Pilot-scale enrichment of anammox biofilm using secondary effluent as source water

Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for...

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Main Authors: Runxian Tao, Xingcan Zheng, Xingfang Guo, Mai Li, Shifeng Shen, Min Yang, Yongli Sun, Fansong Wu
Format: Article
Language:English
Published: IWA Publishing 2021-02-01
Series:Water Science and Technology
Subjects:
Online Access:http://wst.iwaponline.com/content/83/4/894
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author Runxian Tao
Xingcan Zheng
Xingfang Guo
Mai Li
Shifeng Shen
Min Yang
Yongli Sun
Fansong Wu
author_facet Runxian Tao
Xingcan Zheng
Xingfang Guo
Mai Li
Shifeng Shen
Min Yang
Yongli Sun
Fansong Wu
author_sort Runxian Tao
collection DOAJ
description Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for enrichment of anammox biofilm by using secondary effluent as source water with no supplement of mineral medium and unstrict control of influent dissolved oxygen (DO). Anammox biofilm was successfully enriched in two pilot-scale reactors (XQ-cul and BT-cul) within 250 and 120 days, respectively. The specific anammox activity increased rapidly during the last 2 months in both reactors and achieved 2.54 g N2-N/(m2·d) in XQ-cul and 1.61 g N2-N/(m2·d) in BT-cul. Similar microbial diversity and community structure were obtained in the two reactors despite different secondary effluent being applied from two wastewater treatment plants. Anaerobic ammonium oxidizing bacteria genera abundance reached up to 37.4% and 43.1% in XQ-cul and BT-cul biofilm, respectively. Candidatus Brocadia and Ca. Kuenenia dominated the enriched biofilm. A negligible adverse effect of residual organics and influent DO was observed by using secondary effluent as source water. This anammox biofilm enrichment procedure could facilitate the inoculation and/or bio-augmentation of large-scale mainstream PN/A reactors.
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spelling doaj.art-9f0d1f35e78846b5b60dae31d705636a2022-12-21T19:59:58ZengIWA PublishingWater Science and Technology0273-12231996-97322021-02-0183489490510.2166/wst.2021.015015Pilot-scale enrichment of anammox biofilm using secondary effluent as source waterRunxian Tao0Xingcan Zheng1Xingfang Guo2Mai Li3Shifeng Shen4Min Yang5Yongli Sun6Fansong Wu7 North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for enrichment of anammox biofilm by using secondary effluent as source water with no supplement of mineral medium and unstrict control of influent dissolved oxygen (DO). Anammox biofilm was successfully enriched in two pilot-scale reactors (XQ-cul and BT-cul) within 250 and 120 days, respectively. The specific anammox activity increased rapidly during the last 2 months in both reactors and achieved 2.54 g N2-N/(m2·d) in XQ-cul and 1.61 g N2-N/(m2·d) in BT-cul. Similar microbial diversity and community structure were obtained in the two reactors despite different secondary effluent being applied from two wastewater treatment plants. Anaerobic ammonium oxidizing bacteria genera abundance reached up to 37.4% and 43.1% in XQ-cul and BT-cul biofilm, respectively. Candidatus Brocadia and Ca. Kuenenia dominated the enriched biofilm. A negligible adverse effect of residual organics and influent DO was observed by using secondary effluent as source water. This anammox biofilm enrichment procedure could facilitate the inoculation and/or bio-augmentation of large-scale mainstream PN/A reactors.http://wst.iwaponline.com/content/83/4/894anammoxbiofilmenrichment procedurelarge scalemicrobial communitysecondary effluent
spellingShingle Runxian Tao
Xingcan Zheng
Xingfang Guo
Mai Li
Shifeng Shen
Min Yang
Yongli Sun
Fansong Wu
Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
Water Science and Technology
anammox
biofilm
enrichment procedure
large scale
microbial community
secondary effluent
title Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
title_full Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
title_fullStr Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
title_full_unstemmed Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
title_short Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
title_sort pilot scale enrichment of anammox biofilm using secondary effluent as source water
topic anammox
biofilm
enrichment procedure
large scale
microbial community
secondary effluent
url http://wst.iwaponline.com/content/83/4/894
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