Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia
Abstract Background Eye infection is a public health problem in developing countries including Ethiopia. Bacteria are major causative agents of eye infections that can lead to loss of vision. The objective of this study was to determine bacterial etiology of ocular and periocular infections, antimic...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-03-01
|
| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12886-020-01398-w |
| _version_ | 1818161780717780992 |
|---|---|
| author | Ahmed Adem Mohammed Musa Mohammed Ali Mengistu Hailemariam Zenebe |
| author_facet | Ahmed Adem Mohammed Musa Mohammed Ali Mengistu Hailemariam Zenebe |
| author_sort | Ahmed Adem Mohammed |
| collection | DOAJ |
| description | Abstract Background Eye infection is a public health problem in developing countries including Ethiopia. Bacteria are major causative agents of eye infections that can lead to loss of vision. The objective of this study was to determine bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients who visited the eye unit of Shashamane Comprehensive Specialized Hospital (SCSH). Method A hospital-based cross-sectional study was conducted at SCSH from September 1, 2018, to March 30, 2019. Specimens from the ocular and periocular areas were collected from a total of 332 patients who visited the eye unit. Specimens were inoculated on blood agar, chocolate agar, MacConkey agar, and mannitol salt agar. Isolated bacteria were identified by a series of biochemical tests using the standard bacteriological method. Antimicrobial susceptibility test was performed according to the Clinical and Laboratory Standard Institute by disk diffusion method. Factors that could be associated with ocular and periocular infection were collected by using structured questionnaire. Data analysis was done using SPSS version 22.0 software package. A P value less than 0.05 was considered statistically significant. Result Out of the total 332 study participants with ocular and periocular infections, 198(60%) were culture positive. The proportion of Gram-positive and Gram-negative bacteria were 135(68.2%) and 63(31.8%) respectively. Among Gram-positive bacteria, Staphylococcus aureus were predominant. Among Gram-negative bacteria, Escherichia coli were predominant. Most S. aureus were resistant to penicillin. Conclusion Majority of ocular and periocular infections in this study were caused by bacteria; Gram-positive bacteria were responsible for most cases. |
| first_indexed | 2024-12-11T16:23:13Z |
| format | Article |
| id | doaj.art-9f17281ea52a432d9ff3516d986952ff |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-11T16:23:13Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-9f17281ea52a432d9ff3516d986952ff2022-12-22T00:58:47ZengBMCBMC Ophthalmology1471-24152020-03-012011810.1186/s12886-020-01398-wBacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, EthiopiaAhmed Adem Mohammed0Musa Mohammed Ali1Mengistu Hailemariam Zenebe2Shashemene Comprehensive Specialized HospitalHawassa University College of Medicine and Health Sciences department of Medical laboratoryHawassa University College of Medicine and Health Sciences department of Medical laboratoryAbstract Background Eye infection is a public health problem in developing countries including Ethiopia. Bacteria are major causative agents of eye infections that can lead to loss of vision. The objective of this study was to determine bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients who visited the eye unit of Shashamane Comprehensive Specialized Hospital (SCSH). Method A hospital-based cross-sectional study was conducted at SCSH from September 1, 2018, to March 30, 2019. Specimens from the ocular and periocular areas were collected from a total of 332 patients who visited the eye unit. Specimens were inoculated on blood agar, chocolate agar, MacConkey agar, and mannitol salt agar. Isolated bacteria were identified by a series of biochemical tests using the standard bacteriological method. Antimicrobial susceptibility test was performed according to the Clinical and Laboratory Standard Institute by disk diffusion method. Factors that could be associated with ocular and periocular infection were collected by using structured questionnaire. Data analysis was done using SPSS version 22.0 software package. A P value less than 0.05 was considered statistically significant. Result Out of the total 332 study participants with ocular and periocular infections, 198(60%) were culture positive. The proportion of Gram-positive and Gram-negative bacteria were 135(68.2%) and 63(31.8%) respectively. Among Gram-positive bacteria, Staphylococcus aureus were predominant. Among Gram-negative bacteria, Escherichia coli were predominant. Most S. aureus were resistant to penicillin. Conclusion Majority of ocular and periocular infections in this study were caused by bacteria; Gram-positive bacteria were responsible for most cases.http://link.springer.com/article/10.1186/s12886-020-01398-wExternal eyeInfectionBacteriaRisk factorsAntimicrobial susceptibility pattern |
| spellingShingle | Ahmed Adem Mohammed Musa Mohammed Ali Mengistu Hailemariam Zenebe Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia BMC Ophthalmology External eye Infection Bacteria Risk factors Antimicrobial susceptibility pattern |
| title | Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia |
| title_full | Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia |
| title_fullStr | Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia |
| title_full_unstemmed | Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia |
| title_short | Bacterial etiology of ocular and periocular infections, antimicrobial susceptibility profile and associated factors among patients attending eye unit of Shashemene comprehensive specialized hospital, Shashemene, Ethiopia |
| title_sort | bacterial etiology of ocular and periocular infections antimicrobial susceptibility profile and associated factors among patients attending eye unit of shashemene comprehensive specialized hospital shashemene ethiopia |
| topic | External eye Infection Bacteria Risk factors Antimicrobial susceptibility pattern |
| url | http://link.springer.com/article/10.1186/s12886-020-01398-w |
| work_keys_str_mv | AT ahmedademmohammed bacterialetiologyofocularandperiocularinfectionsantimicrobialsusceptibilityprofileandassociatedfactorsamongpatientsattendingeyeunitofshashemenecomprehensivespecializedhospitalshashemeneethiopia AT musamohammedali bacterialetiologyofocularandperiocularinfectionsantimicrobialsusceptibilityprofileandassociatedfactorsamongpatientsattendingeyeunitofshashemenecomprehensivespecializedhospitalshashemeneethiopia AT mengistuhailemariamzenebe bacterialetiologyofocularandperiocularinfectionsantimicrobialsusceptibilityprofileandassociatedfactorsamongpatientsattendingeyeunitofshashemenecomprehensivespecializedhospitalshashemeneethiopia |