Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan
The study was performed to provide an overview of the molecular epidemiology of carbapenem-resistant <i>Acinetobacter baumannii</i> in Afghanistan isolated by the German military medical service during the Afghanistan conflict. A total of 18 isolates were collected between 2012 and 2018...
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2229 |
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| author | Paul G. Higgins Meret Kniel Sandra Rojak Carsten Balczun Holger Rohde Hagen Frickmann Ralf Matthias Hagen |
| author_facet | Paul G. Higgins Meret Kniel Sandra Rojak Carsten Balczun Holger Rohde Hagen Frickmann Ralf Matthias Hagen |
| author_sort | Paul G. Higgins |
| collection | DOAJ |
| description | The study was performed to provide an overview of the molecular epidemiology of carbapenem-resistant <i>Acinetobacter baumannii</i> in Afghanistan isolated by the German military medical service during the Afghanistan conflict. A total of 18 isolates were collected between 2012 and 2018 at the microbiological laboratory of the field hospital in Camp Marmal near Mazar-e Sharif, Afghanistan, from Afghan patients. The isolates were subjected to phenotypic and genotypic differentiation and antimicrobial susceptibility testing as well as to a core genome multi-locus sequence typing (cgMLST) approach based on whole-genome next-generation sequence (wgNGS) data. Next to several sporadic isolates, four transmission clusters comprising strains from the international clonal lineages IC1, IC2, and IC9 were identified. Acquired carbapenem resistance was due to <i>bla</i><sub>OXA-23</sub> in 17/18 isolates, while genes mediating resistance against sulfonamides, macrolides, tetracyclines, and aminoglycosides were frequently identified as well. In conclusion, the assessment confirmed both the frequent occurrence of <i>A. baumannii</i> associated with outbreak events and a variety of different clones in Afghanistan. The fact that acquired carbapenem resistance was almost exclusively associated with <i>bla</i><sub>OXA-23</sub> may facilitate molecular resistance screening based on rapid molecular assays targeting this resistance determinant. |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:16:00Z |
| publishDate | 2021-10-01 |
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| series | Microorganisms |
| spelling | doaj.art-9f0734c7ae8542d18a63f65637e7da112023-11-23T00:28:17ZengMDPI AGMicroorganisms2076-26072021-10-01911222910.3390/microorganisms9112229Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, AfghanistanPaul G. Higgins0Meret Kniel1Sandra Rojak2Carsten Balczun3Holger Rohde4Hagen Frickmann5Ralf Matthias Hagen6Institute for Medical Microbiology, Immunology, and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50935 Cologne, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), 20251 Hamburg, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, GermanyDepartment of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, GermanyThe study was performed to provide an overview of the molecular epidemiology of carbapenem-resistant <i>Acinetobacter baumannii</i> in Afghanistan isolated by the German military medical service during the Afghanistan conflict. A total of 18 isolates were collected between 2012 and 2018 at the microbiological laboratory of the field hospital in Camp Marmal near Mazar-e Sharif, Afghanistan, from Afghan patients. The isolates were subjected to phenotypic and genotypic differentiation and antimicrobial susceptibility testing as well as to a core genome multi-locus sequence typing (cgMLST) approach based on whole-genome next-generation sequence (wgNGS) data. Next to several sporadic isolates, four transmission clusters comprising strains from the international clonal lineages IC1, IC2, and IC9 were identified. Acquired carbapenem resistance was due to <i>bla</i><sub>OXA-23</sub> in 17/18 isolates, while genes mediating resistance against sulfonamides, macrolides, tetracyclines, and aminoglycosides were frequently identified as well. In conclusion, the assessment confirmed both the frequent occurrence of <i>A. baumannii</i> associated with outbreak events and a variety of different clones in Afghanistan. The fact that acquired carbapenem resistance was almost exclusively associated with <i>bla</i><sub>OXA-23</sub> may facilitate molecular resistance screening based on rapid molecular assays targeting this resistance determinant.https://www.mdpi.com/2076-2607/9/11/2229<i>Acinetobacter baumannii</i>molecular epidemiologycarbapenem resistanceAfghanistansurveillance |
| spellingShingle | Paul G. Higgins Meret Kniel Sandra Rojak Carsten Balczun Holger Rohde Hagen Frickmann Ralf Matthias Hagen Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan Microorganisms <i>Acinetobacter baumannii</i> molecular epidemiology carbapenem resistance Afghanistan surveillance |
| title | Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan |
| title_full | Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan |
| title_fullStr | Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan |
| title_full_unstemmed | Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan |
| title_short | Molecular Epidemiology of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Strains Isolated at the German Military Field Laboratory in Mazar-e Sharif, Afghanistan |
| title_sort | molecular epidemiology of carbapenem resistant i acinetobacter baumannii i strains isolated at the german military field laboratory in mazar e sharif afghanistan |
| topic | <i>Acinetobacter baumannii</i> molecular epidemiology carbapenem resistance Afghanistan surveillance |
| url | https://www.mdpi.com/2076-2607/9/11/2229 |
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