Pilot-scale enrichment of anammox biofilm using secondary effluent as source water
Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for...
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IWA Publishing
2021-02-01
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Online Access: | http://wst.iwaponline.com/content/83/4/894 |
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author | Runxian Tao Xingcan Zheng Xingfang Guo Mai Li Shifeng Shen Min Yang Yongli Sun Fansong Wu |
author_facet | Runxian Tao Xingcan Zheng Xingfang Guo Mai Li Shifeng Shen Min Yang Yongli Sun Fansong Wu |
author_sort | Runxian Tao |
collection | DOAJ |
description | Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for enrichment of anammox biofilm by using secondary effluent as source water with no supplement of mineral medium and unstrict control of influent dissolved oxygen (DO). Anammox biofilm was successfully enriched in two pilot-scale reactors (XQ-cul and BT-cul) within 250 and 120 days, respectively. The specific anammox activity increased rapidly during the last 2 months in both reactors and achieved 2.54 g N2-N/(m2·d) in XQ-cul and 1.61 g N2-N/(m2·d) in BT-cul. Similar microbial diversity and community structure were obtained in the two reactors despite different secondary effluent being applied from two wastewater treatment plants. Anaerobic ammonium oxidizing bacteria genera abundance reached up to 37.4% and 43.1% in XQ-cul and BT-cul biofilm, respectively. Candidatus Brocadia and Ca. Kuenenia dominated the enriched biofilm. A negligible adverse effect of residual organics and influent DO was observed by using secondary effluent as source water. This anammox biofilm enrichment procedure could facilitate the inoculation and/or bio-augmentation of large-scale mainstream PN/A reactors. |
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id | doaj.art-9f0d1f35e78846b5b60dae31d705636a |
institution | Directory Open Access Journal |
issn | 0273-1223 1996-9732 |
language | English |
last_indexed | 2024-12-20T00:30:04Z |
publishDate | 2021-02-01 |
publisher | IWA Publishing |
record_format | Article |
series | Water Science and Technology |
spelling | doaj.art-9f0d1f35e78846b5b60dae31d705636a2022-12-21T19:59:58ZengIWA PublishingWater Science and Technology0273-12231996-97322021-02-0183489490510.2166/wst.2021.015015Pilot-scale enrichment of anammox biofilm using secondary effluent as source waterRunxian Tao0Xingcan Zheng1Xingfang Guo2Mai Li3Shifeng Shen4Min Yang5Yongli Sun6Fansong Wu7 North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China North China Municipal Engineering Design & Research Institute Co., Tianjin 300074, China Enough biomass of anaerobic ammonium oxidation (anammox) bacteria is essential for maintaining a stable partial nitrification/anammox (PN/A) wastewater treatment system. Present enrichment procedures are mainly labor-intensive and inconvenient for up-scaling. A simplified procedure was developed for enrichment of anammox biofilm by using secondary effluent as source water with no supplement of mineral medium and unstrict control of influent dissolved oxygen (DO). Anammox biofilm was successfully enriched in two pilot-scale reactors (XQ-cul and BT-cul) within 250 and 120 days, respectively. The specific anammox activity increased rapidly during the last 2 months in both reactors and achieved 2.54 g N2-N/(m2·d) in XQ-cul and 1.61 g N2-N/(m2·d) in BT-cul. Similar microbial diversity and community structure were obtained in the two reactors despite different secondary effluent being applied from two wastewater treatment plants. Anaerobic ammonium oxidizing bacteria genera abundance reached up to 37.4% and 43.1% in XQ-cul and BT-cul biofilm, respectively. Candidatus Brocadia and Ca. Kuenenia dominated the enriched biofilm. A negligible adverse effect of residual organics and influent DO was observed by using secondary effluent as source water. This anammox biofilm enrichment procedure could facilitate the inoculation and/or bio-augmentation of large-scale mainstream PN/A reactors.http://wst.iwaponline.com/content/83/4/894anammoxbiofilmenrichment procedurelarge scalemicrobial communitysecondary effluent |
spellingShingle | Runxian Tao Xingcan Zheng Xingfang Guo Mai Li Shifeng Shen Min Yang Yongli Sun Fansong Wu Pilot-scale enrichment of anammox biofilm using secondary effluent as source water Water Science and Technology anammox biofilm enrichment procedure large scale microbial community secondary effluent |
title | Pilot-scale enrichment of anammox biofilm using secondary effluent as source water |
title_full | Pilot-scale enrichment of anammox biofilm using secondary effluent as source water |
title_fullStr | Pilot-scale enrichment of anammox biofilm using secondary effluent as source water |
title_full_unstemmed | Pilot-scale enrichment of anammox biofilm using secondary effluent as source water |
title_short | Pilot-scale enrichment of anammox biofilm using secondary effluent as source water |
title_sort | pilot scale enrichment of anammox biofilm using secondary effluent as source water |
topic | anammox biofilm enrichment procedure large scale microbial community secondary effluent |
url | http://wst.iwaponline.com/content/83/4/894 |
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