Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications
Abstract The present paper evaluates the photocatalytic degradation (PCD) performance of the biofabricated hematite nanoparticles (α-HNPs) for the degradation approach of the Cefotaxime (Cfm). The optimum pH of the solution to achieve the best PCD was found to be 10.5. The kinetics study for the PCD...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14922-3 |
| _version_ | 1818198687369658368 |
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| author | Mostafa F. Al-Hakkani Gamal A. Gouda Sedky H. A. Hassan Mohammed S. Saddik Mohamed A. El-Mokhtar Maggie A. Ibrahim Mahmoud M. A. Mohamed Adham M. Nagiub |
| author_facet | Mostafa F. Al-Hakkani Gamal A. Gouda Sedky H. A. Hassan Mohammed S. Saddik Mohamed A. El-Mokhtar Maggie A. Ibrahim Mahmoud M. A. Mohamed Adham M. Nagiub |
| author_sort | Mostafa F. Al-Hakkani |
| collection | DOAJ |
| description | Abstract The present paper evaluates the photocatalytic degradation (PCD) performance of the biofabricated hematite nanoparticles (α-HNPs) for the degradation approach of the Cefotaxime (Cfm). The optimum pH of the solution to achieve the best PCD was found to be 10.5. The kinetics study for the PCD of the Cfm via α-HNPs has been investigated and the reaction was found to be fellow pseudo-first-order at R2 = 0.992. The mass loading impact of α-HNPs was investigated and estimated for the maximum degradation of Cfm 0.4 mg/mL. UV–Vis confirmed that α-HNPs had a direct transition bandgap at 3.78 eV at a maximum absorption wavelength of 362 nm with suspension stability for 7 days. The probable mechanism of the Cfm PCD via α-HNPs and the degradation pathway was conducted. The validation of the suspension stability of the α-HNPs (−68.6 ± 11.8 mV) was determined using the zeta potential investigation test. XRD investigation was conducted after Cfm PCD showing an average crystallite size of 27.0 nm. XRD, TEM, SEM, EDX, and FT-IR analyses have been conducted for the α-HNPs before and after Cfm PCD confirming the high efficiency for the reusability of the current biocatalyst α-HNPs for further use. TEM results of the particle sizes of α-HNPs were found at 19.2 ± 4.4 and 20.6 ± 7.4 nm respectively before and after Cfm PCD. The efficiency of the Cfm PCD was found to be 99.1% after 6 h. High potent as an antibacterial agent of α-HNPs was investigated either α-HNPs alone or after its PCD activity against Cfm. The antibacterial activity revealed high sensitivity, especially toward Gram-positive species indicating its promising ability against pathogenic issues. Interestingly, Cfm@α-HNPs showed superior anti-proliferative activity as tested by MTT assay and were able to induce apoptosis in MCF7 and HepG2 cell lines using the flow cytometry technique at 20.7% and 17% respectively. Also, The IC50 of hydrogen peroxide scavenging was estimated and it was manifested that 635.8 and 665.6 μg/mL of α-HNPs before and after the PCD process of Cfm respectively. |
| first_indexed | 2024-12-12T02:09:50Z |
| format | Article |
| id | doaj.art-9f135ffb51224d5e8cee2c7c3301cb3d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T02:09:50Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9f135ffb51224d5e8cee2c7c3301cb3d2022-12-22T00:41:56ZengNature PortfolioScientific Reports2045-23222022-07-0112112010.1038/s41598-022-14922-3Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applicationsMostafa F. Al-Hakkani0Gamal A. Gouda1Sedky H. A. Hassan2Mohammed S. Saddik3Mohamed A. El-Mokhtar4Maggie A. Ibrahim5Mahmoud M. A. Mohamed6Adham M. Nagiub7Department of Chemistry, Faculty of Science, Al-Azhar University, Assiut BranchDepartment of Chemistry, Faculty of Science, Al-Azhar University, Assiut BranchDepartment of Biology, College of Science, Sultan Qaboos UniversityDepartment of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut UniversityDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut UniversityDepartment of Chemistry, Faculty of Science, New Valley UniversityDepartment of Chemistry, Faculty of Science, Al-Azhar University, Assiut BranchAbstract The present paper evaluates the photocatalytic degradation (PCD) performance of the biofabricated hematite nanoparticles (α-HNPs) for the degradation approach of the Cefotaxime (Cfm). The optimum pH of the solution to achieve the best PCD was found to be 10.5. The kinetics study for the PCD of the Cfm via α-HNPs has been investigated and the reaction was found to be fellow pseudo-first-order at R2 = 0.992. The mass loading impact of α-HNPs was investigated and estimated for the maximum degradation of Cfm 0.4 mg/mL. UV–Vis confirmed that α-HNPs had a direct transition bandgap at 3.78 eV at a maximum absorption wavelength of 362 nm with suspension stability for 7 days. The probable mechanism of the Cfm PCD via α-HNPs and the degradation pathway was conducted. The validation of the suspension stability of the α-HNPs (−68.6 ± 11.8 mV) was determined using the zeta potential investigation test. XRD investigation was conducted after Cfm PCD showing an average crystallite size of 27.0 nm. XRD, TEM, SEM, EDX, and FT-IR analyses have been conducted for the α-HNPs before and after Cfm PCD confirming the high efficiency for the reusability of the current biocatalyst α-HNPs for further use. TEM results of the particle sizes of α-HNPs were found at 19.2 ± 4.4 and 20.6 ± 7.4 nm respectively before and after Cfm PCD. The efficiency of the Cfm PCD was found to be 99.1% after 6 h. High potent as an antibacterial agent of α-HNPs was investigated either α-HNPs alone or after its PCD activity against Cfm. The antibacterial activity revealed high sensitivity, especially toward Gram-positive species indicating its promising ability against pathogenic issues. Interestingly, Cfm@α-HNPs showed superior anti-proliferative activity as tested by MTT assay and were able to induce apoptosis in MCF7 and HepG2 cell lines using the flow cytometry technique at 20.7% and 17% respectively. Also, The IC50 of hydrogen peroxide scavenging was estimated and it was manifested that 635.8 and 665.6 μg/mL of α-HNPs before and after the PCD process of Cfm respectively.https://doi.org/10.1038/s41598-022-14922-3 |
| spellingShingle | Mostafa F. Al-Hakkani Gamal A. Gouda Sedky H. A. Hassan Mohammed S. Saddik Mohamed A. El-Mokhtar Maggie A. Ibrahim Mahmoud M. A. Mohamed Adham M. Nagiub Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications Scientific Reports |
| title | Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications |
| title_full | Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications |
| title_fullStr | Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications |
| title_full_unstemmed | Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications |
| title_short | Cefotaxime removal enhancement via bio-nanophotocatalyst α-Fe2O3 using photocatalytic degradation technique and its echo-biomedical applications |
| title_sort | cefotaxime removal enhancement via bio nanophotocatalyst α fe2o3 using photocatalytic degradation technique and its echo biomedical applications |
| url | https://doi.org/10.1038/s41598-022-14922-3 |
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