Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran
Background: The incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and −938C>A single nucleotide polymorphism (S...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Journal of Research in Medical Sciences |
| Subjects: | |
| Online Access: | http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2017;volume=22;issue=1;spage=21;epage=21;aulast=Moghaddam |
| _version_ | 1811258990541144064 |
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| author | Sepideh Esfahani Moghaddam Ali Barzegar Novin Nikbakhsh |
| author_facet | Sepideh Esfahani Moghaddam Ali Barzegar Novin Nikbakhsh |
| author_sort | Sepideh Esfahani Moghaddam |
| collection | DOAJ |
| description | Background: The incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and −938C>A single nucleotide polymorphism (SNP) of BCL2 gene promoter has been demonstrated to influence breast cancer susceptibility. In this research, we study the effect of −938C>A allelic variants on breast cancer risk in Mazandaran province at the North of Iran. Materials and Methods: This analysis performed on 120 breast cancer patients who underwent surgery in some referenced hospitals at Mazandaran province along with 130 healthy individuals as a control. DNA extracted from peripheral blood samples was applied in polymerase chain reaction-single-strand conformation polymorphism analysis to determine −938C>A genotype. The association of the −938C>A genotype and breast cancer risk as well as clinicopathological characters were analyzed by logistic regression method. Results: Results showed that genotype frequency of AA, AC, and CC genotypes was 10%, 62%, and 28% for case and 28%, 50%, and 22% in control group, respectively. In the logistic regression model, BCL2 − 938C/A variant genotype AA was associated with a decreased risk of breast cancer (P = 0.041) by 0.31-fold (odds ratio = 0.31, confidence interval = 0.091–0.909) compared to CC genotype. However, no significant association found between −938C>A genotype and clinicopathological characters. Conclusion: The study showed that AA genotype of BCL2 gene (−938C>A) is associated with decreased susceptibility to breast cancer. Hence, investigating the −938C>A SNP of BCL2 gene promoter could be an appropriate molecular marker to determine individual sensitivity to breast cancer. |
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| format | Article |
| id | doaj.art-9f16ac20c03343a08b0a09e6af097bd9 |
| institution | Directory Open Access Journal |
| issn | 1735-1995 1735-7136 |
| language | English |
| last_indexed | 2024-04-12T18:23:48Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Research in Medical Sciences |
| spelling | doaj.art-9f16ac20c03343a08b0a09e6af097bd92022-12-22T03:21:19ZengWolters Kluwer Medknow PublicationsJournal of Research in Medical Sciences1735-19951735-71362017-01-01221212110.4103/1735-1995.200269Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern IranSepideh Esfahani MoghaddamAli BarzegarNovin NikbakhshBackground: The incidence rate of breast cancer has been dramatically increasing since the last decade in Iran, and it is now one of the most common female malignant tumors. B-cell lymphoma 2 (BCL2) family is the most important regulator of apoptosis, and −938C>A single nucleotide polymorphism (SNP) of BCL2 gene promoter has been demonstrated to influence breast cancer susceptibility. In this research, we study the effect of −938C>A allelic variants on breast cancer risk in Mazandaran province at the North of Iran. Materials and Methods: This analysis performed on 120 breast cancer patients who underwent surgery in some referenced hospitals at Mazandaran province along with 130 healthy individuals as a control. DNA extracted from peripheral blood samples was applied in polymerase chain reaction-single-strand conformation polymorphism analysis to determine −938C>A genotype. The association of the −938C>A genotype and breast cancer risk as well as clinicopathological characters were analyzed by logistic regression method. Results: Results showed that genotype frequency of AA, AC, and CC genotypes was 10%, 62%, and 28% for case and 28%, 50%, and 22% in control group, respectively. In the logistic regression model, BCL2 − 938C/A variant genotype AA was associated with a decreased risk of breast cancer (P = 0.041) by 0.31-fold (odds ratio = 0.31, confidence interval = 0.091–0.909) compared to CC genotype. However, no significant association found between −938C>A genotype and clinicopathological characters. Conclusion: The study showed that AA genotype of BCL2 gene (−938C>A) is associated with decreased susceptibility to breast cancer. Hence, investigating the −938C>A SNP of BCL2 gene promoter could be an appropriate molecular marker to determine individual sensitivity to breast cancer.http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2017;volume=22;issue=1;spage=21;epage=21;aulast=Moghaddam−938C>AB-cell lymphoma 2breast cancerpolymerase chain reaction-single-strand conformation polymorphismpolymorphism |
| spellingShingle | Sepideh Esfahani Moghaddam Ali Barzegar Novin Nikbakhsh Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran Journal of Research in Medical Sciences −938C>A B-cell lymphoma 2 breast cancer polymerase chain reaction-single-strand conformation polymorphism polymorphism |
| title | Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran |
| title_full | Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran |
| title_fullStr | Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran |
| title_full_unstemmed | Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran |
| title_short | Study of the regulatory promoter polymorphism (−938C>A) of B-cell lymphoma 2 gene in breast cancer patients of Mazandaran province in Northern Iran |
| title_sort | study of the regulatory promoter polymorphism 938c a of b cell lymphoma 2 gene in breast cancer patients of mazandaran province in northern iran |
| topic | −938C>A B-cell lymphoma 2 breast cancer polymerase chain reaction-single-strand conformation polymorphism polymorphism |
| url | http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2017;volume=22;issue=1;spage=21;epage=21;aulast=Moghaddam |
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