A quick, cheap, and reliable protocol for immunofluorescence of pluripotent and differentiating mouse embryonic stem cells in 2D and 3D colonies

A quick, cheap, and reliable protocol for immunofluorescence of pluripotent and differentiating mouse embryonic stem cells in 2D and 3D colonies

Summary: Immunofluorescent labeling is a widely used method to visualize endogenous proteins. It can be expensive and difficult to stain mouse embryonic stem cells (mESCs) because they require expensive growth media, prefer specific substrates, grow in 3D, and have loose cell-substrate adhesion. Her...

Mô tả đầy đủ

Chi tiết về thư mục
Những tác giả chính:	Snježana Kodba, Agathe Chaigne
Định dạng:	Bài viết
Ngôn ngữ:	English
Được phát hành:	Elsevier 2023-03-01
Loạt:	STAR Protocols
Những chủ đề:	Cell Biology Developmental biology Stem Cells
Truy cập trực tuyến:	http://www.sciencedirect.com/science/article/pii/S2666166722008802

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