The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product
The six <i>Dioscorea</i> species, <i>D. brevipetiolata</i>, <i>D. bulbifera</i>, <i>D. depauperata</i> (<i>Dd</i>), <i>D. glabra</i> (<i>Dg</i>), <i>D. pyrifolia</i> and <i>D. hamiltonii</i> w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/8/1551 |
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| author | Warin Wonok Arunrat Chaveerach Pornnarong Siripiyasing Runglawan Sudmoon Tawatchai Tanee |
| author_facet | Warin Wonok Arunrat Chaveerach Pornnarong Siripiyasing Runglawan Sudmoon Tawatchai Tanee |
| author_sort | Warin Wonok |
| collection | DOAJ |
| description | The six <i>Dioscorea</i> species, <i>D. brevipetiolata</i>, <i>D. bulbifera</i>, <i>D. depauperata</i> (<i>Dd</i>), <i>D. glabra</i> (<i>Dg</i>), <i>D. pyrifolia</i> and <i>D. hamiltonii</i> were analyzed for phytochemicals, toxicity in PBMCs, and biological activity in two cancer cell lines by MTT and comet assays, and pesticide efficiency. Via GC-MS, lidocaine was found to be the predominant compound in two of the studied species. To confirm the systematics, lidocaine was also found in lower amounts in 11 species. The MTT assay showed no toxicity in all six of the studied species. The comet assay showed the key result that the ethanol extracts of <i>Dd</i> and <i>Dg</i> violently broke DNA into pieces. Biological activity of these two species’ extracts showed toxicity on HepG2 and no effects on HCT-116. The water extracts of <i>Dd</i> and <i>Dg</i>, applied to <i>Brassica chinensis</i> showed high efficiency as a bioprotectant. In summary, lidocaine seems to be the predominant identifying compound of the genus <i>Dioscorea</i> in Thailand, which is useful in systematics. At least the two species, <i>Dd</i> and <i>Dg,</i> may be used for human hepatocyte cancer treatment and as an alternative pesticide for economically important vegetables. <i>Dioscorea</i> species containing lidocaine or extracted lidocaine have promise for natural product creation. |
| first_indexed | 2024-03-10T08:28:33Z |
| format | Article |
| id | doaj.art-9f0a2a4d30f5402da8bf706fd804dc7e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T08:28:33Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9f0a2a4d30f5402da8bf706fd804dc7e2023-11-22T09:17:59ZengMDPI AGPlants2223-77472021-07-01108155110.3390/plants10081551The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and ProductWarin Wonok0Arunrat Chaveerach1Pornnarong Siripiyasing2Runglawan Sudmoon3Tawatchai Tanee4Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Science and Technology, Rajabhat Mahasarakham University, Maha Sarakham 44000, ThailandFaculty of Law, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham 44150, ThailandThe six <i>Dioscorea</i> species, <i>D. brevipetiolata</i>, <i>D. bulbifera</i>, <i>D. depauperata</i> (<i>Dd</i>), <i>D. glabra</i> (<i>Dg</i>), <i>D. pyrifolia</i> and <i>D. hamiltonii</i> were analyzed for phytochemicals, toxicity in PBMCs, and biological activity in two cancer cell lines by MTT and comet assays, and pesticide efficiency. Via GC-MS, lidocaine was found to be the predominant compound in two of the studied species. To confirm the systematics, lidocaine was also found in lower amounts in 11 species. The MTT assay showed no toxicity in all six of the studied species. The comet assay showed the key result that the ethanol extracts of <i>Dd</i> and <i>Dg</i> violently broke DNA into pieces. Biological activity of these two species’ extracts showed toxicity on HepG2 and no effects on HCT-116. The water extracts of <i>Dd</i> and <i>Dg</i>, applied to <i>Brassica chinensis</i> showed high efficiency as a bioprotectant. In summary, lidocaine seems to be the predominant identifying compound of the genus <i>Dioscorea</i> in Thailand, which is useful in systematics. At least the two species, <i>Dd</i> and <i>Dg,</i> may be used for human hepatocyte cancer treatment and as an alternative pesticide for economically important vegetables. <i>Dioscorea</i> species containing lidocaine or extracted lidocaine have promise for natural product creation.https://www.mdpi.com/2223-7747/10/8/1551<i>Brassica chinensis</i><i>Dioscorea depauperata</i><i>Dioscorea glabra</i>HepG2HCT-116human hepatocyte cancer |
| spellingShingle | Warin Wonok Arunrat Chaveerach Pornnarong Siripiyasing Runglawan Sudmoon Tawatchai Tanee The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product Plants <i>Brassica chinensis</i> <i>Dioscorea depauperata</i> <i>Dioscorea glabra</i> HepG2 HCT-116 human hepatocyte cancer |
| title | The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product |
| title_full | The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product |
| title_fullStr | The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product |
| title_full_unstemmed | The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product |
| title_short | The Unique Substance, Lidocaine and Biological Activity of the <i>Dioscorea</i> Species for Potential Application as a Cancer Treatment, Natural Pesticide and Product |
| title_sort | unique substance lidocaine and biological activity of the i dioscorea i species for potential application as a cancer treatment natural pesticide and product |
| topic | <i>Brassica chinensis</i> <i>Dioscorea depauperata</i> <i>Dioscorea glabra</i> HepG2 HCT-116 human hepatocyte cancer |
| url | https://www.mdpi.com/2223-7747/10/8/1551 |
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