Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli.
Alpha-lipoic acid (LA) is an important enzyme cofactor widely used by organisms and is also a natural antioxidant for the treatment of pathologies driven by low levels of endogenous antioxidants. In order to establish a safer and more efficient process for LA production, we developed a new biologica...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5222372?pdf=render |
| _version_ | 1818608044160843776 |
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| author | Yirong Sun Wenbin Zhang Jincheng Ma Hongshen Pang Haihong Wang |
| author_facet | Yirong Sun Wenbin Zhang Jincheng Ma Hongshen Pang Haihong Wang |
| author_sort | Yirong Sun |
| collection | DOAJ |
| description | Alpha-lipoic acid (LA) is an important enzyme cofactor widely used by organisms and is also a natural antioxidant for the treatment of pathologies driven by low levels of endogenous antioxidants. In order to establish a safer and more efficient process for LA production, we developed a new biological method for LA synthesis based on the emerging knowledge of lipoic acid biosynthesis. We first cloned the lipD gene, which encodes the lipoyl domain of the E2 subunit of pyruvate dehydrogenase, allowing high levels of LipD production. Plasmids containing genes for the biosynthesis of LA were subsequently constructed utilizing various vectors and promotors to produce high levels of LA. These plasmids were transformed into the Escherichia coli strain BL21. Octanoic acid (OA) was used as the substrate for LA synthesis. One transformant, YS61, which carried lipD, lplA, and lipA, produced LA at levels over 200-fold greater than the wild-type strain, showing that LA could be produced efficiently in E. coli using genetic engineering methods. |
| first_indexed | 2024-12-16T14:36:23Z |
| format | Article |
| id | doaj.art-9f0f6d3a6b904d5ead6b0415aedd1689 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T14:36:23Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9f0f6d3a6b904d5ead6b0415aedd16892022-12-21T22:28:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e016936910.1371/journal.pone.0169369Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli.Yirong SunWenbin ZhangJincheng MaHongshen PangHaihong WangAlpha-lipoic acid (LA) is an important enzyme cofactor widely used by organisms and is also a natural antioxidant for the treatment of pathologies driven by low levels of endogenous antioxidants. In order to establish a safer and more efficient process for LA production, we developed a new biological method for LA synthesis based on the emerging knowledge of lipoic acid biosynthesis. We first cloned the lipD gene, which encodes the lipoyl domain of the E2 subunit of pyruvate dehydrogenase, allowing high levels of LipD production. Plasmids containing genes for the biosynthesis of LA were subsequently constructed utilizing various vectors and promotors to produce high levels of LA. These plasmids were transformed into the Escherichia coli strain BL21. Octanoic acid (OA) was used as the substrate for LA synthesis. One transformant, YS61, which carried lipD, lplA, and lipA, produced LA at levels over 200-fold greater than the wild-type strain, showing that LA could be produced efficiently in E. coli using genetic engineering methods.http://europepmc.org/articles/PMC5222372?pdf=render |
| spellingShingle | Yirong Sun Wenbin Zhang Jincheng Ma Hongshen Pang Haihong Wang Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli. PLoS ONE |
| title | Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli. |
| title_full | Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli. |
| title_fullStr | Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli. |
| title_full_unstemmed | Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli. |
| title_short | Overproduction of α-Lipoic Acid by Gene Manipulated Escherichia coli. |
| title_sort | overproduction of α lipoic acid by gene manipulated escherichia coli |
| url | http://europepmc.org/articles/PMC5222372?pdf=render |
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