The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds

Sugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than...

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Main Authors: Antov Mirjana G., Fišteš Aleksandar Z.
Format: Article
Language:English
Published: Faculty of Technology, Novi Sad 2018-01-01
Series:Acta Periodica Technologica
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/1450-7188/2018/1450-71881849001A.pdf
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author Antov Mirjana G.
Fišteš Aleksandar Z.
author_facet Antov Mirjana G.
Fišteš Aleksandar Z.
author_sort Antov Mirjana G.
collection DOAJ
description Sugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than in untreated material, resulting in more than two times higher release of reducing sugars. In the investigated range of solids load, supplementation of fungal cellulases cocktail by beta-glucosidase increased production of reducing sugars from substrates, while 0.25 U/g was sufficient to achieve the highest effect under applied conditions.[ Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31002]
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spelling doaj.art-9f13084cbbe44ea9bae37f8a723595222022-12-21T19:10:15ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882406-095X2018-01-012018491910.2298/APT1849001A1450-71881849001AThe effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shredsAntov Mirjana G.0Fišteš Aleksandar Z.1Faculty of Technology, Novi SadFaculty of Technology, Novi SadSugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than in untreated material, resulting in more than two times higher release of reducing sugars. In the investigated range of solids load, supplementation of fungal cellulases cocktail by beta-glucosidase increased production of reducing sugars from substrates, while 0.25 U/g was sufficient to achieve the highest effect under applied conditions.[ Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31002]http://www.doiserbia.nb.rs/img/doi/1450-7188/2018/1450-71881849001A.pdfenzymatic hydrolysisbeta-glucosidasecellulosehydrothermal pre-treatmentsugar beet shreds
spellingShingle Antov Mirjana G.
Fišteš Aleksandar Z.
The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
Acta Periodica Technologica
enzymatic hydrolysis
beta-glucosidase
cellulose
hydrothermal pre-treatment
sugar beet shreds
title The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
title_full The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
title_fullStr The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
title_full_unstemmed The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
title_short The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
title_sort effect of beta glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
topic enzymatic hydrolysis
beta-glucosidase
cellulose
hydrothermal pre-treatment
sugar beet shreds
url http://www.doiserbia.nb.rs/img/doi/1450-7188/2018/1450-71881849001A.pdf
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