:20ZengNature PortfolioScientific Reports2045-23222021-04-0111111510.1038/s41598-021-88383-5Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiencyJulie Oustric0Stéphane Herbette1Yann Quilichini2Raphaël Morillon3Jean Giannettini4Liliane Berti5Jérémie Santini6CNRS, Équipe de Biochimie et Biologie Moléculaire du Végétal, UMR 6134 SPE, Université de CorseUCA, INRAE, PIAFCNRS, Équipe des Parasites et Ecosystèmes Méditerranéens, UMR 6134 SPE, Université de CorseEquipe SEAPAG, CIRAD, UMR AGAPCNRS, Équipe de Biochimie et Biologie Moléculaire du Végétal, UMR 6134 SPE, Université de CorseCNRS, Équipe de Biochimie et Biologie Moléculaire du Végétal, UMR 6134 SPE, Université de CorseCNRS, Équipe de Biochimie et Biologie Moléculaire du Végétal, UMR 6134 SPE, Université de CorseAbstract Nutrient deficiency alters growth and the production of high-quality nutritious food. In Citrus crops, rootstock technologies have become a key tool for enhancing tolerance to abiotic stress. The use of doubled diploid rootstocks can improve adaptation to lower nutrient inputs. This study investigated leaf structure and ultrastructure and physiological and biochemical parameters of diploid common clementine scions (C) grafted on diploid (2x) and doubled diploid (4x) Carrizo citrange (C/CC2x and C/CC4x) and Citrumelo 4475 (C/CM2x and C/CM4x) rootstocks under optimal fertigation and after 7 months of nutrient deficiency. Rootstock ploidy level had no impact on structure but induced changes in the number and/or size of cells and some cell components of 2x common clementine leaves under optimal nutrition. Rootstock ploidy level did not modify gas exchanges in Carrizo citrange but induced a reduction in the leaf net photosynthetic rate in Citrumelo 4475. By assessing foliar damage, changes in photosynthetic processes and malondialdehyde accumulation, we found that C/CM4x were less affected by nutrient deficiency than the other scion/rootstock combinations. Their greater tolerance to nutrient deficiency was probably due to the better performance of the enzyme-based antioxidant system. Nutrient deficiency had similar impacts on C/CC2x and C/CC4x. Tolerance to nutrient deficiency can therefore be improved by rootstock polyploidy but remains dependent on the rootstock genotype.https://doi.org/10.1038/s41598-021-88383-5 |
| spellingShingle | Julie Oustric Stéphane Herbette Yann Quilichini Raphaël Morillon Jean Giannettini Liliane Berti Jérémie Santini Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency Scientific Reports |
| title | Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency |
| title_full | Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency |
| title_fullStr | Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency |
| title_full_unstemmed | Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency |
| title_short | Tetraploid Citrumelo 4475 rootstocks improve diploid common clementine tolerance to long-term nutrient deficiency |
| title_sort | tetraploid citrumelo 4475 rootstocks improve diploid common clementine tolerance to long term nutrient deficiency |
| url | https://doi.org/10.1038/s41598-021-88383-5 |
| work_keys_str_mv | AT julieoustric tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency AT stephaneherbette tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency AT yannquilichini tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency AT raphaelmorillon tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency AT jeangiannettini tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency AT lilianeberti tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency AT jeremiesantini tetraploidcitrumelo4475rootstocksimprovediploidcommonclementinetolerancetolongtermnutrientdeficiency |