A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS
<p align="left">The spectrum of proteins was studied in plerocercoids of parasitic cestodes <em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Triaenophorus </span></em>nodulosus <span style="font-f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Karelian Research Centre of the Russian Academy of Sciences
2018-12-01
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| Series: | Transactions of the Karelian Research Centre of the Russian Academy of Sciences |
| Subjects: | |
| Online Access: | http://journals.krc.karelia.ru/index.php/biology/article/view/899 |
| _version_ | 1819140109746831360 |
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| author | Albina Kochneva Ekaterina Borvinskaya Darya Bedulina Irina Sukhovskaya |
| author_facet | Albina Kochneva Ekaterina Borvinskaya Darya Bedulina Irina Sukhovskaya |
| author_sort | Albina Kochneva |
| collection | DOAJ |
| description | <p align="left">The spectrum of proteins was studied in plerocercoids of parasitic cestodes <em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Triaenophorus </span></em>nodulosus <span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">collected from the liver of different species of fish: perch </span></span><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Perca fluviatilis </span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">L</span></span><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">.,</span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"> ruffe </span>Gymnocephalus cernuus <span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">L. and burbot </span></span><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Lota lota </span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">L. A comparison of protein extracts separated </span>by 2D electrophoresis revealed 18 proteins whose concentrations differed among larvae of different fish species. Of these, 4 proteins were identified by using mass-spectrometric methods (MALDI-TOF/TOF, LC–MS): a carbohydrate metabolism enzyme triosephosphate isomerase, the proteins of microtubules tubulin α and β, and the G-protein signaling molecule. The elevated content of components of the G-protein pathway of the cytoskeleton and cell morphology regulation (tubulin and G-protein) in plerocercoids from perch in comparison with plerocercoids from burbot indicate a more dynamic cytoskeleton, increased cell proliferation and differentiation processes, and possibly more mature larvae in the former species. A higher level of expression of the key enzyme of glycolysis and signal transmission in plerocercoids from perch compared to plerocercoids from burbot indicates a greater metabolic activity in larvae from perch. The obtained results indicate that the expression of some proteins in <em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">T. nodulosus </span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">larvae is influenced </span>by the habitat. </p><p align="left"> </p> |
| first_indexed | 2024-12-22T11:33:20Z |
| format | Article |
| id | doaj.art-9f0702bd9bc348308d9d1b369439bd90 |
| institution | Directory Open Access Journal |
| issn | 1997-3217 2312-4504 |
| language | English |
| last_indexed | 2024-12-22T11:33:20Z |
| publishDate | 2018-12-01 |
| publisher | Karelian Research Centre of the Russian Academy of Sciences |
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| series | Transactions of the Karelian Research Centre of the Russian Academy of Sciences |
| spelling | doaj.art-9f0702bd9bc348308d9d1b369439bd902022-12-21T18:27:31ZengKarelian Research Centre of the Russian Academy of SciencesTransactions of the Karelian Research Centre of the Russian Academy of Sciences1997-32172312-45042018-12-011210.17076/eb899667A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTSAlbina Kochneva0Ekaterina Borvinskaya1Darya Bedulina2Irina Sukhovskaya3Institute of Biology, Karelian Research Centre, Russian Academy of SciencesInstitute of Biology, Karelian Research Centre, Russian Academy of SciencesInstitute of Biology at Irkutsk State UniversityInstitute of Biology, Karelian Research Centre, Russian Academy of Sciences<p align="left">The spectrum of proteins was studied in plerocercoids of parasitic cestodes <em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Triaenophorus </span></em>nodulosus <span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">collected from the liver of different species of fish: perch </span></span><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Perca fluviatilis </span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">L</span></span><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">.,</span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"> ruffe </span>Gymnocephalus cernuus <span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA"><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">L. and burbot </span></span><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">Lota lota </span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">L. A comparison of protein extracts separated </span>by 2D electrophoresis revealed 18 proteins whose concentrations differed among larvae of different fish species. Of these, 4 proteins were identified by using mass-spectrometric methods (MALDI-TOF/TOF, LC–MS): a carbohydrate metabolism enzyme triosephosphate isomerase, the proteins of microtubules tubulin α and β, and the G-protein signaling molecule. The elevated content of components of the G-protein pathway of the cytoskeleton and cell morphology regulation (tubulin and G-protein) in plerocercoids from perch in comparison with plerocercoids from burbot indicate a more dynamic cytoskeleton, increased cell proliferation and differentiation processes, and possibly more mature larvae in the former species. A higher level of expression of the key enzyme of glycolysis and signal transmission in plerocercoids from perch compared to plerocercoids from burbot indicates a greater metabolic activity in larvae from perch. The obtained results indicate that the expression of some proteins in <em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA"><em><span style="font-family: PragmaticaBook-Italic; font-size: xx-small;" lang="JA">T. nodulosus </span></em></span></em><span style="font-family: PragmaticaBook-Reg; font-size: xx-small;" lang="JA">larvae is influenced </span>by the habitat. </p><p align="left"> </p>http://journals.krc.karelia.ru/index.php/biology/article/view/899proteomicshelminthstriaenophoruscestodaintermediate hosts. |
| spellingShingle | Albina Kochneva Ekaterina Borvinskaya Darya Bedulina Irina Sukhovskaya A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS Transactions of the Karelian Research Centre of the Russian Academy of Sciences proteomics helminths triaenophorus cestoda intermediate hosts. |
| title | A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS |
| title_full | A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS |
| title_fullStr | A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS |
| title_full_unstemmed | A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS |
| title_short | A COMPARISON OF THE PROTEIN PROFILE OF TRIAENOPHORUS NODULOSUS PLEROCERCOIDS FROM DIFFERENT INTERMEDIATE HOSTS |
| title_sort | comparison of the protein profile of triaenophorus nodulosus plerocercoids from different intermediate hosts |
| topic | proteomics helminths triaenophorus cestoda intermediate hosts. |
| url | http://journals.krc.karelia.ru/index.php/biology/article/view/899 |
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