Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis
Results from previous studies regarding platelet function in liver cirrhosis are discordant. The aim was to investigate platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis. We included 27 patients with alcoholic liver cirrhosis and 22 healthy individuals. A recent...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-07-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2017.1349308 |
| _version_ | 1797684282464927744 |
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| author | Pernille Just Vinholt Anne-Mette Hvas Christian Nielsen Anna Cecilia Söderström Ulrik Sprogøe Annette Dam Fialla Mads Nybo |
| author_facet | Pernille Just Vinholt Anne-Mette Hvas Christian Nielsen Anna Cecilia Söderström Ulrik Sprogøe Annette Dam Fialla Mads Nybo |
| author_sort | Pernille Just Vinholt |
| collection | DOAJ |
| description | Results from previous studies regarding platelet function in liver cirrhosis are discordant. The aim was to investigate platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis. We included 27 patients with alcoholic liver cirrhosis and 22 healthy individuals. A recently established flow cytometric approach was used to measure platelet activation and platelet aggregation independent of sample platelet count. Platelet aggregation was further investigated using light transmission aggregometry (LTA) (for platelet count >100 × 109/L). Platelet agonists were adenosine diphosphate, thrombin receptor-activating peptide, arachidonic acid, collagen, and collagen-related peptide. Patients had lower median platelet count than healthy individuals, 125 × 109/L (interquartile range [IQR] 90˗185) versus 240 × 109 (IQR 204˗285), p < 0.001. Platelet activation levels in stimulated samples were lower in patients versus healthy individuals, e.g., after collagen-related peptide stimulation, the median percentage of platelets positive for activated glycoprotein IIb/IIIa was 85% (IQR 70–94) in patients versus 97% (IQR 94–99) in healthy individuals, p < 0.001; lower platelet activation capacity being associated with low platelet count and Child–Pugh class B/C cirrhosis. Flow cytometric platelet aggregation was reduced in patients for collagen-related peptide and for adenosine diphosphate, e.g., platelet aggregation (mean ± standard deviation) was 57% ± 4 in patients versus 70% ± 1 in healthy individuals for collagen-related peptide, p = 0.01. Light LTA showed reduced collagen-induced platelet aggregation in some patients compared with healthy individuals. In conclusion, platelet function was reduced in some patients with alcoholic liver cirrhosis and the severity was associated with platelet count and severity of liver cirrhosis. |
| first_indexed | 2024-03-12T00:27:12Z |
| format | Article |
| id | doaj.art-9f07ee84071245d4ab07ca0a475bdb70 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:12Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-9f07ee84071245d4ab07ca0a475bdb702023-09-15T10:31:58ZengTaylor & Francis GroupPlatelets0953-71041369-16352018-07-0129552052710.1080/09537104.2017.13493081349308Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosisPernille Just Vinholt0Anne-Mette Hvas1Christian Nielsen2Anna Cecilia Söderström3Ulrik Sprogøe4Annette Dam Fialla5Mads Nybo6Odense University HospitalAarhus University HospitalOdense University HospitalOdense University HospitalOdense University HospitalOdense University HospitalOdense University HospitalResults from previous studies regarding platelet function in liver cirrhosis are discordant. The aim was to investigate platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis. We included 27 patients with alcoholic liver cirrhosis and 22 healthy individuals. A recently established flow cytometric approach was used to measure platelet activation and platelet aggregation independent of sample platelet count. Platelet aggregation was further investigated using light transmission aggregometry (LTA) (for platelet count >100 × 109/L). Platelet agonists were adenosine diphosphate, thrombin receptor-activating peptide, arachidonic acid, collagen, and collagen-related peptide. Patients had lower median platelet count than healthy individuals, 125 × 109/L (interquartile range [IQR] 90˗185) versus 240 × 109 (IQR 204˗285), p < 0.001. Platelet activation levels in stimulated samples were lower in patients versus healthy individuals, e.g., after collagen-related peptide stimulation, the median percentage of platelets positive for activated glycoprotein IIb/IIIa was 85% (IQR 70–94) in patients versus 97% (IQR 94–99) in healthy individuals, p < 0.001; lower platelet activation capacity being associated with low platelet count and Child–Pugh class B/C cirrhosis. Flow cytometric platelet aggregation was reduced in patients for collagen-related peptide and for adenosine diphosphate, e.g., platelet aggregation (mean ± standard deviation) was 57% ± 4 in patients versus 70% ± 1 in healthy individuals for collagen-related peptide, p = 0.01. Light LTA showed reduced collagen-induced platelet aggregation in some patients compared with healthy individuals. In conclusion, platelet function was reduced in some patients with alcoholic liver cirrhosis and the severity was associated with platelet count and severity of liver cirrhosis.http://dx.doi.org/10.1080/09537104.2017.1349308alcoholliver cirrhosisplatelet functionplatelet count |
| spellingShingle | Pernille Just Vinholt Anne-Mette Hvas Christian Nielsen Anna Cecilia Söderström Ulrik Sprogøe Annette Dam Fialla Mads Nybo Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis Platelets alcohol liver cirrhosis platelet function platelet count |
| title | Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis |
| title_full | Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis |
| title_fullStr | Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis |
| title_full_unstemmed | Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis |
| title_short | Reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis |
| title_sort | reduced platelet activation and platelet aggregation in patients with alcoholic liver cirrhosis |
| topic | alcohol liver cirrhosis platelet function platelet count |
| url | http://dx.doi.org/10.1080/09537104.2017.1349308 |
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