Increase of nitrosative stress in patients with eosinophilic pneumonia
<p>Abstract</p> <p>Background</p> <p>Exhaled nitric oxide (NO) production is increased in asthma and reflects the degree of airway inflammation. The alveolar NO concentration (Calv) in interstitial pneumonia is reported to be increased. However, it remains unknown wheth...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-06-01
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| Series: | Respiratory Research |
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| Online Access: | http://respiratory-research.com/content/12/1/81 |
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| author | Akamatsu Keiichiro Minakata Yoshiaki Yanagisawa Satoru Hirano Tsunahiko Koarai Akira Ichikawa Tomohiro Matsunaga Kazuto Sugiura Hisatoshi Furukawa Kanako Kanda Masae Nishigai Manabu Ichinose Masakazu |
| author_facet | Akamatsu Keiichiro Minakata Yoshiaki Yanagisawa Satoru Hirano Tsunahiko Koarai Akira Ichikawa Tomohiro Matsunaga Kazuto Sugiura Hisatoshi Furukawa Kanako Kanda Masae Nishigai Manabu Ichinose Masakazu |
| author_sort | Akamatsu Keiichiro |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Exhaled nitric oxide (NO) production is increased in asthma and reflects the degree of airway inflammation. The alveolar NO concentration (Calv) in interstitial pneumonia is reported to be increased. However, it remains unknown whether NO production is increased and nitrosative stress occurs in eosinophilic pneumonia (EP). We hypothesized that nitrosative stress markers including Calv, inducible type of NO synthase (iNOS), and 3-nitrotyrosine (3-NT), are upregulated in EP.</p> <p>Methods</p> <p>Exhaled NO including fractional exhaled NO (FE<sub>NO</sub>) and Calv was measured in ten healthy subjects, 13 patients with idiopathic pulmonary fibrosis (IPF), and 13 patients with EP. iNOS expression and 3-NT formation were assessed by immunocytochemistory in BALf cells. The exhaled NO, lung function, and systemic inflammatory markers of the EP patients were investigated after corticosteroid treatment for 4 weeks.</p> <p>Results</p> <p>The Calv levels in the EP group (14.4 ± 2.0 ppb) were significantly higher than those in the healthy subjects (5.1 ± 0.6 ppb, p < 0.01) and the IPF groups (6.3 ± 0.6 ppb, p < 0.01) as well as the FE<sub>NO </sub>and the corrected Calv levels (all p < 0.01). More iNOS and 3-NT positive cells were observed in the EP group compared to the healthy subject and IPF patient. The Calv levels had significant positive correlations with both iNOS (r = 0.858, p < 0.05) and 3-NT positive cells (r = 0.924, p < 0.01). Corticosteroid treatment significantly reduced both the FE<sub>NO </sub>(p < 0.05) and the Calv levels (p < 0.01). The magnitude of reduction in the Calv levels had a significant positive correlation with the peripheral blood eosinophil counts (r = 0.802, p < 0.05).</p> <p>Conclusions</p> <p>These results suggested that excessive nitrosative stress occurred in EP and that Calv could be a marker of the disease activity.</p> |
| first_indexed | 2024-04-12T14:51:55Z |
| format | Article |
| id | doaj.art-9f18e9680ad14744a91e26105739682c |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-12T14:51:55Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
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| series | Respiratory Research |
| spelling | doaj.art-9f18e9680ad14744a91e26105739682c2022-12-22T03:28:24ZengBMCRespiratory Research1465-99212011-06-011218110.1186/1465-9921-12-81Increase of nitrosative stress in patients with eosinophilic pneumoniaAkamatsu KeiichiroMinakata YoshiakiYanagisawa SatoruHirano TsunahikoKoarai AkiraIchikawa TomohiroMatsunaga KazutoSugiura HisatoshiFurukawa KanakoKanda MasaeNishigai ManabuIchinose Masakazu<p>Abstract</p> <p>Background</p> <p>Exhaled nitric oxide (NO) production is increased in asthma and reflects the degree of airway inflammation. The alveolar NO concentration (Calv) in interstitial pneumonia is reported to be increased. However, it remains unknown whether NO production is increased and nitrosative stress occurs in eosinophilic pneumonia (EP). We hypothesized that nitrosative stress markers including Calv, inducible type of NO synthase (iNOS), and 3-nitrotyrosine (3-NT), are upregulated in EP.</p> <p>Methods</p> <p>Exhaled NO including fractional exhaled NO (FE<sub>NO</sub>) and Calv was measured in ten healthy subjects, 13 patients with idiopathic pulmonary fibrosis (IPF), and 13 patients with EP. iNOS expression and 3-NT formation were assessed by immunocytochemistory in BALf cells. The exhaled NO, lung function, and systemic inflammatory markers of the EP patients were investigated after corticosteroid treatment for 4 weeks.</p> <p>Results</p> <p>The Calv levels in the EP group (14.4 ± 2.0 ppb) were significantly higher than those in the healthy subjects (5.1 ± 0.6 ppb, p < 0.01) and the IPF groups (6.3 ± 0.6 ppb, p < 0.01) as well as the FE<sub>NO </sub>and the corrected Calv levels (all p < 0.01). More iNOS and 3-NT positive cells were observed in the EP group compared to the healthy subject and IPF patient. The Calv levels had significant positive correlations with both iNOS (r = 0.858, p < 0.05) and 3-NT positive cells (r = 0.924, p < 0.01). Corticosteroid treatment significantly reduced both the FE<sub>NO </sub>(p < 0.05) and the Calv levels (p < 0.01). The magnitude of reduction in the Calv levels had a significant positive correlation with the peripheral blood eosinophil counts (r = 0.802, p < 0.05).</p> <p>Conclusions</p> <p>These results suggested that excessive nitrosative stress occurred in EP and that Calv could be a marker of the disease activity.</p>http://respiratory-research.com/content/12/1/81Alveolar nitric oxidecorticosteroidfractional exhaled nitric oxideinducible type of nitric oxide synthase3-nitrotyrosine |
| spellingShingle | Akamatsu Keiichiro Minakata Yoshiaki Yanagisawa Satoru Hirano Tsunahiko Koarai Akira Ichikawa Tomohiro Matsunaga Kazuto Sugiura Hisatoshi Furukawa Kanako Kanda Masae Nishigai Manabu Ichinose Masakazu Increase of nitrosative stress in patients with eosinophilic pneumonia Respiratory Research Alveolar nitric oxide corticosteroid fractional exhaled nitric oxide inducible type of nitric oxide synthase 3-nitrotyrosine |
| title | Increase of nitrosative stress in patients with eosinophilic pneumonia |
| title_full | Increase of nitrosative stress in patients with eosinophilic pneumonia |
| title_fullStr | Increase of nitrosative stress in patients with eosinophilic pneumonia |
| title_full_unstemmed | Increase of nitrosative stress in patients with eosinophilic pneumonia |
| title_short | Increase of nitrosative stress in patients with eosinophilic pneumonia |
| title_sort | increase of nitrosative stress in patients with eosinophilic pneumonia |
| topic | Alveolar nitric oxide corticosteroid fractional exhaled nitric oxide inducible type of nitric oxide synthase 3-nitrotyrosine |
| url | http://respiratory-research.com/content/12/1/81 |
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