Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs)
Abstract Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-04-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01221-9 |
| _version_ | 1818451726337835008 |
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| author | Cheng Yang Wangfeng Shao Lingling Wei Lingxiao Chen Aihua Zhu Zhiming Pan |
| author_facet | Cheng Yang Wangfeng Shao Lingling Wei Lingxiao Chen Aihua Zhu Zhiming Pan |
| author_sort | Cheng Yang |
| collection | DOAJ |
| description | Abstract Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis. |
| first_indexed | 2024-12-14T21:11:47Z |
| format | Article |
| id | doaj.art-9f1349ed64cb4469a6b8389189590043 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-14T21:11:47Z |
| publishDate | 2021-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-9f1349ed64cb4469a6b83891895900432022-12-21T22:47:13ZengSpringerOpenAMB Express2191-08552021-04-011111810.1186/s13568-021-01221-9Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs)Cheng Yang0Wangfeng Shao1Lingling Wei2Lingxiao Chen3Aihua Zhu4Zhiming Pan5College of Health Sciences, Jiangsu Normal UniversityCollege of Health Sciences, Jiangsu Normal UniversityCollege of Health Sciences, Jiangsu Normal UniversityCollege of Health Sciences, Jiangsu Normal UniversityCollege of Health Sciences, Jiangsu Normal UniversityJiangsu Co-Innovationnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityAbstract Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis.https://doi.org/10.1186/s13568-021-01221-9SalmonellaPetMultilocus sequence typingClustered regularly interspaced short palindromic repeatsMolecular subtyping |
| spellingShingle | Cheng Yang Wangfeng Shao Lingling Wei Lingxiao Chen Aihua Zhu Zhiming Pan Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) AMB Express Salmonella Pet Multilocus sequence typing Clustered regularly interspaced short palindromic repeats Molecular subtyping |
| title | Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) |
| title_full | Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) |
| title_fullStr | Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) |
| title_full_unstemmed | Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) |
| title_short | Subtyping Salmonella isolated from pet dogs with multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) |
| title_sort | subtyping salmonella isolated from pet dogs with multilocus sequence typing mlst and clustered regularly interspaced short palindromic repeats crisprs |
| topic | Salmonella Pet Multilocus sequence typing Clustered regularly interspaced short palindromic repeats Molecular subtyping |
| url | https://doi.org/10.1186/s13568-021-01221-9 |
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