Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus
Erianthus arundinaceus has a great potential in bioenergy production and green-house gas mitigation due to its biological characteristic of huge biomass yield and stress tolerance. Our earlier transcriptome studies on a set of sugarcane genotypes differing for lignin content, showed that Cinnamyl Al...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Society for Sugarcane Research and Development
2021-06-01
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| Series: | Journal of Sugarcane Research |
| Subjects: | |
| Online Access: | http://epubs.icar.org.in/ejournal/index.php/JSR/article/view/106418 |
| _version_ | 1819104120758337536 |
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| author | Lakshmi Kasirajan Rabisha Valiyaparambath |
| author_facet | Lakshmi Kasirajan Rabisha Valiyaparambath |
| author_sort | Lakshmi Kasirajan |
| collection | DOAJ |
| description | Erianthus arundinaceus has a great potential in bioenergy production and green-house gas mitigation due to its biological characteristic of huge biomass yield and stress tolerance. Our earlier transcriptome studies on a set of sugarcane genotypes differing for lignin content, showed that Cinnamyl Alcohol Dehydrogenase (CAD), was differentially expressed and of plays a major role in genetic regulation of lignin production, stem development and defense. This study aims to characterize the CAD gene and its expression in BL 21 E. Coli strain. In this context, understanding the regulatory functions of CAD gene would be more useful for achieving reduced lignin content and to have a cost-effective biomass conversion in sugarcane. Using bioinformatic tools like ExPasy and Prosite, the sugarcane CAD was found to belong to the Class I with affinity towards coniferyl aldehyde, sinapyl aldehyde with a predicted molecular weight of ~46kDa. |
| first_indexed | 2024-12-22T02:01:18Z |
| format | Article |
| id | doaj.art-9f168152dc464dce82e81112149251ca |
| institution | Directory Open Access Journal |
| issn | 2249-927X 2582-4767 |
| language | English |
| last_indexed | 2024-12-22T02:01:18Z |
| publishDate | 2021-06-01 |
| publisher | Society for Sugarcane Research and Development |
| record_format | Article |
| series | Journal of Sugarcane Research |
| spelling | doaj.art-9f168152dc464dce82e81112149251ca2022-12-21T18:42:39ZengSociety for Sugarcane Research and DevelopmentJournal of Sugarcane Research2249-927X2582-47672021-06-0110210.37580/JSR.2020.2.10.152-15746354Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceusLakshmi Kasirajan0Rabisha Valiyaparambath1ICAR Sugarcane Breeding Institute, CBEICAR Sugarcane Breeding Institute, CBEErianthus arundinaceus has a great potential in bioenergy production and green-house gas mitigation due to its biological characteristic of huge biomass yield and stress tolerance. Our earlier transcriptome studies on a set of sugarcane genotypes differing for lignin content, showed that Cinnamyl Alcohol Dehydrogenase (CAD), was differentially expressed and of plays a major role in genetic regulation of lignin production, stem development and defense. This study aims to characterize the CAD gene and its expression in BL 21 E. Coli strain. In this context, understanding the regulatory functions of CAD gene would be more useful for achieving reduced lignin content and to have a cost-effective biomass conversion in sugarcane. Using bioinformatic tools like ExPasy and Prosite, the sugarcane CAD was found to belong to the Class I with affinity towards coniferyl aldehyde, sinapyl aldehyde with a predicted molecular weight of ~46kDa.http://epubs.icar.org.in/ejournal/index.php/JSR/article/view/106418monolignolslignificationproteinsecondary cell wall |
| spellingShingle | Lakshmi Kasirajan Rabisha Valiyaparambath Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus Journal of Sugarcane Research monolignols lignification protein secondary cell wall |
| title | Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus |
| title_full | Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus |
| title_fullStr | Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus |
| title_full_unstemmed | Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus |
| title_short | Expression analysis of Cinnamyl Alcohol Dehydrogenase (CAD) involved in lignin biosynthesis of Erianthus arundinaceus |
| title_sort | expression analysis of cinnamyl alcohol dehydrogenase cad involved in lignin biosynthesis of erianthus arundinaceus |
| topic | monolignols lignification protein secondary cell wall |
| url | http://epubs.icar.org.in/ejournal/index.php/JSR/article/view/106418 |
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