The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds
Sugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than...
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Format: | Article |
Language: | English |
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Faculty of Technology, Novi Sad
2018-01-01
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Series: | Acta Periodica Technologica |
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Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2018/1450-71881849001A.pdf |
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author | Antov Mirjana G. Fišteš Aleksandar Z. |
author_facet | Antov Mirjana G. Fišteš Aleksandar Z. |
author_sort | Antov Mirjana G. |
collection | DOAJ |
description | Sugar beet shreds were pretreated by hydrothermal procedure to investigate
the effect of beta-glucosidase supplementation at different substrate
loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally
pretreated substrate was more efficiently hydrolyzed by enzymes than in
untreated material, resulting in more than two times higher release of
reducing sugars. In the investigated range of solids load, supplementation
of fungal cellulases cocktail by beta-glucosidase increased production of
reducing sugars from substrates, while 0.25 U/g was sufficient to achieve
the highest effect under applied conditions.[ Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31002] |
first_indexed | 2024-12-21T08:28:50Z |
format | Article |
id | doaj.art-9f13084cbbe44ea9bae37f8a72359522 |
institution | Directory Open Access Journal |
issn | 1450-7188 2406-095X |
language | English |
last_indexed | 2024-12-21T08:28:50Z |
publishDate | 2018-01-01 |
publisher | Faculty of Technology, Novi Sad |
record_format | Article |
series | Acta Periodica Technologica |
spelling | doaj.art-9f13084cbbe44ea9bae37f8a723595222022-12-21T19:10:15ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882406-095X2018-01-012018491910.2298/APT1849001A1450-71881849001AThe effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shredsAntov Mirjana G.0Fišteš Aleksandar Z.1Faculty of Technology, Novi SadFaculty of Technology, Novi SadSugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than in untreated material, resulting in more than two times higher release of reducing sugars. In the investigated range of solids load, supplementation of fungal cellulases cocktail by beta-glucosidase increased production of reducing sugars from substrates, while 0.25 U/g was sufficient to achieve the highest effect under applied conditions.[ Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31002]http://www.doiserbia.nb.rs/img/doi/1450-7188/2018/1450-71881849001A.pdfenzymatic hydrolysisbeta-glucosidasecellulosehydrothermal pre-treatmentsugar beet shreds |
spellingShingle | Antov Mirjana G. Fišteš Aleksandar Z. The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds Acta Periodica Technologica enzymatic hydrolysis beta-glucosidase cellulose hydrothermal pre-treatment sugar beet shreds |
title | The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds |
title_full | The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds |
title_fullStr | The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds |
title_full_unstemmed | The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds |
title_short | The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds |
title_sort | effect of beta glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds |
topic | enzymatic hydrolysis beta-glucosidase cellulose hydrothermal pre-treatment sugar beet shreds |
url | http://www.doiserbia.nb.rs/img/doi/1450-7188/2018/1450-71881849001A.pdf |
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