Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development
The combination of induced pluripotent stem cell (iPSC) technology and 3D cell culture creates a unique possibility for the generation of organoids that mimic human organs in in vitro cultures. The use of iPS cells in organoid cultures enables the differentiation of cells into dopaminergic neurons,...
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/17/5959 |
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| author | Anna Tejchman Agnieszka Znój Paula Chlebanowska Aneta Frączek-Szczypta Marcin Majka |
| author_facet | Anna Tejchman Agnieszka Znój Paula Chlebanowska Aneta Frączek-Szczypta Marcin Majka |
| author_sort | Anna Tejchman |
| collection | DOAJ |
| description | The combination of induced pluripotent stem cell (iPSC) technology and 3D cell culture creates a unique possibility for the generation of organoids that mimic human organs in in vitro cultures. The use of iPS cells in organoid cultures enables the differentiation of cells into dopaminergic neurons, also found in the human midbrain. However, long-lasting organoid cultures often cause necrosis within organoids. In this work, we present carbon fibers (CFs) for medical use as a new type of scaffold for organoid culture, comparing them to a previously tested copolymer poly-(lactic-<i>co</i>-glycolic acid) (PLGA) scaffold. We verified the physicochemical properties of CF scaffolds compared to PLGA in improving the efficiency of iPSC differentiation within organoids. The physicochemical properties of carbon scaffolds such as porosity, microstructure, or stability in the cellular environment make them a convenient material for creating in vitro organoid models. Through screening several genes expressed during the differentiation of organoids at crucial brain stages of development, we found that there is a correlation between <i>PITX3</i>, one of the key regulators of terminal differentiation, and the survival of midbrain dopaminergic (mDA) neurons and tyrosine hydroxylase (<i>TH</i>) gene expression. This makes organoids formed on carbon scaffolds an improved model containing mDA neurons convenient for studying midbrain-associated neurodegenerative diseases such as Parkinson’s disease. |
| first_indexed | 2024-03-10T17:11:17Z |
| format | Article |
| id | doaj.art-9f08544b6d1f4794ad2189c94e3bc69b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:11:17Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9f08544b6d1f4794ad2189c94e3bc69b2023-11-20T10:38:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117595910.3390/ijms21175959Carbon Fibers as a New Type of Scaffold for Midbrain Organoid DevelopmentAnna Tejchman0Agnieszka Znój1Paula Chlebanowska2Aneta Frączek-Szczypta3Marcin Majka4Department of Transplantation, Faculty of Medicine, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka 265, 30-663 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Transplantation, Faculty of Medicine, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka 265, 30-663 Kraków, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, PolandDepartment of Transplantation, Faculty of Medicine, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka 265, 30-663 Kraków, PolandThe combination of induced pluripotent stem cell (iPSC) technology and 3D cell culture creates a unique possibility for the generation of organoids that mimic human organs in in vitro cultures. The use of iPS cells in organoid cultures enables the differentiation of cells into dopaminergic neurons, also found in the human midbrain. However, long-lasting organoid cultures often cause necrosis within organoids. In this work, we present carbon fibers (CFs) for medical use as a new type of scaffold for organoid culture, comparing them to a previously tested copolymer poly-(lactic-<i>co</i>-glycolic acid) (PLGA) scaffold. We verified the physicochemical properties of CF scaffolds compared to PLGA in improving the efficiency of iPSC differentiation within organoids. The physicochemical properties of carbon scaffolds such as porosity, microstructure, or stability in the cellular environment make them a convenient material for creating in vitro organoid models. Through screening several genes expressed during the differentiation of organoids at crucial brain stages of development, we found that there is a correlation between <i>PITX3</i>, one of the key regulators of terminal differentiation, and the survival of midbrain dopaminergic (mDA) neurons and tyrosine hydroxylase (<i>TH</i>) gene expression. This makes organoids formed on carbon scaffolds an improved model containing mDA neurons convenient for studying midbrain-associated neurodegenerative diseases such as Parkinson’s disease.https://www.mdpi.com/1422-0067/21/17/5959organoidParkinson’s diseasecarbon fibersscaffold3D modelNURR1 |
| spellingShingle | Anna Tejchman Agnieszka Znój Paula Chlebanowska Aneta Frączek-Szczypta Marcin Majka Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development International Journal of Molecular Sciences organoid Parkinson’s disease carbon fibers scaffold 3D model NURR1 |
| title | Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development |
| title_full | Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development |
| title_fullStr | Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development |
| title_full_unstemmed | Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development |
| title_short | Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development |
| title_sort | carbon fibers as a new type of scaffold for midbrain organoid development |
| topic | organoid Parkinson’s disease carbon fibers scaffold 3D model NURR1 |
| url | https://www.mdpi.com/1422-0067/21/17/5959 |
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